EVALUATION OF PHYTOCHEMICAL CONSTITUENTS AND ANTIMICROBIAL PROPERTIES OF MANGIFERA INDICA L. LEAVES AGAINST URINARY TRACT INFECTION-CAUSING PATHOGENS

Gyanranjan Mahalik; Sabuj Sahoo; Kunja Bihari Satapathy

doi:10.22159/ajpcr.2017.v10i9.19034

Authors

Gyanranjan Mahalik Post Graduate Department of Botany, Utkal University, Bhubaneswar - 751 004, Odisha, India.
Sabuj Sahoo Post Graduate Department of Biotechnology, Utkal University, Bhubaneswar - 751 004, Odisha, India
Kunja Bihari Satapathy Post Graduate Department of Botany, Utkal University, Bhubaneswar - 751 004, Odisha, India.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19034

Keywords:

Antimicrobial, Phytochemical, Soxhlet, Urinary tract infection

Abstract

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Â Objective: The main aim of this study was to evaluate the presence of different active compounds and their concentrations in different extracts of Mangifera indica L. leaves which can reduce the incidence of pathogens causing urinary tract infections (UTIs).

Methods: Leaves of M. indica L. were extracted in petroleum ether, methanol, and ethanol solvents by Soxhlet apparatus and screened for presence of secondary metabolites, antibacterial, and antifungal activities (agar well diffusion methods). The antimicrobial efficacy of different extracts was tested against UTI-causing organisms such as Escherichia coli, Shigella flexneri, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Candida albicans, Candida krusei, and Candida tropicalis.

Results: Leaves of M. indica revealed the presence of carbohydrates, proteins, amino acid, saponins, phenolics, tannins, sterols, and glycosides. Methanolic and ethanolic extracts of M. indica actively showed the presence of all the phytochemical constituents. All the test bacterial strains were found sensitive to ethanolic and aqueous extracts of M. indica leaves while P. mirabilis, K. pneumoniae, and S. flexineri showed resistance to petroleum ether extracts. Aspergillus niger was resistant to all the extracts of M. indica leaves, whereas C. tropicalis, C. krusei, and C. albicans demonstrated sensitivity toward ethanolic extracts. Higher concentrations of methanolic and petroleum ether extracts were capable of inhibiting the growth of fungal strains such as C. albicans, C. tropicalis, and C. krusei, whereas C. tropicalis was sensitive to all the extracts.

Conclusion: The extracts of M. indica leaves can be used as herbal cure to treat UTIs caused by different microorganisms.

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References

Amaechi I. Natural medicine potentials. Pharmaceutical society of Nigeria (PNS) Conference Proceedings, Abuja; 2003.

Ayodele QS. The effect of herbal remedies. Daily Champion 2003;

Kurian S, Josekumar VS. Phytochemical screening, antimicrobial activity and brine shrimp lethality bioassay of different extracts of Alysicarpus vaginalis Var. Nummularifolius (DC.) MIQ. (Family: Fabaceae). Int J Pharm Pharm Sci 2017;9(1):1.

Mahalik G, Satapathy KB, Sahoo S. Ethnobotanical survey of plants used in treatment of urinary disorders in Dhenkanal district of Odisha, India. J Environ Sci Toxicol Food Technol 2015;9(8):58-63.

Saxena HO, Brahmam M. Flora of Orissa. Vol. 1-4. Orissa: Orissa Forest Development Corporation; 1994-1996.

Tetyana P, Prozesky EA, Jager AK. Some medicinal properties of Cussonia and Schefflera species used in traditional medicine. S Afr J Bot 2002;68(1):51-4.

Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005;29(1):41-7.

Sinha S, Raghuwanshi R. Phytochemical screening and antioxidant potential of Eclipta prostrata (L) l-a valuable herb. Int J Pharm Pharm Sci 2016;8(3):255-60.

Dixit A, Rohilla A, Dixit J, Singh V. Qualitative analysis of various plant extracts of Alpinia officinarum. Int J Pharm Chem Biol Sci 2014;4(3):505-8.

Soares MO, Alves RC, Pires PC, Oliveira MB, Vinha AF. Angolan Cymbopogon citratus used for therapeutic benefits: Nutritional composition and influence of solvents in phytochemicals content and antioxidant activity of leaf extracts. Food Chem Toxicol 2013;60:413-8.

Mbata TI, Debiao LU, Saikia A. Antibacterial activity of the crude extract of Chinese green tea (Camellia sinensis) on Listeria monocytogenes. Afr J Biotechnol 2006;7:1571-3.

Norrell SA, Messley KE. Microbiology Laboratory Manual: Principles and Applications. New Jersey: Prentice-Hall; 1997.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.

Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000;31(4):886-91.

Doughari JH, El-Mahmood AM, Tyoyina I. Antimicrobial activity of leaf extracts of Senna obtusifolia L. Afr J Pharm Pharmacol 2008;2(1):7-13.