Bindu D, Vinoth Kumar T, Geetharamani D


Objective: Marine sponges are a rich source of new antimicrobial drugs. The present study was aimed to evaluate the antibacterial activity of the marine sponge (Callyspongia diffusa) against human pathogenic bacteria and to analyze the presence of bioactive compounds in the sponge.

Methods: Antibacterial activity of the marine sponge C. diffuser was examined using petroleum ether, chloroform, n-butanol, methanol, ethanol, and water as solvents and tested against human pathogenic bacteria such as Esherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus by agar well diffusion method. Zoochemical analysis was performed to screen for the presence of secondary metabolites. Bioactive compounds were purified by thin layer chromatography (TLC) and were identified by gas chromatography-mass spectrometry (GC-MS) analysis.

Results: The results obtained show that the sponge extracts had significant antibacterial activity against the tested strains. The methanol extract was found to be the most effective and exhibited the highest potency against all pathogens tested. Zoochemical analysis revealed the presence of alkaloids, terpenoids, and sterols. In TLC, spots corresponding to a Rf value of 0.67 were found to possess antibacterial activity against the test bacteria. GC-MS chromatogram showed seven major peaks at retention time of 12.69, 13.81, 24.21, 24.65, 28.01, 28.93, 30.87 minutes. The mass of the compounds and fragments were matched with the National Institute of Standard and Technology (NIST) database for identification of probable compounds present in the sample. GC-MS analysis revealed the presence of bioactive compounds in the sponge.

Conclusion: This study confirms the marine natural species provides an excellent source of bioactive metabolites that can exploit to develop novel and potential therapeutic agents.


Callyspongia diffusa, Solvent extraction, Antibacterial activity, Thin layer chromatography, Gas chromatography-mass spectrometry.

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Radjasa OK, Sabdono A, Zocchi E. Richness of secondary metabolite producing marine bacteria associated with sponge Haliclona sp. Int J Pharm 2007;3:275-79.

Lee YK, Lee JH, Lee HK. Microbial symbiosis in marine sponges. J Microbiol 2001;39:254-64.

Fieseler L, Horn M, Wagner M, Hentschel U. Discovery of the novel candidate phylum “poribacteria” in marine sponges. Appl Environ Microbiol 2004;70:3724-32.

Azevedo LG, Peraza GG, Lerner C, Soares A, Murcia N, Muccillo-Baisch AL. Investigation of the anti-inflammatory and analgesic effects from an extract of Aplysina caissara, a marine sponge. Fundam Clin Pharmacol 2008;22:549-56.

Thakur NL, Muller W. Biotechnological potential of marine sponges. Curr Sci 2004;86:1506-12.

Hochmuth T, Niederkrüger H, Gernert C, Siegl A, Taudien S, Platzer M, et al. Linking chemical and microbial diversity in marine sponges: Possible role for poribacteria as producers of methyl-branched fatty acids. Chem Bio Chem 2010;11:2572-78.

Taylor MW, Radax R, Steger D, Wagner M. Sponge-associated microorganisms: Evolution, ecology, and biotechnological potential. Microbiol Mol Biol Rev 2007;71:295-347.

Wang G. Diversity and biotechnological potential of the sponge-associated microbial consortia. J Ind Microbiol Biotechnol 2006;33:545-51.

Gunasekera AS, Sfanos KS, Harmody DK, Pomponi SA, McCarthy PJ, Lopez JV. HBMMD: An enhanced database of the microorganisms associated with deeper water marine invertebrates. Appl Microbiol Biotechnol 2005;66:373-76.

Warad VB, Habbu P. Antimicrobial activity of Callyspongia diffusa (Marine sponge) associated endophytic bacterial strains. Int J Pharm Pharm Sci 2017;9:90-6.

Naz S, Jabeen S, Ilyas S, Manzoor F, Aslam F, Ali A. Antibacterial activity of Curcuma longa varieties against different strains of bacteria. Pak J Bot 2010;42:455-62.

Suresh M, Arularasan S, Kumaran NS. Screening on antimicrobial activity of Marine gastropods Babylonia zeylanica and Harpa conoidalis from mudasalodai, Southeast coast of India. Int J Pharm Pharm Sci 2012;4:552-6.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken HR. Manual of Clinical Microbiology. 6th ed. Washington, DC: ASM Press; 1995. p. 15-8.

Olurinola PF. A Laboratory Manual of Pharmaceutical Microbiology. Idu, Abuja, Nigeria: Gumming Publishing Company; 1996. p. 69-105.

Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005;29:41-7.

Harborne JB. Phytochemical Methods. London: Chapman and Hall Ltd.; 1973. p. 49-188.

Trease GE, Evans WC. Pharmacognosy. 13rd ed. London: Balliere Tindall; 1989. p. 176-80.

Available from:

Sugapriya M, Sudarsanam D. Free radical screening activity of marine sponge Aurora globostellata. Asian J Pharm Clin Res 2016;9:210-2.

Karwati A, Nomura J, Ramli N, Wahyudi AT. Cytotoxicity of crude extract from sponge associated bacteria against MOLT4 leukemia cell lines through apoptosis. Int J Pharm Pharm Sci 2015;7:246-9.

Pangal A, Gazge M, Mane V, Shaikh J. Various pharmacological aspects of coumarin derivatives: A review. Int J Pharm Biosci 2013;2:168-94.

Patil US, Deshmukh OS. GC-MS analysis of phytochemicals in the aqueous extract of Cyclea peltata. (Lam). Int J Sci Res 2015;4:350-51.

Thambidurai Y, Sudarshanam D, Habeek SK, Kizhakudan JK. Screening of bioactive compounds from marine sponges collected from Kovalam, Chennai. Asian J Pharm Clin Res 2017;10:231-36.

Babula P, Adam V, Havel L, Kizek R. Naphthoquinones and their pharmacological properties. Ceska Slov Farm 2007;56:114-20.

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Callyspongia diffusa, Solvent extraction, Antibacterial activity, Thin layer chromatography, Gas chromatography-mass spectrometry.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 150-153

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Authors & Affiliations

Bindu D
School of Biological Sciences, CMS College of Science and Commerce, Coimbatore, TamilNadu, India

Vinoth Kumar T
School of Biological Sciences, CMS College of Science and Commerce, Coimbatore, TamilNadu, India

Geetharamani D
PG and Research Department of Microbiology, Dr.N.G.P Arts and Science College, Coimbatore, TamilNadu, India.

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