IN VITRO HEPATOPROTECTIVE EFFECT OF ECHINOCHLOA COLONA ON ETHANOL-INDUCED OXIDATIVE DAMAGE IN HEPG2 CELLS


Praneetha Pallerla, Durgaih G, Narsimha Reddy Y, Ravi Kumar B

Abstract


 

 Objective: The current study was aimed to evaluate the methanolic extract of caryopses of Echinochloa colona (ECME) for its in vitro hepatoprotective activity against ethanol in HepG2 cell lines.

Methods: In this regard, the cytotoxicity studies were conducted for the extract, ECME using 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay to determine the inhibitory concentration 50% value based on which, the doses 50, 100, and 200 μg/ml were selected for the hepatoprotective studies in HepG2 cell lines. The toxicity was induced using ethanol (100 mM). The in vitro hepatoprotective activity of the extract was assessed based on the changes in the level of biochemical parameters such as aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase.

Results: The extract, ECME has shown a dose-dependent cytoprotective activity with maximum protection at 200 μg/ml. The percentage cell viability of the extract, ECME at 200 μg/ml was more, i.e., 69.33% which was well comparable to that of standard drug, silymarin (100 μg/ml).

Conclusion: The study revealed that the extract had shown significant hepatoprotective activity at all the test doses against ethanol-induced cytotoxity assay.


Keywords


Ethanol, HepG2, Hepatoprotective activity, Echinochloa colona, 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide.

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About this article

Title

IN VITRO HEPATOPROTECTIVE EFFECT OF ECHINOCHLOA COLONA ON ETHANOL-INDUCED OXIDATIVE DAMAGE IN HEPG2 CELLS

Keywords

Ethanol, HepG2, Hepatoprotective activity, Echinochloa colona, 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide.

DOI

10.22159/ajpcr.2017.v10i9.19322

Date

01-09-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 9 September 2017 Page: 259-261

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Praneetha Pallerla
Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Telangana state, India
India

Durgaih G
Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Telangana state, India
India

Narsimha Reddy Y
Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Telangana state, India
India

Ravi Kumar B
Department of Pharmacognosy and Phytochemistry, University College of Pharmaceutical Sciences, Kakatiya University, Telangana state, India
India


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