ACUTE TOXICITY TESTING OF NEWLY DISCOVERED POTENTIAL ANTIHEPATITIS B VIRUS AGENTS OF PLANT ORIGIN


Subaiea Gm, Aljofan M, Devadasu Vr, Alshammari Tm

Abstract


Objective: Our previous studies indicate that alkaloids could be developed as potential antihepatitis B agents. In the present study, we investigated the in vitro antihepatitis B virus (HBV) activity and in vivo acute oral toxicity of three isoquinoline alkaloids [-(-) Canadine, Corydadine, and Berberine] obtained from Fumaria and Corydalis species. The compounds were selected based on their therapeutic indexes calculated previously in vitro.

Methods: The antiviral activity and cytotoxicity of selected isoquinoline alkaloids were evaluated in vitro in HepG2 cells. In vivo, acute oral toxicity was performed in female mice following the Organization for Economic Cooperation and Development test guideline-423 (acute toxicity class method).

Results: The selected agents have shown high antiviral activity against HBV and low cytotoxicity in vitro. The results obtained from an acute oral toxicity study revealed that the LD50 of all the test compounds was >2000 mg/kg when administered orally to mice. All the tested compounds fall under the category 5 (unclassified) according to the Globally Harmonized System, with a LD50 value >2000 mg/kg when orally administered to mice.

Conclusion: The results of the study revealed that OR-13 and MNAD can be studied further and can be developed as antihepatitis B drugs.


Keywords


Hepatitis B virus, Isoquinoline alkaloids, Antiviral, Cytotoxicity, Acute toxic class method, Organization for Economic Cooperation and Development 423.

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About this article

Title

ACUTE TOXICITY TESTING OF NEWLY DISCOVERED POTENTIAL ANTIHEPATITIS B VIRUS AGENTS OF PLANT ORIGIN

Topics

Preclinical Drug Discovery

Keywords

Hepatitis B virus, Isoquinoline alkaloids, Antiviral, Cytotoxicity, Acute toxic class method, Organization for Economic Cooperation and Development 423.

DOI

10.22159/ajpcr.2017.v10i11.20717

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 210-213

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

32 Views | 55 Downloads

Authors & Affiliations

Subaiea Gm
Department of Pharmacology and Toxicology, College of Pharmacy, University of Hail, Kingdom of Saudi Arabia.
Saudi Arabia

Aljofan M
Department of Clinical Pharmacy, College of Pharmacy, University of Hail, Kingdom of Saudi Arabia.
Saudi Arabia

Devadasu Vr
Department of Clinical Pharmacy, College of Pharmacy, University of Hail, Kingdom of Saudi Arabia.
Saudi Arabia

Alshammari Tm
Department of Clinical Pharmacy, College of Pharmacy, University of Hail, Kingdom of Saudi Arabia.
Saudi Arabia


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