EVALUATION OF IN VITRO CYTOTOXIC EFFECT OF VIOLACEIN PRODUCED BY NOVEL ISOLATE CHROMOBACTERIUM VACCINII CV5 AGAINST THE CERVICAL AND LUNG CANCER CELL
Â Objectives: This study investigates the in vitro anticancer activity of the violacein extracted from the Chromobacterium vaccinii CV5.
Methods: Natural colorants or dyes derived from flora to fauna are believed to be safe because of nontoxic, noncarcinogenic, and biodegradable in nature. There are a number of natural pigments, but only a few are available in sufficient quantities for industrial production. The cytotoxicity activity of pigment was assessed against the cervical (HeLa) and lung cancer (A549) cell lines using the MTT assay and there by potential cytotoxic activity exhibited by the pigment was identified.
Results: The result of the pigment shows potent anticancer activity on the two cancer cell lines tested in a concentration dependent manner. The potent anticancer activity was observed with the pigment with IC50 values of 26 Î¼g/mL on HeLa and 31 Î¼g/mL on A549 cells, respectively.
Conclusion: The study is pioneering report for determining the better in vitro anticancer activity of violacein from the novel isolate C. vaccinii CV5.
4. Aranda S, Montes-Borrego M, Landa BB. Purple-pigmented violacein-producing Duganella spp. inhabit the rhizosphere of wild and cultivated olives in southern Spain. Microb Ecol 2011;62(2):446-59.
5. Pantanella F, Berlutti F, Passariello C, Sarli S, Morea C, Schippa S. Violacein and biofilm production in Janthinobacterium lividum. J Appl Microbiol 2007;102:992-9.
6. Lu Y, Wang L, Xue Y, Zhang C, Xing XH, Lou K, et al. Production of violet pigment by a newly isolated psychotropic bacterium from a glacier in Xinjiang, China. Biochem Eng J 2009;43:135-41.
7. Matz C, Webb JS, Schupp PJ, Phang SY, Penesyan A, Egan S, et al. Marine biofilm bacteria evade eukaryotic predation by targeted chemical defense. PLoS One 2008;3(4):e2744.
8. Yang LH, Xiong H, Lee OO, Qi SH, Qian PY. Effect of agitation on violacein production in Pseudoalteromonas luteoviolacea isolated from a marine sponge. Lett Appl Microbiol 2007;44(6):625-30.
9. Xu H, Yao L, Sung H, Wu L. Chemical composition and antitumor activity of different polysaccharides from the roots Actinidia eriantha. Carbohydr Polym 2009;78:316-22.
10. Ravin S, Gourav C, Anterpret C, Reena VS. Antioxidant and anticancer activity of methanolic extract from Stephania elegans. Int J Pharm Pharm Sci 2017;9(2):245-9.
11. Felisa P, Nisha K, Hyacinth H, Linz BG. In vitro antioxidant and anticancer activity of Mimosa pudica Linn extract and L-mimosine on lymphoma daudi cells. Int J Pharm Pharm Sci 2015;12:100-4.
12. Kaefer CM, Milner JA. The role of herbs and spices in cancer prevention. J Nutr Biochem 2008;19(6):347-61.
13. Vishnu TS, Palaniswamy M. Isolation and identification of Chromobacterium sp from different ecosystems. Asian J Pharm Clin Res 2016;9(3):253-7.
14. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55-63.
15. Kodach LL, Bos CL, DurÃ¡n N, Peppelenbosch MP, Ferreira CV, Hardwick JC. Violacein synergistically increases 5-fluorouracil cytotoxicity, induces apoptosis and inhibits Akt-mediated signal transduction in human colorectal cancer cells. Carcinogenesis 2006;27(3):508-16.
16. Ferreira CV, Bos CL, Versteeg HH, Justo GZ, DurÃ¡n N, Peppelenbosch MP. Molecular mechanism of violacein-mediated human leukemia cell death. Blood 2004;104(5):1459-64.
17. Melo PS, Justo GZ, de Azevedo MB, DurÃ¡n N, Haun M. Violacein and its beta-cyclodextrin complexes induce apoptosis and differentiation in HL60 cells. Toxicology 2003;186(3):217-25.
18. Saraiva VS, Marshall JC, Cools-Lartigue J, Burnier MN Jr. Cytotoxic effects of violacein in human uveal melanoma cell lines. Melanoma Res 2004;14(5):421-4.
19. Yao G, Yang L, Hu Y, Liang J, Liang J, Hou Y. Nonylphenol-induced thymocyte apoptosis involved caspase-3 activation and mitochondrial depolarization. Mol Immunol 2006;43:915-26.
20. Walsh JG, Cullen SP, Sheridan C, LÃ¼thi AU, Gerner C, Martin SJ. Executioner caspase-3 and caspase-7 are functionally distinct proteases. Proc Natl Acad Sci U S A 2008;105(35):12815-9.
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