DETECTION OF URER AND UREC AMONG PROTEUS MIRABILIS

  • ABDUL KAREEM MUNEER ALATRASH Department of Biology, Faculty of Education for Girls, University of Kufa, Iraq
  • AHLAM KADHUM AL-YASSEEN Department of Biology, Faculty of Education for Girls, University of Kufa, Iraq

Abstract

Objective: This study aimed to investigate the correlation between ureR and ureC genes with the production of urease by Proteus mirabilis.

Methods: A total of 450 mid-stream urine samples have been collected from patients with urinary tract infection whom admitted to the hospitals in Annajaf Al-Ashraf province for consultancy during the period from October 2015 to February 2016.Out of 150 bacterial isolates, only 29 isolates were belong to P. mirabilis according to conventional methods (depending on microscopic and culturing examination as well as biochemical test) and molecular technique using 16SrRNA gene.

Results: The results of phenotypic and genotypic detection of urease in P. mirabilis showed that all isolates were able to produce urease and possess ureR and ureC that encodes to urease by appearing of amplicon with molecular weight 359 and 533 bp, respectively, when electrophoresed on 1% agarose gel.

Conclusion: A correlation has been found between ureR and ureC genes with the production of urease by P. mirabilis.

Keywords: Urease, Proteus mirabilis, ureR, ureC, Polymerase chain reaction technique.

