ANTIMICROBIAL, INSECTICIDAL, AND ANTIRADICAL ACTIVITY OF SOLANUM VIRGINIANUM L. (SOLANACEAE)
Â Objectives: The present study was conducted to determine antimicrobial, antiradical, and insecticidal activity of methanolic extract of the whole plant of Solanum virginianum L. (Solanaceae).
Methods: Extraction of shade dried and powdered plant material was carried out by maceration process. Antibacterial and antifungal activities of extract were determined by agar well diffusion and poisoned food technique, respectively. Antiradical activity of extract was assessed by 1,1-diphenyl- 2-picrylhydrazyl (DPPH) and 2,2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS) free radical scavenging assays. Insecticidal activity, in terms of larvicidal activity, was evaluated against 2nd and 3rd instar larvae of Aedes aegypti.
Results: The extract exhibited inhibitory activity against all bacteria. Gram-positive bacteria showed greater susceptibility to extract when compared to Gram-negative bacteria. The extract was effective in inhibiting the mycelial growth of all test fungi with marked activity against Curvularia sp. and Alternaria sp. The extract scavenged DPPH and ABTS radicals dose dependently with an inhibitory concentration value of 45.66 and 7.37Î¼g/ml, respectively. The extract showed concentration-dependent larvicidal activity with high lethal activity observed against 2nd instar larvae with lethal concentration value of 0.82 mg/ml.
Conclusion: In suitable form, the plant can be used as an effective agent against microbial infections and oxidative damage and to control insect vectors which transmit several diseases.
4. Nagori K, Sharma M, Agrawal A, Agarwal AK, Sharma A, Verma H, et al. General awareness on Allopathic, Ayurvedic and Homeopathic system of medicine in Chhattisgarh, India. Int J Pharm Pharm Sci 2011;3(4):159-62.
5. Sahu PK, Masih V, Gupta S, Sen DL, Tiwari A. Ethnomedicinal plants used in the healthcare systems of tribes of Dantewada, Chhattisgarh India. Am J Plant Sci 2014;5:1632-43.
6. Swamy SH, Asha MM, Chaithra M, Vivek MN, Kambar Y, Kekuda PT. Antibacterial activity of flower extract of Caesalpinia pulcherrima, Delonix regia and Peltaphorum ferrugineum against urinary tract pathogens. Int Res J Biol Sci 2014;3(4):80-3.
7. Bhat GK. Flora of South Kanara. Mangalore: Akriti Prints; 2014. p. 723.
8. Rita P, Animesh DK. An updated overview on Solanum xanthocarpum Schrad and Wendl. Int J Res Ayurveda Pharm 2011;2(3):730-5.
9. Rane MH, Sahu NK, Ajgoankar SS, Teli NC, Verma DR. A holistic approach on review of Solanum virginianum. L. Res Rev J Pharm Pharm Sci 2014;3(3):1-4.
10. Reddy NM, Reddy RN. Solanum xanthocarpum chemical constituents and medicinal properties: A review. Sch Acad J Pharm 2014;3(2):146-9.
11. Manjunatha BK, Krishna V, Pullaiah T. Flora of Davanagere District, Karnataka, India. New Delhi: Regency Publications; 2004. p. 281-2.
12. Kayani S, Ahmad M, Zafar M, Sultana S, Khan MP, Ashraf MA, et al. Ethnobotanical uses of medicinal plants for respiratory disorders among the inhabitants of Gallies - Abbottabad, Northern Pakistan. J Ethnopharmacol 2014;156:47-60.
13. Kumar PR. Ethno medicinal plants used for oral health care in India. Int J Herb Med 2014;2(1):81-7.
14. Rana S, Prakash V, Sagar A. Antibacterial activity of Solanum xanthocarpum leaf extract. Int J Curr Microbiol Appl Sci 2016;5(4):323-8.
15. Pardhi P, Jain AP, Ganeshpurkar A, Rai G. Anti-microbial, anti-oxidant and anthelmintic activity of crude extract of Solanum xanthocarpum. Pharmacogn J 2010;2(11):400-4.
16. Dinanath PD, Gurumeet WC. Antibacterial, antioxidant and anti-inflammatory studies of leaves and roots of Solanum xanthocarpum. Unique J Ayurvedic Herb Med 2013;1(3):59-63.
17. Shah MA, Khan H, Muhammad N, Khan F, Shahnaz, Muhammad A, et al. Cytotoxic, antioxidant and phyototoxic effect of Solanum surattense Burm f fruit extracts. Int J Pharmacogn Phytochem 2013;28(2):1154-8.
18. Kumar SR, Hariprasanth RJ, Siddharth PM, Gobinath M, Rajukutty C. Evaluation of the antioxidant, antimicrobial, antidiabetic and hemolytic activity of organically grown Solanum nigrum and Solanum xanthocarpum. Int J Curr Pharm Rev Res 2016;7(5):296-9.
19. Rajakumari M, Selvi KK. Effect of fruit of Solanum xanthocarpum as immuno stimulant on fish Channa striatus. Int J Sci Technol 2016;4(12):76-87.
20. Kekuda TR, Raghavendra HL, Solomon T, Duressa D. Antifungal and antiradical potential of Moringa stenopetala (Baker f.) Cufod (moringaceae). J Biosci Agric Res 2016;11(1):923-9.
21. Murthy JM, Rani PU. Biological activity of certain botanical extracts as larvicides against the yellow fever mosquito, Aedes aegypti. L. J Biopestic 2009;2(1):72-6.
