ANTIOXIDANT AND ANTICANCER POTENTIALS OF BIOFABRICATED SILVER NANOPARTICLES


Merina Paul Das, L Jeyanthi Rebecca

Abstract


 

 Objective: The objective of this study is to explore a rapid, bio-inspired approach to synthesize silver nanoparticles (AgNPs) using aqueous Nardostachys jatamansi leaf extract and evaluate its antioxidant and cytotoxic activities on human colon carcinoma (HCT-116) cell lines.

Methods: The biosynthesized nanoparticles were analyzed using ultraviolet-visible spectrophotometer, scanning electron microscope (SEM), energy dispersive X-ray, X-ray diffractometer (XRD), and Fourier transform infrared spectroscopy. Free radical scavenging and cytotoxic studies were carried out at different concentrations of AgNPs (20-100 μg/mL) using antioxidant 2,2-diphenyl-1-picrylhydrazil (DPPH) and mitochondrial function assay methods.

Results: Surface plasmon resonance spectrum at 434 nm confirmed the formation of AgNPs. SEM images show biosynthesized AgNPs are mostly spherical shaped within the range of 30.0-58.7 nm. XRD analysis reveals the crystallographic face-centered cubic structure of the AgNPs. Thus, synthesized metal nanoparticles were tested for antioxidant activity by DPPH assay, and anticancer activity was validated by lactate dehydrogenase leakage assay. Significant antioxidant property was observed as compared to standard L-ascorbic acid. Further, AgNPs showed a linear dose-response relationship against HCT-116 cell lines with increasing concentration of AgNPs. At a concentration of 20 μg/mL, AgNPs were able to inhibit the cell line’s growth by less than 9.8 ± 0.7%, whereas 100 μg/mL of AgNPs significantly inhibited the cell line’s growth greater than 90.4 ± 0.25%.

Conclusion: The synthesized AgNPs were found to be highly stable and had significant antioxidant and anticancer activity against HCT-116 cell lines. It has wide applications in the biomedical field and can be produced with eco-friendly, rapid scale-up, and easy downstream processing.


Keywords


Nardostachys jatamansi, Approach to synthesize silver nanoparticles, Characterization, Antioxidant, Cytotoxicity.

| PDF |

References


Smith AM, Duan H, Rhyner MN, Ruan G, Nie SA. Synthesis of gold nanoparticles bearing the bio conjugation. Phys Chem Chem Phys 2006;8:3895-903.

Kearns GJ, Foster EW, Hutchison JE. Substrates for direct imaging of chemically functionalized SiO2 surfaces by transmission electron microscopy. Anal Chem 2006;78(1):298-303.

Sarkar S, Jana AD, Samanta SK, Mostafa G. Facile synthesis of silver nanoparticles with highly efficient anti-microbial property. Polyhedron 2007;26:4419-26.

Yu DG. Formation of colloidal silver nanoparticles stabilized by Na+-poly (gamma-glutamic acid)-silver nitrate complex via chemical reduction process. Colloids Surf B Biointerfaces 2007;59(2):171-8.

Tan Y, Wang Y, Jiang L, Zhu D. Thiosalicylic acid-functionalized silver nanoparticles synthesized in one-phase system. J Colloid Interface Sci 2002;249(2):336-45.

Petit C, Lixon P, Pileni MP. In situ synthesis of silver nanocluster in AOT reverse micelles. J Phys Chem 1993;97(49):12974-83.

Vorobyova SA, Lesnikovich AI, Sobal NS. Preparation of silver nanoparticles by interphase reduction. Colloids Surf A 1999;152:375-9.

Bae CH, Nam SH, Park SM. Aluminium nanoparticles production by laser ablation in liquids. Appl Surf Sci 2002;197:628-34.

Smetana AB, Klabunde KJ, Sorensen CM Synthesis of spherical silver nanoparticles by digestive ripening, stabilization with various agents, and their 3-D and 2-D superlattice formation. J Colloid Interface Sci 2005;284(2):521-6.

Mallick K, Witcombb MJ, Scurrella MS. Self-assembly of silver nanoparticles in a polymer solvent: Formation of a nano chain through nano scale soldering. Mater Chem Phys 2005;90:221-4.

Liu YC, Lin LH. New pathway for the synthesis of ultrafine silver nanoparticles from bulk silver substrates in aqueous solutions by son electrochemical methods. Electrochem Commun 2004;6(11):1163-8.

Sandmann G, Dietz H, Plieth W. Preparation of silver nanoparticles on ITO surfaces by a double-pulse method. Electroanal Chem 2000;491(1-2):78-86.

Saxena A, Tripathi RM, Singh RP. Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Dig J Nanomater Bios 2010;5(2):427-32.

Rajakumar G, Rahuman AA. Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta Trop 2011;118(3):196-203.

Sharma D, Kanchi S, Bisetty K. Biogenic synthesis of nanoparticles: A review. Arabian J Chem 2015;7:1131-9.

