POTENTIAL ANTI-INFLAMMATORY ACTIVITY OF PLUMBAGO ZEYLANICA

  • Vetriselvan Subramaniyan Department of Pharmacology, Faculty of Medicine, MAHSA University, Kuala Lumpur, Malaysia.
  • Velmurugan Paramasivam Department of Biotechnology, SRM University, Chennai, Tamil Nadu, India

Abstract

 

 Objective: To determine the anti-inflammatory activity of dichloromethane extract of Plumbago zeylanica (DMEPZ), and its possible mechanism of action.

Methods: Male Wistar rats (180-200 g) under controlled standard conditions (24±1°C, 55-58 humidity and 12 hrs light/dark cycle). The groups were divided into 5 groups (n=6/group) and assigned as positive control, negative control, and standard and two different test dose groups of P. zeylanica. Paw edema induced by subplantar injection of 0.1 mL of carrageenan (suspended in 1% carboxymethyl cellulose) into the right hind paw in all groups except negative control group. Granuloma induced by cotton pellets (10±1 mg) were implanted into groin region of each rat. The groups were divided into 4 groups (n=6/group) and assigned as possitive control, two different test dose groups of P. zeylanica and standard.

Results: Oral administration of DMEPZ shown a significant (p<0.05) dose-dependent protection against carrageenan-induced paw edema. At 1st hr, P. zeylanica shown an inhibition effect of edema in the different doses of 250 mg/kg and 500 mg/kg were found to be 28.57 and 31.79%, respectively. At 3rd hrs, the paw edema inhibition was found to be 30.70 and 40.15%, respectively. Diclofenac (25 mg/kg) had effect of 34.10 and 41.73% (p<0.001) inhibition of paw edema at in 1 and 3 hrs. P. zeylanica 500 mg/kg showed percentage inhibition of wet and dry cotton pellet granuloma in rats 55.84% and 47.92%, respectively.

Conclusion: Thus, the present study revealed that the DMEPZ offered significant protection against inflammation.

Keywords: Plumbago zeylanica, Inflammation, Anti-inflammatory, Anti-granuloma activity.

