HEPATOPROTECTIVE ACTIVITY OF VARIOUS EXTRACTS OF CLAUSENA DENTATA (WILLD.) ROEM. (SYN. CLAUSENA WILLDENOVII WIGHT. AND ARN.) RUTACEAE
Â Objectives: Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of Clausena dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study on pharmacological activities is in scarce. The present study was undertaken hepatoprotective activity of various extracts of C. dentata.
Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India. The dry powder of stem bark (2.5 kg) was first soaked in hexane for 24 hrs. The extract was suction filtered. This was repeated for two more days, and similar extracts were pooled together and concentrated at 40Â°C using Buchi R - 153 Rotavapor. The residual plant material was extracted successively with chloroform and methanol same manner. Preliminary phytochemical test and hepatoprotective activity of various extracts of C. dentata against carbon tetrachloride (CCl4) induced hepatotoxicity in rats were carried out. Biochemical and histopathological changes were observed.
Results: The highly significant (p<0.01) reduction in the levels of serums glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, alkaline phosphatase, and bilirubin observed in the study in rats simultaneously treated with C. dentata extracts and CCl4 as compared to CCl4 alone treated.
Conclusion: CCl4 induced hepatic damage was counteracted by the extracts of C. dentata. Changes were observed in enzymatic and histopathological level, when compared to CCl4 alone treated group.
2. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Allahabad, India: Lalit Mohan Basu Co.; 1933. p. 478.
3. Rao S, Ravindranath GS, Kumar VP. Volatile constituents of Clausena willdenovii-structures of the furan terpenes alpha-clausenan, diclausenan A and diclausenan B. Phytochemistry 1984;23:399-402.
4. Manohar VR, Pai MR, Sabitha P, Paipushpalatha, Mohandas R, Sheetal DU. Hepatoprotective activity of Phyllanthusniruri in thioacetamide induced hepatotoxicity in male Wistar rats. Int J Pharm Pharm Sci 2014;6(4):341-3.
5. Subramaniam A, Evans DA, Rajasekharan S, Pushpangadan P. Hepatoprotective activity of Trichopus zelanicus extract against paracetamol -induced hepatic damage in rats. Indian J Exp Biol 1998;36:385-9.
6. Barros AO, de Souza RS, Aranha ES, da Costa LM, de Souza TP, de Vasconcellos MC, et al. Antioxidant and hepatoprotective activities of Libidibia ferrea bark and fruit extracts. Int J Pharm Pharm Sci 2014;6(11):71-6.
7. Datta S, Basu K, Sinha S, Bhattacharyya P. Hepatoprotective effect of a protein isolated from Cajanus indicus Spreng. on carbon tetrachloride induced hepatotoxicity in mice. Indian J Exp Biol 1998;36:175-81.
8. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Publishers; 1988. p. 278.
9. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Publishers; 1998. p. 293.
10. Singh K, Khanna AK, Chander R. Hepatoprotective activity of ellagic acid against carbon tetrachloride induced hepatotoxicity in rats. Indian J Exp Biol 1999;37:1025-6.
11. Tietz NW. Fundamentals of Clinical Chemistry. Philadelphia, PA, U.S.A: W.B. Saunders and Company; 1976. p. 602.
12. Reitaman S, Frankel S. Quantitative determination of serum glutamate pyruvate transaminase in serum. Am J Clin Pathol 1957;28:56-63.
13. Jendrassike L, Grof P. Vereinfachte photometrische methoden zur bestimmung des blutbilirubins. Biochem Z 1938;297:81.
14. Snedecor GW, Cochran WG. Statistical Methods. New Delhi, India: IBM Publishing Company; 1979. p. 360.
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