Vishnu AV, Subhash N, Shakilabanu A, Kurian GA. Characterization and biological evaluation of silver nanoparticles synthesized by aqueous root extract of Desmodium gangeticum for its antioxidant, antimicrobial and cytotoxicity. Int J Pharm Pharm Sci 2014;7 Suppl 1:182-6.

Das MP, Rebecca JL, Veluswamy P, Das J. Exploration of Wedelia chinensis leaf-assisted silver nanoparticles for antioxidant, antibacterial and in vitro cytotoxic applications. J Food Drug Anal 2017;157:1801-8.

Kalaiyarasu T, Karthi N, Gowri VS, Manju V. In vitro assessment of antioxidant and antibacterial activity of green synthesized silver nanoparticles from Digitaria radicosa leaves. Asian J Pharm Clin Res 2016;9(1):297-302.

Subha V, Ravindran RS, Hariram J, Renganathan S. Bioreduction of silver nanoparticles from aqueous stem extract of Catharanthus roseus and bactericidal effects. Asian J Pharm Clin Res 2015;8(5):115-8.

Rastogi L, Arunachalam J. Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nanoparticles using aqueous garlic (Allium sativum) extract and their antibacterial potential. Mater Chem Phys 2011;129:558-63.

Rekha K, Rao RR, Pandey R, Prasad KR, Babu KS, Vangala JR, et al. Two new sesquiterpenoids from the rhizomes of Nardostachys jatamansi. J Asian Nat Prod Res 2013;15(2):111-6.

Singh A. Herbalism, Photochemistry and Ethno Pharmacology. Boca Raton: CRC Press; 2011.

Khan MB, Hoda MN, Ishrat T, Ahmad S, Moshahid Khan M, Ahmad A, et al. Neuroprotective efficacy of Nardostachys jatamansi and crocetin in conjunction with selenium in cognitive impairment. Neurol Sci 2012;33(5):1011-20.

Chaudhary S, Chandrashekar KS, Pai KS, Setty MM, Devkar RA, Reddy ND, et al. Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma. BMC Complement Altern Med 2015;15:50.

Purnima MB, Kothiyal P. A review article on photochemistry and pharmacological profiles of Nardostachys jatamansi DC medicinal herb. J Pharmacogn Phytochem 2015;3(5):102-6.

Hu F, Lu R, Huang B, Liang M. Free radical scavenging activity of extracts prepared from fresh leaves of selected Chinese medicinal plants. Fitoterapia 2004;75(1):14-23.

Vivek R, Kannan S, Achiraman S, Thirumurugan R, Ganesh DS, Krishnan M. Survivin deficiency leads to imparalization of cytokinesis in cancer cells. Asian Pac J Cancer Prev 2011;12(7):1675-9.

Kirthika P, Dheeba B, Sivakumar R, Abdulla SS. Plant mediated synthesis and characterization of silver nanoparticles. Int J Pharm Pharm Sci 2014;6:304-10.

Ramalingam V, Rajaram R, Kumar CP, Santhanam P, Dhinesh P, Vinothkumar S, et al. Biosynthesis of silver nanoparticles from deep sea bacterium Pseudomonas aeruginosa JQ989348 for antimicrobial, ant biofilm, and cytotoxic activity. J Basic Microbiol 2014;54(9):928-36.

Muthukrishnan S, Bhakya S, Kumar TS, Rao MV. Biosynthesis, characterization and antibacterial effect of plant-mediated silver nanoparticles using Ceropegia thwaitesii-an endemic species. Ind Crops Prod 2015;63:119-24.

Wang ZM, He XX, Sun DQ. Practical Infrared Spectroscopy. Beijing: Petroleum Industry Press; 1982. p. 164-6.

Wilson A, Prabukumar S, Sathishkumar G, Sivaramakrishnan S. Aspergillus flavus mediated silver nanoparticles synthesis and evaluation of its antimicrobial activity against different human pathogens. Int J App Pharm 2016;8(4):43-6.

Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol Res Health 2003;27(4):277-84.

Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Appl Nanosci 2016;6:755-66.

Gogoi SK, Gopinath P, Paul A, Ramesh A, Ghosh SS, Chattopadhyay A. Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir 2006;22(22):9322-8.

Suganya G, Karthi S, Shivakumar MS. Larvicidal potential of silver nanoparticles synthesized from Leucas aspera leaf extracts against dengue vector Aedes aegypti. Parasitol Res 2014;113(3):875-80.




About this article

Title

ANTIOXIDANT AND ANTICANCER POTENTIALS OF BIOFABRICATED SILVER NANOPARTICLES

Keywords

Nardostachys jatamansi, Approach to synthesize silver nanoparticles, Characterization, Antioxidant, Cytotoxicity.

DOI

10.22159/ajpcr.2017.v10i12.20878

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 305-308

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

9 Views | 33 Downloads

Authors & Affiliations

Merina Paul Das
Department of Industrial Biotechnology, Bharath University, Chennai, Tamil Nadu, India.
India

L Jeyanthi Rebecca
Department of Industrial Biotechnology, Bharath University, Chennai, Tamil Nadu, India.
India


Article Tools


Email this article (Login required)
Email the author (Login required)

Refbacks

  • There are currently no refbacks.