• Krishnavignesh L Department of Embryology, Chennai Fertility Center and Research Institute, Chennai, Tamil Nadu, India.
  • Mahalakshmipriya A Department of Botany, Government Arts College, Coimbatore, Tamil Nadu, India
  • Ramesh M Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore, Tamil Nadu,



 Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.

Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.

Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.

Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

Keywords: Wedelia glauca, Urinary tract infections, Antimicrobial resistance, Antibacterial, Antifungal, Soxhalation, Phytochemical analysis, Chromatography-mass spectrometry.

Author Biographies

Krishnavignesh L, Department of Embryology, Chennai Fertility Center and Research Institute, Chennai, Tamil Nadu, India.

Department of Embryology

Clinical Research Associate

Mahalakshmipriya A, Department of Botany, Government Arts College, Coimbatore, Tamil Nadu, India

Department of Botany

Assistant Professor


Ramesh M, Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore, Tamil Nadu,




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How to Cite
Krishnavignesh L, M. A, and R. M. “GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 11, Nov. 2017, pp. 109-14, doi:10.22159/ajpcr.2017.v10i11.20557.
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