References

1. Abbott SL. Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and other Enterobacteriaceae. In: Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA, editors. Manual of Clinical Microbiology. 9th ed. Washington, USA: ASM Press; 2007. p. 698-711.
2. Sekowska A, Janicka G, Wroblewska J, Kruszynska E. Prevalence of proteus mirabilis strains in clinical specimens and evaluation of their resistance to selected antibiotics. Pol Merkur Lekarski 2004;17(101):538-40.
3. Al-Duliami AA, Nauman NG, Hasan AS, Al-Azawi ZH. Virulence factors of Proteus mirabilis isolated from patients Otitis media in Baquba and its Peripheries. Diyala. J Med 2011;1(1):69-75.
4. Hasan AS, Al-Azawi ZH. Virulence factors of Proteus mirabilis isolated from patients otitis media in Baquba and its peripheries. Diyala J Med 2011;1(1):69-75.
5. Ali HH, Yousif MG. Detection of some virulence factors genes of Proteus mirabilis that isolated from urinary tract infection. Int J Adv Res 2015;3(1):156-63.
6. Armbruster CE, Mobley HL. Merging mythology and morphology: The multifaceted lifestyle of Proteus mirabilis. Nat Rev Microbiol 2012;10(11):743-54.
7. Baron EJ, Peterson LR, Finegold SM. Bailey and Scott’s Diagnostic Microbiology. 9th ed. U.S.A: The C.V. Mosby Company; 1994.
8. Broomfield RJ, Morgan SD, Khan A, Stickler DJ. Crystalline bacterial biofilm formation on urinary catheters by urease-producing urinary tract pathogens: A simple method of control. J Med Microbiol 2009;58:1367-75.
9. Coker C, Poor CA, Le X, Mobley HL. Pathogenesis of Proteus mirabilis urinary tract infection. Microb Infect 2000b;2:1497-509.
10. Dattelbaum DJ, Lockatell VC, Johnson ED, Mobley TL. UreR, the transcriptional activator of the Proteus mirabilis urease gene cluster, is required for urease activity and virulence in experimental urinary tract infection. J Infect Immun 2003;71:1026-30.
11. D’Orazio SE, Thomas V, Collins CM. Activation of transcription at divergent urea-dependent promoters by the urease gene regulator UreR. Mol Microbiol 1996;21(3):643-55.
12. El-Baghdady KZ, Abooulwafa M, Ghobashy MO, Gebreel HM. Plasmid mediated virulence factors of some Proteus isolates. Egypt Acad J Biol Sci 2009;1(1):7-22.
13. Gendlina I, Gutman DM, Thomas V, Collins CM. Urea-dependent signal transduction by the virulence regulator UreR. J Biol Chem 2002;277(40):37349-58.
14. Huang HS, Chen J, Teng LJ, Lai MK. Use of polymerase chain reaction to detect Proteus mirabilis and Ureaplasma urealyticum in urinary calculi. J Formos Med Assoc 1999;98(12):844-50.
15. Island MD, Mobley HL. Proteus mirabilis urease: Operon fusion and linker insertion analysis of ure gene organization, regulation, and function. J Bacteriol 1995;177(19):5653-60.
16. Jabri E, Carr MB, Hausinger RP, Karplus PA. The crystal structure of urease from Klebsiella aerogenes. Science 1995;268(5213):998-1004.
17. Jacobsen SM, Stickler DJ, Mobley HL, Shirtliff ME. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis. Clin Microbiol Rev 2008;21(1):26-59.
18. Jones BD, Mobley HL. Proteus mirabilis urease: Genetic organization, regulation, and expression of structural genes. J Bacteriol 1988;170(8):3342-9.
19. Jones SM, Yerly J, Hu Y, Ceri H, Martinuzzi R. Structure of Proteus mirabilis biofilms grown in artificial urine and standard laboratory media. J Microbiol Lett 2007;268(1):16-21.
20. Laurel SB, Janette MH, Xin L, Virginia CL, Stephanie DH, Richard JH, et al. Proteus mirabilis genes that contribute to pathogenesis of urinary tract infection: Identification of 25 signature tagged mutants attenuated at least 100 fold. J Infect Immun 2004;72:2922-38.
21. Li X, Lockatell CV, Johnson AC, Emody L, Coleman N, Hughes C. The role of swarm cell differentiation and multicellular migration in the uropathogenicity of Proteus mirabilis. J Infect Dis 2004;169:1155-8.
22. Li X, Lockatell CV, Johnson DE, Mobley HL. Identification of MrpI as the sole recombinase that regulates the phase variation of MR/P fimbria, a bladder colonization factor of uropathogenic Proteus mirabilis. Mol Microbiol 2002;45(3):865-74.
23. Limanskii A, Minukhin V, Limanskaia O. Specific-specific detection of Proteus vulgaris and Proteus mirabilis by the polymerase chain reaction. J Microbiol Epidemiol Immun 2005;3:9-33.
24. Lu JJ, Perng CL, Lee SY, Wan CC. Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid. J Clin Microbiol 2000;38(6):2076-80.
25. MacFaddin JF. Biochemical Tests for Identification of Medical Bacteria. 1st ed. Baltimore, USA: Williams and Wilkins; 2000.
26. Mobley LT, Chippendale GR. Hemagglutinin, urease and hemolysin production by Proteus mirabilis in clinical sources. J Infect Dis 1990;161:525-30.
27. Mobley HL, Island MD, Hausinger RP. Molecular biology of microbial ureases. Microbiol Rev 1995;59(3):451-80.
28. Nicholson EB, Concaugh EA, Foxall PA, Island MD, Mobley HL. Proteus mirabilis urease: Transcriptional regulation by UreR. J Bacteriol 1993;175(2):465-73.
29. Manoj K, Shikha C, Diljot KM, Neeru G, Sanjeevkumar C. Comparative antimicrobial efficacy evolution of new product elores against meropenem of gram-negative isolates. Asian J Pharm Clin Res 2015;8(4):251-4.
30. O’Hara CM, Brenner FW, Miller JM. Classification, identification, and clinical significance of Proteus, Providencia, and Morganella. Clin Microbiol Rev 2000;13(4):534-46.
31. Park IS, Hausinger RP. Diethylpyrocarbonate reactivity of Klebsiella aerogenes urease: Effect of pH and active site ligands on the rate of inactivation. J Protein Chem 1993;12(1):51-6.
32. Patel JB. 16S rRNA gene sequencing for bacterial pathogen identification in the clinical laboratory. Mol Diagn 2001;6(4):313-21.
33. Poore CA, Mobley HL. Differential regulation of the Proteus mirabilis urease gene 34. Ronald A. The etiology of urinary tract infection: Traditional and emerging pathogens. Am J Med 2002;113 Suppl 1A:14S-9.
35. Rozalski A, Torzewska A, Moryl M, Kwil I, Maszewska A, Ostrowska A, et al. Proteus sp. - An opportunistic bacterial pathogen-classification, swarming growth, clinical significance and virulence factors. Folia Biol Oecol 2013;8(1):1-17.
36. Sambrook J, Rusell DW. Molecular Cloning: A Laboratory Manual. 3rd ed. New York, N.Y: Cold Spring Harbor, Cold Spring Harbor Laboratory Press; 2001.
37. Samira N, Abouddihaj B, Nezha B, Kaotar N, Mohamed T, Abderraouf H, et al. Occurrence of carbapenemases and extended-spectrum beta-lactamses in uropathogenic enterobacterialceae isolated from a community setting, settat, morocco. Asian J Pharm Clin Res 2017;10(1):211-5.
38. Schabereiter-Gurtner C, Maca S, Rollenke S. 16S rRNA-based identification of bacteria from conjunctival swabs by PCR and DGGE fingerprinting. Invest Ophthalmol J Vis Sci 2001;42:1164.
39. Shoket H, Ali S, Gupta RK, Mishra VK. Occurrence of Proteus mirabilis associated with vegetable samples in Dehradun, Uttarakhand, India. Int J Curr Microbiol Appl Sci 2014;3(7):958-61.
Statistics
515 Views | 773 Downloads
Citatons
How to Cite
ABDUL KAREEM MUNEER ALATRASH, and AHLAM KADHUM AL-YASSEEN. “DETECTION OF URER AND UREC AMONG PROTEUS MIRABILIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 386-9, doi:10.22159/ajpcr.2017.v10i8.18987.
Section
Original Article(s)