HEPATOPROTECTIVE ROLE OF CISSUS QUADRANGULARIS ON LEAD ACETATE-INDUCED LIVER INJURY IN FEMALE WISTAR RATS


Neethu Jayan, Susmita Das, Santhosh Kumar R, Sabina Evan Prince, Asha Devi

Abstract


 

 Objectives: Several heavy metals like lead acetate can accumulate in the body due to exposure to the metal for a prolonged period. One of the possible mechanisms involved with lead toxicity is oxidative stress is for which liver is the target organ. The primary aim of this study was to examine the hepatoprotective role of methanolic stem extract from the herb Cissus quadrangularis on induced lead acetate liver injury in female Wistar rats.

Methods: The course of the study was for 14 days. The animals were separated into 5 groups: two being control and negative and the other 3 groups based on the dosage of the methanolic extract of the plant was given. The dosage of the plant extract given was once daily for all days of course study. During the last 7 days, lead acetate was injected in the animals (25 mg/kg of body weight). The sacrifice was done 14 days later and the blood and liver samples were taken, which is then used for different antioxidant enzymatic assays.

Results: Significant reduced (p < 0.05) antioxidant levels and increased lipid peroxidation levels were observed in lead acetate treated group which was ameliorated by the action of extract from Cissus quadrangularis fusiformis. Histopathological study also supported the finding.

Conclusion: The results of the different antioxidant enzymatic assays supported the hepatoprotective role of methanolic stem extract of the plant Cissus quadrangularis over induced lead acetate injury in wistar female rats.


Keywords


Antioxidant, Cissus quadrangularis, Hepatoprotective, Lead acetate.

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References


Goyer RA. Lead toxicity: Current concerns. Environ Health Perspect 1993;100:177-87.

Barbosa JF, Tanus-Santos JE, Gerlach RF, Parsons PJ. A critical review of biomarkers used for monitoring human exposure to lead: Advantages, limitations, and future needs. Environ Health Perspect 2005;113(12):1669-74.

Kirtikar KR, Basua BD. Indian Medicinal Plants. Vol. 2. Dehradun: International Book Distributors; 1987. p. 604.

Stohs SJ, Ray SD. A review and evaluation of the efficacy and safety of Cissus quadrangularis extracts. Phytother Res 2013;27(8):1107-14.

Mehta M, Kaur N, Bhutani KK. Determination of marker constituents from Cissus quadrangularis Linn. and their quantitation by HPTLC and HPLC. Phytochem Anal 2001;12(2):91-5.

Kumar G, Banu GS, Kannan V, Pandian MR. Antihepatotoxic effect of beta-carotene on paracetamol induced hepatic damage in rats. Indian J Exp Biol 2005;43(4):351-5.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S transferase activities in rat lung and liver. Biochim Biophys Acta 1979;582:67-78.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.

Marklund SL, Marklund G. Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.

Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.

Asha DS, Blossom B. Hepatoprotective role of Spirulina fusiformis on methotrexate induced liver injury in Wistar rats. Res J Biotechnol 2016;11(9):58-64.

Viswanatha Swamy AH, Kulkarni RV, Thippeswamy AH, Koti BC, Gore A. Evaluation of hepatoprotective activity of Cissus quadrangularis stem extract against isoniazid-induced liver damage in rats. Indian J Pharmacol 2010;42(6):397-400.

Babiak RM, Campello AP, Carnieri EG, Oliveira MB. Methotrexate: Pentose cycle and oxidative stress. Cell Biochem Funct 1998;16(4):283-93.

Unnati S. Cissus quadrangularis L.: Phytochemicals, traditional uses and pharmacological activities- a review. Int J Pharm Pharm Sci 2011;3(4):41-4.

Srinivasa RS, Sreedhara KR, Kumar MB. Preventive role of Emblica offcinalis and Cissus quadrangularis on bone loss in osteoporosis. Int J Pharm Pharm Sci 2013;5(4):465-70.




About this article

Title

HEPATOPROTECTIVE ROLE OF CISSUS QUADRANGULARIS ON LEAD ACETATE-INDUCED LIVER INJURY IN FEMALE WISTAR RATS

Topics

Biotechnology

Keywords

Antioxidant, Cissus quadrangularis, Hepatoprotective, Lead acetate.

DOI

10.22159/ajpcr.2017.v10i11.20018

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 182-185

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

12 Views | 50 Downloads

Authors & Affiliations

Neethu Jayan
Department of Biomedical Sciences, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu, India.
India

Susmita Das
Department of Biomedical Sciences, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu, India.
India

Santhosh Kumar R
Department of Biomedical Sciences, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu, India.
India

Sabina Evan Prince
Department of Biomedical Sciences, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu, India.
India

Asha Devi
Department of Biomedical Sciences, School of Bioscience and Technology, VIT University, Vellore, Tamil Nadu, India.
India


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