22. Telban B. The role of medical ethno botany in ethno medicine: A New Guinea example. J Ethnobiol 1988;8(2):149-69.
23. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12(4):564-82.
24. Valgas C, de Souza SM, SmÃ¢nia EF, SmÃ¢nia A Jr. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol 2007;38(2):369-80.
25. Hassan BA. Medicinal plants (importance and uses). Pharm Anal Acta 2012;3:10.
26. Saini V, Middha A, Kinger HK, Rathore MS, Rathore SG. Antibacterial and antifungal activities of Solanum xanthocarpum leaf. Int J Plant Sci 2006;1(2):367-8.
27. Sheeba E. Antibacterial activity of Solanum surattense Burm. f. Kathmandu Univ J Sci Eng Technol 2010;6(1):1-4.
28. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71-9.
29. Albera A, Lemessa F, Muleta D. The antifungal activity of some medicinal plants against coffee berry disease caused by Colletotrichum kahawae. Int J Agric Res 2011;6(3):268-79.
30. Prince L, Prabakaran P. Antifungal activity of medicinal plants against plant pathogenic fungus Colletotrichum falcatum. Asian J Plant Sci Res 2011;1(1):84-7.
31. Neela FA, Sonia IA, Shamsi S. Antifungal activity of selected medicinal plant extract on Fusarium oxysporum Schlecht the causal agent of fusarium wilt disease in tomato. Am J Plant Sci 2014;5:2665-71.
32. Minz S, Samuel CO, Tripathi SC. The effect of plant extracts on the growth of wilt causing fungi Fusarium oxysporum. IOSR J Pharm Biol Sci 2012;4(1):13-6.
33. Rodino S, Butu M, Petrache P, Butu A, Cornea CP. Antifungal activity of four plants against Alternaria alternata. Sci Bull F Biotechnol 2014;18:60-5.
34. Shubha KS, Sumana K, Lakshmidevi L. Antifungal activity of Solanum xantocarpum Sch and Wend and Picrorhiza kurroa Royle ex Benth against some clinical dermatophytes. Int J Curr Microbiol Appl Sci 2016;5(2):236-44.
35. Abbas K, Niaz U, Hussain T, Saeed MA, Javaid Z, Idrees A, et al. Antimicrobial activity of fruits of Solanum nigrum and Solanum xanthocarpum. Acta Pol Pharm 2014;71(3):415-21.
36. Perumal B, Murugesan S. Study of antimicrobial activity of Solanum xanthocarpum sp. Int J Curr Trend Res 2015;4(1):23-8.
37. Mamta, Pimpalgaonkar R, Dalei J. Qualitative evaluation of phytochemicals and antifungal activity of Solanum xanthocarpumâ€ (Schrad. &Wendl.). IOSR J Pharm Biol Sci 2016;11(3):99-104.
38. Shiv G, Gaherwal S, Shrivastava CS, Wast N. Antifungal activity of Aegle marmelos, Calotropis procera and Solanum xanthocarpum extract against Aspergillus niger, Candida albicans and Phenerochaete chrysosporium. Eur J Exp Biol 2015;5(2):81-9.
39. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
40. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48(4):412-22.
41. Muruhan S, Selvaraj S, Viswanathan PK. In vitro antioxidant activities of Solanum surattense leaf extract. Asian Pac J Trop Biomed 2013;3(1):28-34.
42. Archana C, Jacob J. Phytochemical investigation and anti-oxidant studies on the roots of Solanum xanthocarpum Linn. Asian J Phytomed Clin Res 2015;3(1):32-6.
43. Harve G, Kamath V. Larvicidal activity of plant extracts used alone and in combination with known synthetic larvicidal agents against Aedes aegypti. Indian J Exp Biol 2004;42(12):1216-9.
44. Ramar R, Ignacimuthu S, Manonmani P, Murugan K. Adulticidal activity of botanical oils by impregnated paper assay against Culex quinquefasciatus say. Int J Pharm Pharm Sci 2017;9(5):156-60.
45. Yousaf A, Zuharah WF. Lethal response of the dengue vectors to the plant extracts from family Anacardiaceae. Asian Pac J Trop Biomed 2015;5(10):812-8.
46. Ruiz-Guerrero R, RodrÃguez-PÃ©rez MA, Norzagaray-Campos M. Toxicity of Mexican native plant extracts against larvae of Aedes aegypti (Diptera: Culicidae). Asian Pac J Trop Biomed 2015;5(4):287-91.
47. Bansal SK, Singh KV, Kumar S. Larvicidal activity of the extracts from different parts of the plant Solanum xanthocarpum against important mosquito vectors in the arid region. J Environ Biol 2009;30(2):221-6.
48. Mohan L, Sharma P, Srivastava CN. Combination larvicidal action of Solanum xanthocarpum extract and certain synthetic insecticides against filarial vector, Culex quinquefasciatus (SAY). Southeast Asian J Trop Med Public Health 2010;41(2):311-9.
49. Mahesh Kumar P, Murugan K, Kovendan K, Subramaniam J, Amaresan D. Mosquito larvicidal and pupicidal efficacy of Solanum xanthocarpum (Family: Solanaceae) leaf extract and bacterial insecticide, Bacillus thuringiensis, against Culex quinquefasciatus say (Diptera: Culicidae). Parasitol Res 2012;110(6):2541-50.
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