I Wayan Weta, Mahadewa Tjokorda Gb, Wayan P Sutirtayasa, Anak Agung Ngurah Subawa, Safarina G Malik, I Putu Eka Widyadharma



 Objectives: In addition to the rise in obesity prevalence globally, morbidity due to nonalcoholic fatty liver disease is increasing. Primary modalities for preventing and managing this problem include dietary modification and improved physical activities. A daily diet with a low n-6:n-3 polyunsaturated fatty acid (PUFA) ratio is suspected to contribute to ameliorating liver steatosis (LS). The present study was conducted to elucidate the effects of an n-6:n-3 PUFA ratio of 2:1 in alleviating LS.

Methods: Twenty-four young obese women with LS were recruited from Denpasar, Bali, Indonesia. They were randomly allocated to an intervention or control group. Both groups were given linoleic acid:α-linolenic acid at ratios of 2035:970 and 240:100 g, respectively, for 12 weeks. Baseline and end-line data were obtained. All patients were advised to maintain their daily energy intake no more than 1500 kcal and to perform structured physical exercises once a week.

Results: The intervention significantly decreased the body fat (body mass index, p=0.040; triglyceride, p=0.008) and serum tumor necrosis factor-α (TNF-α) levels (p=0.002) and increased serum interleukin-10 (IL-10) levels (p=0.004). The severity of LS was reduced through the intervention (odds ratio=0.064; 95% confidence interval=0.013-0.310; p=0.001).

Conclusion: An increased intake of 2:1 n-6:n-3 PUFA ratio alleviated LS, decreased body fat composition and serum TNF-α levels, and increased serum IL-10 levels.


young obese women, 2:1 n-6:n-3 PUFA ratio, cytokines, liver steatosis

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Biomedical science; Nurition


young obese women, 2:1 n-6:n-3 PUFA ratio, cytokines, liver steatosis





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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 74-79

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Authors & Affiliations

I Wayan Weta
Department of Community and Preventive Medicine, Clinical Nutrition, Faculty of Medicine, Udayana University, Sanglah Hospital, Denpasar, Bali, Indonesia.

Mahadewa Tjokorda Gb
Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia

Wayan P Sutirtayasa
Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia

Anak Agung Ngurah Subawa
Department of Clinical Pathology, Faculty of Medicine, Sanglah Hospital, Denpasar, Bali, Indonesia.

Safarina G Malik
Eijkman Institute for Molecular Biology, Ministry of Research Technology and Higher Education, Republic of Indonesia, Jakarta, Indonesia.

I Putu Eka Widyadharma
Faculty of Medicine, Udayana University, Denpasar, Bali, Indonesia

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