EVALUATION OF A RAPID TEST TO DETECT CARBAPENEMASE PRODUCTION AMONG GRAM-NEGATIVE BACILLI.

  • Vishnu Nagalapuram Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
  • Jayalakshmi J Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.

Abstract

Objective: There is a need for an early detection of carbapenemase-producing Gram-negative bacilli to reduce the morbidity and mortality associated with it. The objective of this study is to evaluate the usefulness of the Carba NP test in detecting carbapenemase-producing Gram-negative bacilli.

Methods: This prospective experimental study was conducted in 2013 at PSG Institute of Medical Sciences and Research, Coimbatore. In this study, 120 consecutive Gram-negative bacilli clinical isolates identified as carbapenem resistant as per the Clinical and Laboratory Standard Institute guidelines were subjected to Carba NP.

Results: About 62.5% of the carbapenem-resistant isolates were found to be carbapenemase producers by modified Hodge test. The sensitivity of the Carba NP test was found to be 92% and the specificity was 100%. The most common bacterial isolate was Acinetobacter baumannii, and the most common sample was urine.

Conclusion: The Carba NP test is a simple inexpensive, rapid, reproducible, sensitive test that can be performed directly from the colonies. It improves early detection of patients infected or colonized with carbapenemase in the hospital.

Keywords: Carba NP test, Carbapenemase producer, Modified Hodge test.

Author Biography

Jayalakshmi J, Department of Microbiology, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
Professor, Department of Microbiology

References

1. Basavaraj CM, Jyothi P. Antimicrobial resistance of Pseudomonas aeruginosa strains from patients with urinary tract infections in SBMPMC hospital, Bijapur, India. Int J Pharm Pharm Sci 2014;6(9):479-81.
2. Nordmann P, Naas T, Poirel L. Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 2011;17:1791-8.
3. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; 25th Informational Supplement, M100-S25. Wayne, PA: Clinical and Laboratory Standards Institute; 2015.
4. Nordmann P, Poirel L, Dortet L. Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 2012;18:1503-7.
5. Hombach M, von Gunten B, Castelberg C, Bloemberg GV. Evaluation of the rapidec carba NP test for detection of carbapenemases in Enterobacteriaceae. J Clin Microbiol 2015;53:3828-33.
6. Dortet L, Poirel L, Nordmann P. Rapid identification of carbapenemase types in Enterobacteriaceae and Pseudomonas spp. by using a biochemical test. Antimicrob Agents Chemother 2012;56:6437-40.
7. Vasoo S, Cunningham SA, Kohner PC, Simner PJ, Mandrekar JN, Lolans K, et al. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 2013;51:3097-101.
8. Dortet L, Poirel L, Errera C, Nordmann P. CarbAcineto NP test for rapid detection of carbapenemase-producing Acinetobacter spp. J Clin Microbiol 2014;52:2359-64.
9. Pooja G, Sweta R, Sheela D, Dileep V. Colistin-carbapenem combination therapy against carbapenem resistant gram negative bacilli infections: Clinical and an in vitro synergy study. Int J Pharm Pharm Sci 2014;6:497-500.
Statistics
158 Views | 250 Downloads
How to Cite
Nagalapuram, V., and J. J. “EVALUATION OF A RAPID TEST TO DETECT CARBAPENEMASE PRODUCTION AMONG GRAM-NEGATIVE BACILLI.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 1, Jan. 2018, pp. 134-5, doi:10.22159/ajpcr.2018.v11i1.19605.
Section
Original Article(s)