ANTI-INFLAMMATORY ACTIVITY OF BACTERIA ASSOCIATED WITH MARINE SPONGE (HALICLONA AMBOINENSIS) VIA REDUCTING NO PRODUCTION AND INHIBITING CYCLOOXYGENASE-1, CYCLOOXYGENASE-2, AND SECRETORY PHOSPHOLIPASE A2 ACTIVITIES


Yosie Andriani, Leni Marlina, Habsah Mohamad, Hermansyah Amir, Siti Aisha M Radzi, Jasnizat Saidin

Abstract


 

 Objective: This study aimed to investigate the anti-inflammatory activity of methanol extract and fractions of bacteria associated with sponge (Haliclona amboinensis) and to evaluate their effect in reducing NO production and inhibiting cyclooxygenase-1 (COX-1), cyclooxgenase-2 (COX-2) and secretory phospholipase A2 (sPLA2) activity.

Methods: All bacterial isolates were cultured and supernatants were collected for the extraction of secondary metabolites using diaion HP-20 to obtain methanol extracts. Evaluation of cytotoxicity property was carried out on macrophage cell lines (RAW264.7) by 3-(4,5-dimethylthiazol- 2-yl) 2,5-diphenyl tetrazoliumbromide assay. Anti-inflammatory screening was done by inducible nitric oxide assay on RAW264.7 cell lines with lipopolysaccharide (LPS) stimulation. Dianion HP-20 was used to remove salt content. A selected methanol extract was subjected to further fractionations by C-18 reverse phase and their anti-inflammatory potential was evaluated by COX-1 and COX-2, and sPLA2 enzymatic assay.

Results: Seven methanol extracts showed no cytotoxic property against RAW 264.7 cell line (inhibitory concentration 50% > 30 μg/ml) and selected for anti-inflammatory screening assay. Result showed methanol extract HM 1.2 reduced NO production >80% and it has been selected for phytochemical screening, further fractionations and assay. Phytochemical screening showed alkaloids and terpenoids present in the HM 1.2. The HM 1.2 and its fractions (F1, F2, F1C1, F1C2, F1C3, and F1C4) were proven to inhibit COX-1, COX-2, and sPLA2 activity in the range of 60.516-116.886%, 20.554- 116.457%, and 70.2667-114.8148%, respectively.

Conclusions: This study revealed that bacteria associated with H. amboinensis have produced anti-inflammatory activity via reducing NO production and inhibiting COX-1, COX-2, and sPLA2 activity.

 


Keywords


Haliclona amboinensis, Associated bacteria, Anti-inflammatory, Inducible nitric oxide, Cyclooxygenase-1, Cyclooxygenase-2, Secretory phospholipase A.

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About this article

Title

ANTI-INFLAMMATORY ACTIVITY OF BACTERIA ASSOCIATED WITH MARINE SPONGE (HALICLONA AMBOINENSIS) VIA REDUCTING NO PRODUCTION AND INHIBITING CYCLOOXYGENASE-1, CYCLOOXYGENASE-2, AND SECRETORY PHOSPHOLIPASE A2 ACTIVITIES

Topics

Pharmaceutical; Biotechnology

Keywords

Haliclona amboinensis, Associated bacteria, Anti-inflammatory, Inducible nitric oxide, Cyclooxygenase-1, Cyclooxygenase-2, Secretory phospholipase A.

DOI

10.22159/ajpcr.2017.v10i11.20094

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 95-100

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

49 Views | 95 Downloads

Authors & Affiliations

Yosie Andriani
Institute of Marine Biotechnology, University of Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Malaysia

Leni Marlina
Institute of Marine Biotechnology, University of Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Malaysia

Habsah Mohamad
Institute of Marine Biotechnology, University of Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Malaysia

Hermansyah Amir
Educational Chemistry Program, Faculty of Teacher Training and Education, Bengkulu University, Bengkulu, Indonesia.
Indonesia

Siti Aisha M Radzi
Institute of Marine Biotechnology, University of Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia.
Malaysia

Jasnizat Saidin
School of Marine Sciences and Environmental, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia
Malaysia


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