Subhashree Basu, Moumita Das, Anurupa Sen, Gouriprosad Datta



 Objective: The current study investigates the nephroprotective effect of Amorphophallus campanulatus against chronic alcohol-induced oxidative stress and tissue damage.

Methods: The rats were simultaneously supplemented with ethanolic extract of A. campanulatus along with ethanol (40% w/v)2 g/kg body weight/day for 30 days to evaluate the nephroprotective effect against alcohol toxicity. Renal antioxidant enzymes, serum urea, creatinine, and pro-inflammatory cytokines were assayed biochemically. Histomorphological and histochemical alterations were detected by Hematoxylin and Eosin, periodic acid Schiff, and Feulgen and Picrosirius stain, respectively. The degree of apoptotic cell death was examined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay technique.

Results: Serum urea, creatinine, pro-inflammatory cytokines, tissue TBARS, and activity of glutathione metabolizing enzymes were significantly (p<0.01) elevated, whereas cytosolic and mitochondrial superoxide dismutase, catalase, and levels of reduced glutathione were significantly (p<0.001) decreased in the EtOH group compared to control. However, ethanolic extract of A. campanulatus (ACE) supplementation to the EtOH rats reversed these effects to normal levels. Furthermore, degenerative changes in renal cells with alcohol treatment were minimized to nearness in architecture by ACE supplementation. Glycogen and deoxyribonucleic acid depletion, excess fibrosis due to collagen deposition, and increased apoptotic cell number were also restricted by ACE supplementation, with the higher dose being more promising.

Conclusion: Thus ethanol-induced nephrotoxicity was attenuated by ACE treatment by the antioxidative and antiapoptotic property of the extract. Such effects of the extract may be due to the probable presence of different bioactive components in the tuber. Hence, it can be used as a regular nutrient or therapeutic agent to protect the renal cells.


Apoptosis, Fibrosis, Nephrotoxicity, Oxidative stress, Pro-inflammatory cytokines, TUNEL.

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Apoptosis, Fibrosis, Nephrotoxicity, Oxidative stress, Pro-inflammatory cytokines, TUNEL.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 240-250

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Authors & Affiliations

Subhashree Basu
Department of Physiology, Rammohan College, 85A, Raja Rammohan Sarani, Kolkata, West Bengal, India

Moumita Das
Department of Physiology, Rammohan College, 85A, Raja Rammohan Sarani, Kolkata, West Bengal, India

Anurupa Sen
Department of Physiology, City College, 102/1, Raja Rammohan Sarani, Kolkata: 700009, West Bengal, India

Gouriprosad Datta
Department of Physiology, Rammohan College, 85A, Raja Rammohan Sarani, Kolkata, West Bengal, India

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