PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID


Omer H Ahmed, Maha N Hamad, Noor S Jaafar

Abstract


 

 Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.

Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.

Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.

Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.


Keywords


Chenopodium murale, Coumarins, Phenolic acid, High-performance liquid chromatography, High-performance thin-layer chromatography

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About this article

Title

PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID

Keywords

Chenopodium murale, Coumarins, Phenolic acid, High-performance liquid chromatography, High-performance thin-layer chromatography

DOI

10.22159/ajpcr.2017.v10i11.20504

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 70-77

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

26 Views | 66 Downloads

Authors & Affiliations

Omer H Ahmed
Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Tikrit, Tikrit, Iraq.
Iraq

Maha N Hamad
Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Iraq

Noor S Jaafar
Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Iraq


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