GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN


Krishnavignesh L Krishnavignesh, Mahalakshmipriya A, Ramesh M

Abstract


 

 Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.

Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.

Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.

Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.


Keywords


Wedelia glauca, Urinary tract infections, Antimicrobial resistance, Antibacterial, Antifungal, Soxhalation, Phytochemical analysis, Chromatography-mass spectrometry.

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About this article

Title

GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

Topics

BIOTECHNOLOGY

Keywords

Wedelia glauca, Urinary tract infections, Antimicrobial resistance, Antibacterial, Antifungal, Soxhalation, Phytochemical analysis, Chromatography-mass spectrometry.

DOI

10.22159/ajpcr.2017.v10i11.20557

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 109-114

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

18 Views | 47 Downloads

Authors & Affiliations

Krishnavignesh L Krishnavignesh
Department of Embryology, Chennai Fertility Center and Research Institute, Chennai, Tamil Nadu, India.
India

Mahalakshmipriya A
Department of Botany, Government Arts College, Coimbatore, Tamil Nadu, India
India

Ramesh M
Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore, Tamil Nadu,
India


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