PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF CALLISTEMON CITRINUS FOR ANTIDEPRESSANT ACTIVITY IN ALBINO MICE

  • Vijetha Pendyala Department of Pharmacognosy, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India
  • Santhrani Thaakur Departmet of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Viswavidyalayam, Tirupati, Andhra Pradesh, India.

Abstract

Objective: The present study was carried out to find out the antidepressant activity of different fractions of stems and leaves of Callistemon citrinus(CC). The efficacy of the fractions was compared with the standard reference drug imipramine.

Methods: All the studies were conducted according to the ethical guidelines of CPCSEA. Healthy adult albino mice weighing 20-30 g were used asexperimental animals. The leaves of CC were extracted with alcohol and fractionated with chloroform and petroleum ether. All the fractions weresubjected for preliminary phytochemical screening, using various qualitative tests. In the present investigation, tail suspension test (TST) and forcedswim test (FST) are selected as animal models for evaluation of antidepressant activity in albino mice.

Results: The preliminary phytochemical screening of CC has revealed the presence of carbohydrates, tannins, and flavonoids in a hydroalcoholicfraction. Chloroform fraction showed positive results toward flavonoids, alkaloids, tannins, glycosides, and steroids. Alcoholic and chloroform extract(100 and 200 mg/kg p.o.) of CC administered orally for 14 successive days had decreased the immobility periods significantly in a dose-dependentmanner in both TST and FST, showing significant antidepressant-like activity. The activities of the extracts were found to be comparable to imipraminein both FST and TST.

Conclusions: Although a number of synthetic drugs are being used as a standard treatment for clinically depressed patient, they have adverseeffects that can compromise the therapeutic treatment. In the traditional systems of medicine, many plants and formulations have been used to treatdepression for thousands of years. The results of the study indicate that CC can be used as an antidepressant herb.

Keywords: Callistemon citrinus, Antidepressant activity, Forced swim test, Tail suspension test, Depression.

Author Biographies

Vijetha Pendyala, Department of Pharmacognosy, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India
Pharmacognosy, associate professor
Santhrani Thaakur, Departmet of Pharmacology, Institute of Pharmaceutical Technology, Sri Padmavathi Mahila Viswavidyalayam, Tirupati, Andhra Pradesh, India.
Department of Pharmacology

References

1. Rang HP, Dale MM, Ritter JM. Pharmacology. 6th ed. Edinburgh: Churchill Livingstone; 2007. p. 553.
2. Dhingra D, Sharma A. A review on antidepressant plants. Nat Prod Radiance 2005;5(2):144-52.
3. Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi: Medical Publishers; 2008. p. 425.
4. Chopra RN, Nayar SI, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: CSIR; 1956. p. 128.
5. Sastri BN. The Wealth of India, Raw Materials and Industrial Products. New Delhi: CSIR-NISCAIR; 1956. p. 266.
6. Mishra LN, Hug F, Ahmad A, Dixit AK. Chemical composition of the essential oils of Callistemon lanceolatus DC and Callistemon polandii FM. Bailey J Essent Oil Res 1997;9(6):625.
7. Hashim FM, Shamy AM, Shehata AH. The flavonoids of the leaves of Callistemon lanceolatus DC. And Callistemon rigidus R. Br Bull Fac Pharm 1982;19(1):139.
8. Jirovetz L, Fleischacker W, Buchbauer G, Ngassoum MB. Analysis of the essential oils of Callistemon rigidus (Myrtaceae) from Cameroun by GC/FID and GC/MS. Sci Pharm 1997;65:315.
9. Ming JC, Verra RR, Fraisso DJ. Chemical composition of essential oil of Callistemon citrinus (Curtis) skeel. Reunion J Essent Oil Res 1998;10:429.
10. Sharma RK, Kotoky R, Bhattacharya PR. Volatile oil from the leaves of Callistemon lanceolatus grown in Northeastern India. Flavour Fragr J 2006;21(2):239.
11. Lounasmaa M, Puri HS, Widen CJ. A new dioleate compound from Callistemon lanceolatus. Phytochemistry 1977;16(11):1851.
12. Mohmoud FA, Mohrram MS, Marzouk MW, Salen MI. Polyphenolic constituents of Callistemon lanceolatus leaves. Pharmazie 2002;57(7):494.
13. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd ed. New York: Chapman and Hall; 1984. p. 1-10.
14. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 12th ed. Pune: Nirali Publications; 1999. p. 157-9.
15. Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: A new method for screening antidepressants in mice. Psychopharmacology (Berl) 1985;85(3):367-70.
16. Thierry B, Stéru L, Simon P, Porsolt RD. The tail suspension test: Ethical considerations. Psychopharmacology (Berl) 1986;90(2):284-5.
17. Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: A primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229(2):327-36.
18. Porsolt RD, Castagne V, Moser P. Behavioral assessment of antidepressant activity in rodents. In: Buccafusco JJ, editor. Methods of Behavior Analysis in Neuroscience. 2nd ed. Ch. 6. Boca Raton, (FL): CRC Press; 2009. Available from: http://www.ncbi.nlm.nih.gov/books/ NBK5222.
19. Santhosh P, Venugopal R, Nilakash S, Kunjbihari S, Mangala L. Anti depressant activity of methanolic extract of Passiflora foetida leaves in mice. Int J Pharm Pharm Sci 2011;3(1):112-5.
20. Umadevi P, Murugan S, Jennifer S, Subakanmani S. Evaluation of antidepressant like activity of Cucurbita pepo seed extracts in rats. Int J Curr Pharm Res 2010;3(1):108-13.
21. Sutar RC, Kasture SB, Kalaichelvan VK. Evaluation of antidepressant activity of leaf extracts of Holoptelea integrifolia (Roxb) planch in experimental animals. Int J Pharm Pharm Sci 2014;6(6):250-3.
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Pendyala, V., and S. Thaakur. “PHYTOCHEMICAL AND PHARMACOLOGICAL EVALUATION OF CALLISTEMON CITRINUS FOR ANTIDEPRESSANT ACTIVITY IN ALBINO MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 8, Aug. 2017, pp. 232-4, doi:10.22159/ajpcr.2017.v10i8.18998.
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