References

1. Tabassum N, Hamdani M. Plants used to treat skin diseases. Pharmacogn Rev 2014;8(15):52-60.
2. Shankar R, Lavekar GS, Deb S, Sharma BK. Traditional healing practice and folk medicines used by Mishing community of North East India. J Ayurveda Integr Med 2012;3(3):124-9.
3. Maurya SK, Seth A, Laloo D, Singh NK, Gautam DN, Singh AK. Sodhana: An Ayurvedic process for detoxification and modification of therapeutic activities of poisonous medicinal plants. Anc Sci Life 2015;34(4):188-97.
4. Kumar D, Ganguly K, Hegde HV, Patil PA, Roy S, Kholkute SD. Activity of Plumbago zeylanica Linn. Root and Holoptelea integrifolia Roxb. Bark pastes in acute and chronic paw inflammation in Wistar rat. J Ayurveda Integr Med 2014;5:33-7.
5. Kishore N, Mishra BB, Tiwari KV, Tripathi V. An account of phytochemicals from Plumbago zeylanica (Family: Plumbaginaceae): A natural gift to human being. Chron Young Sci 2012;3:178-98.
6. Parekar RR, Bolegave SS, Marathe PA, Rege NN. Experimental evaluation of analgesic, anti-inflammatory and anti-platelet potential of Dashamoola. J Ayurveda Integr Med 2015;6(1):11-8.
7. Ariyanathan S, Saraswathy A, Rajamanickam GV. Quality control standards for the roots of three plumbago species. Indian J Pharm Sci 2010;72(1):86-91.
8. Shukla P, Singh RK. Toxicogenomics of phenylhydrazine induced hematotoxicity and its attenuation by plumbagin from Plumbago zeylanica. Pharmacogn Mag 2015;11 Suppl 3:S380-7.
9. Ittiyavirah SP, Ruby R. Effect of hydro-alcoholic root extract of Plumbago zeylanica l alone and its combination with aqueous leaf extract of Camellia sinensis on haloperidol induced Parkinsonism in Wistar rats. Ann Neurosci 2014;21(2):47-50.
10. Dai Y, Hou LF, Chan YP, Cheng L, But PP. Inhibition of immediate allergic reactions by ethanol extract from Plumbago zeylanica stems. Biol Pharm Bull 2004;27(3):429-32.
11. Bopaiah CP, Pradhan N. Central nervous system stimulatory action from the root extract of Plumbago zeylanica in rats. Phytother Res 2001;15(2):153-6.
12. Jain P, Sharma HP, Basri F, Baraik B, Kumari S, Pathak C. Pharmacological profiles of ethno-medicinal plant: Plumbago zeylanica l. A review. Int J Pharm Sci Rev Res 2014;24:157-63.
13. Kumar D, Patil PA, Roy S, Kholkute SD, Hegde HV, Nair V. Comparative toxicity profiles of Plumbago zeylanica L. Root petroleum ether, acetone and hydroalcoholic extracts in Wistar rats. Ayu 2015;36:329-34.
14. Zholos AV. TRP channels in respiratory pathophysiology: The role of oxidative, chemical irritant and temperature stimuli. Curr Neuropharmacol 2015;13(2):279-91.
15. Ma Y, Li Y, Li X, Wu Y. Anti-inflammatory effects of 4-methylcyclopentadecanone on edema models in mice. Int J Mol Sci 2013;14(12):23980-92.
16. Ricciotti E, Fitzgerald AG. Prostaglandins and Inflammation. Arterioscler Thromb Vasc Biol 2011;31:986-1000.
17. Wilgus TA, Roy S, McDaniel JC. Neutrophils and wound repair: Positive actions and negative reactions. Adv Wound Care (New Rochelle) 2013;2(7):379-88.
18. Ameni D, Baghiani A, Boumerfeg S, Dahamna S, Khennouf S, Abu Zarga MH, et al. Phytochemical profiles, antioxidant capacity and protective effect against AAPH-induced mouse erythrocyte damage by Daphne Gnidium L. Shoots extracts. Int J Pharm Pharm Sci 2015;11:148-56.
19. Raj J, Chandra M, Dogra TD, Pahuja M, Raina A. Determination of median lethal dose of combination of endosulfan and cypermethrin in wistar rat. Toxicol Int 2013;20(1):1-5.
20. Tilak JC, Adhikari S, Devasagayam TP. Antioxidant properties of Plumbago zeylanica, an Indian medicinal plant and its active ingredient, plumbagin. Redox Rep 2004;9(4):219-27.
21. Noh MK, Jung M, Kim SH, Lee SR, Park KH, Kim DH, et al. Assessment of IL-6, IL-8 and TNF-a levels in the gingival tissue of patients with periodontitis. Exp Ther Med 2013;6(1):847-51.
22. Chattopadhyay P, Hazarika S, Dhiman S, Upadhyay A, Pandey A, Karmakar S, et al. Vitex negundo inhibits cyclooxygenase-2 inflammatory cytokine-mediated inflammation on carrageenan-induced rat hind paw edema. Pharmacognosy Res 2012;4(3):134-7.
23. Haj Said AA, El Otmani IS, Derfoufi S, Moussa AB. Highlights on nutritional and therapeutic value of stinging nettle (Urtica dioica). Int J Pharm Pharm Sci 2015;7:8-14.
24. Viljoen A, Mncwangi N, Vermaak I. Anti-inflammatory iridoids of botanical origin. Curr Med Chem 2012;19(14):2104-27.
25. Nesa L, Munira S, Mollika S, Islam M, Choin H, Chouduri AU, et al. Evaluation of analgesic, anti-inflammatory and CNS depressant activities of methanolic extract of Lawsonia inermis barks in mice. Avicenna J Phytomed 2014;4(4):287-96.
26. Khatun H, Majumder R, Al Mamun, Alam EK, Jami SI, Alam B. Preliminary pharmacological activity of the methanolic extract of Premna integrifolia barks in rats. Avicenna J Phytomed 2014;4(3):215-24.
27. Schomberg D, Ahmed M, Miranpuri G, Olson J, Resnick DK. Neuropathic pain: Role of inflammation, immune response, and ion channel activity in central injury mechanisms. Ann Neurosci 2012;19(3):125-32.
28. Gupta M, Mazumder UK, Gomathi P, Selvan VT. Antiinflammatory evaluation of leaves of Plumeria acuminata. BMC Complement Altern Med 2006;6:36.
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Subramaniyan, V., and V. Paramasivam. “POTENTIAL ANTI-INFLAMMATORY ACTIVITY OF PLUMBAGO ZEYLANICA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 10, Sept. 2017, pp. 372-5, doi:10.22159/ajpcr.2017.v10i10.20357.
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