SUPPRESSION OF MANGANESE SUPEROXIDE DISMUTASE ACTIVITY IN ROTENONE-TREATED HUMAN GLIOBLASTOMA T98G CELLS REDUCES CELL VIABILITY


Septelia Inawati Wanandi, Novi Silvia Hardiany, Nurjati Chairani Siregar, Mohamad Sadikin

Abstract


 

 Objective: Glioma is the most common human primary brain tumor which is highly resistant to oxidative stress-based anticancer. The aim of this study was to analyze the effect of rotenone-induced reactive oxygen species (ROS) on the modulation of manganese superoxide dismutase (MnSOD) expression and cell viability in human glioblastoma (GBM) T98G cells.

Methods: In this in vitro experimental study, T98G cells were treated with high-dose rotenone (0.5, 5, and 50 μM, respectively). Following rotenone treatment and intracellular ROS, both peroxide and superoxide radicals were determined. Moreover, we analyzed MnSOD mRNA expression, protein, and specific activity, as well as cell survival including viability, proliferation, apoptosis, and mitochondrial structure.

Results: High-dose rotenone treatment of T98G cells significantly increased intracellular ROS and MnSOD mRNA, but its protein and specific activity definitely decreased. The treatment also led to a reduction of cell viability, enhancement of apoptosis, and disruption of mitochondrial integrity.

Conclusion: Overproduction of ROS in rotenone-treated human GBM T98G cells could suppress the MnSOD protein level and activity even though mRNA synthesis has been increased. This modulation led to reduced survival of T98G cells through induction of cell death rather than inhibition of cell proliferation.


Keywords


Cell viability, Glioblastoma multiforme, Manganese superoxide dismutase, Rotenone.

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About this article

Title

SUPPRESSION OF MANGANESE SUPEROXIDE DISMUTASE ACTIVITY IN ROTENONE-TREATED HUMAN GLIOBLASTOMA T98G CELLS REDUCES CELL VIABILITY

Keywords

Cell viability, Glioblastoma multiforme, Manganese superoxide dismutase, Rotenone.

DOI

10.22159/ajpcr.2018.v11i1.19777

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 48-54

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Septelia Inawati Wanandi
Department of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia
Indonesia

Novi Silvia Hardiany
Department of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia
Indonesia

Nurjati Chairani Siregar
Department of Anatomic Pathology, Faculty of Medicine Universitas Indonesia

Mohamad Sadikin
Department of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia


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