HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST ETHANOL INDUCED HEPATOTOXICITY IN RATS
Keywords:Terminalia chebula, Ethanol, Hepatotoxicity, Silymarin, Extract of Terminalia chebula
Â Objective: The purpose of this study was to evaluate the effect of Terminalia chebula fruit extract on liver antioxidant enzymes in ethanol-induced hepatotoxicity in rats.
Method: Rats were divided into six different groups each having six. Group 1 served as a control, Group 2 received 40% ethanol (2 ml/100 g, oral), in sterile water, Groups 4, 5, and 6 served as extract treatment groups and received 50, 100, and 200 mg/kg, orally, ethanolic fruit extract of T. chebula (TCE) and Group 3 served as standard group and received silymarin 25 mg/kg orally. All the treatment protocols followed 21 days, and after which rats were sacrificed, the liver was taken for antioxidant and histological studies, respectively.
Results: The ethanol-treated group rats (G2) showed variable decrease in antioxidant parameter (catalase, glutathione, and glutathione reductase) levels. Administration of ethanolic TCE significantly prevented ethanol-induced elevation in the levels of malondialdehyde lipid peroxidation and decreased antioxidant parameters in experimental groups of rats. The effect of extract was compared with a standard drug, silymarin. The changes in antioxidant parameters were supported by histological profile.
Conclusion: It is concluded that the ethanolic fruit TCE protects against ethanol-induced oxidative liver injury in rats.
Mandayam S, Jamal MM, Morgan TR. Epidemiology of alcoholic liver disease. Semin Liver Dis 2004;24(3):217-32.
Roizen R, Kerr WC, Fillmore KM. Cirrhosis mortality and per capita consumption of distilled spirits, United States, 1949-1994: trend analysis. West J Med 1999;171(2):83-7.
Suguna L, Singh S, Sivakumar P, Sampath P, Chandrakasan G. Influence of Terminalia chebula on dermal wound healing in rats. Phytother Res 2002;16(3):227-31.
Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol Res Health 2003;27(4):277-84.
Thurman RG. Mechanism of hepatic toxicity: II. Alcoholic liver injury involves activation of Kupffer cells by endotoxin. Am J Physiol 1998;275:G605-11.
Hazra B, Sarkar R, Biswas S, Mandal N. Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia bellirica and Emblica officinalis. BMC Complement Altern Med 2010;10:20.
Kokate CK, Purohith AP, Gokhale SB. Pharmacognosy. Pune: Nirali Prakashan; 1990.
Ghosh MN. Fundamentals of Experimental Pharmacology. Kolkata: Scientific Book Agency; 1984.
Bergmeyer HU, HÃ¸rder M, Rej R. International Federation of Clinical Chemistry (IFCC) Scientific Committee, Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase (L-alanine: 2-oxoglutarate aminotransferase, EC 220.127.116.11). J Clin Chem Clin Biochem 1986;24(7):481-95.
Georg LE. Tissue sulfhydryl group. Arch Biochem Biophy 1959;82(1):70-7.
Saravanan N, Nalini N. Antioxidant effect of Hemidesmus indicus on ethanol-induced hepatotoxicity in rats. J Med Food 2007;10(4):675-82.
Jain A, Soni M, Deb L, Jain A, Rout SP, Gupta VB, et al. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. Leaves. J Ethnopharmacol 2008;115(1):61-6.
Evan PS, Mahaboobkhan R, Mahima V, Dhanalakshmi N, Meenakshi R, Poornima P, et al. Hepatoprotective and antioxidant potential of Withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci 2013;10(2):648-51.
Monzoni A, Masutti F, Saccoccio G, Bellentani S, Tiribelli C, Giacca M. Genetic determinants of ethanol-induced liver damage. Mol Med 2001;7(4):255-62.
Rajalingam D, Varadharajan R, Palani S. Evaluation of hepatoprotective and antioxidant effect of Combretum albidum g. Don against ccl4 induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2016;8(9):218-23.
Valko M, Leibfritz D, Moncol J. Free radical and antioxidants in normal physiological functions and human disease. J Biocell 2006;7(1):45-78.
Chance B, Greenstein DS, Roughton FJ. The mechanism of catalase action. I. Steady-state analysis. Arch Biochem Biophys 1952;37(2):301-21.
Venditti P, Balestrieri M, Di Meo S, De Leo T. Effect of thyroid state on lipid peroxidation, antioxidant defences, and susceptibility to oxidative stress in rat tissues. J Endocrinol 1997;155(1):151-7.
Stewart S, Jones D, Day CP. Alcoholic liver disease: New insights into mechanisms and preventative strategies. Trends Mol Med 2001;7(9):408-13.
Reinke LA, Lai EK, DuBose CM, McCay PB. Reactive free radical generation in vivo in heart and liver of ethanol-fed rats: Correlation with radical formation in vitro. Proc Natl Acad Sci U S A 1987;84(24):9223-7.
Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature 2000;408(6809):239-47.
Priyanka S, Anupam DT, Manabendra DC. Antioxidant potential profile of Pajanelia longifolia (Willd.) k. Schuman; Potential new sources of natural antioxidant. Asian J Pharm Clin Res 2017;10(6):184-8.
Sarita MK, Abhijit B. Standardization of model of induction of hepatotoxicity with anti tuberculosis drugs in wistar albino rats. Asian J Pharm Clin Res 2017;10(6):150-3.
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