BIOPROSPECTING OF MARINE SPONGE (CALLYSPONGIA DIFFUSA) FOR ANTIBACTERIAL COMPOUND.


Bindu D, Vinoth Kumar T, Geetharamani D

Abstract


Objective: Marine sponges are a rich source of new antimicrobial drugs. The present study was aimed to evaluate the antibacterial activity of the marine sponge (Callyspongia diffusa) against human pathogenic bacteria and to analyze the presence of bioactive compounds in the sponge.

Methods: Antibacterial activity of the marine sponge C. diffuser was examined using petroleum ether, chloroform, n-butanol, methanol, ethanol, and water as solvents and tested against human pathogenic bacteria such as Esherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus by agar well diffusion method. Zoochemical analysis was performed to screen for the presence of secondary metabolites. Bioactive compounds were purified by thin layer chromatography (TLC) and were identified by gas chromatography-mass spectrometry (GC-MS) analysis.

Results: The results obtained show that the sponge extracts had significant antibacterial activity against the tested strains. The methanol extract was found to be the most effective and exhibited the highest potency against all pathogens tested. Zoochemical analysis revealed the presence of alkaloids, terpenoids, and sterols. In TLC, spots corresponding to a Rf value of 0.67 were found to possess antibacterial activity against the test bacteria. GC-MS chromatogram showed seven major peaks at retention time of 12.69, 13.81, 24.21, 24.65, 28.01, 28.93, 30.87 minutes. The mass of the compounds and fragments were matched with the National Institute of Standard and Technology (NIST) database for identification of probable compounds present in the sample. GC-MS analysis revealed the presence of bioactive compounds in the sponge.

Conclusion: This study confirms the marine natural species provides an excellent source of bioactive metabolites that can exploit to develop novel and potential therapeutic agents.


Keywords


Callyspongia diffusa, Solvent extraction, Antibacterial activity, Thin layer chromatography, Gas chromatography-mass spectrometry.

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About this article

Title

BIOPROSPECTING OF MARINE SPONGE (CALLYSPONGIA DIFFUSA) FOR ANTIBACTERIAL COMPOUND.

Topics

microbiology

Keywords

Callyspongia diffusa, Solvent extraction, Antibacterial activity, Thin layer chromatography, Gas chromatography-mass spectrometry.

DOI

10.22159/ajpcr.2018.v11i1.20078

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 150-153

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

78 Views | 50 Downloads

Authors & Affiliations

Bindu D
Department of Microbiology, Dr. N. G. P. Arts and Science College, Coimbatore, Tamil Nadu, India.
India

Vinoth Kumar T
Department of Microbiology, Dr. N. G. P. Arts and Science College, Coimbatore, Tamil Nadu, India.
India

Geetharamani D
Department of Microbiology, Dr. N. G. P. Arts and Science College, Coimbatore, Tamil Nadu, India.
India


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