VANILLIN EXTRACTED FROM PROSO MILLET AND BARNYARD MILLET INDUCE APOPTOSIS IN HT-29 AND MCF-7 CELL LINE THROUGH MITOCHONDRIA MEDIATED PATHWAY


Deepa Priya Ramadoss, Nageswaran Sivalingam

Abstract


Objective: The main aim of the study was to investigate the bioactive compound vanillin extracted from proso millet (compound 1), and barnyard millet (compound 2) induces apoptotic cell death and whether it is mediated through mitochondrial pathway in HT-29 and MCF-7 cell line.

Methods: The cells were treated with 250 μg/ml and 1000 μg/ml concentration of extracted vanillin for 48 hrs. Cytochrome c release and expression level of pro-apoptotic protein Bax and caspase-9 were detected by western blot analysis.

Results: The results reveal that extracted compounds increased the release of cytochrome c and upregulating the expression of Bax and caspase-9 as concentration increases in a dose-dependent manner.

Conclusion: The study suggests that the vanillin compound extracted from these millets induces apoptotic cell death through a mitochondria-dependent pathway.


Keywords


Proso millet, Barnyard millet, Vanillin, HT-29 cells, MCF-7 cells, Cytochrome c, Bax, Caspase-9.

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About this article

Title

VANILLIN EXTRACTED FROM PROSO MILLET AND BARNYARD MILLET INDUCE APOPTOSIS IN HT-29 AND MCF-7 CELL LINE THROUGH MITOCHONDRIA MEDIATED PATHWAY

Keywords

Proso millet, Barnyard millet, Vanillin, HT-29 cells, MCF-7 cells, Cytochrome c, Bax, Caspase-9.

DOI

10.22159/ajpcr.2017.v10i12.21088

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 226-229

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

15 Views | 32 Downloads

Authors & Affiliations

Deepa Priya Ramadoss
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Kan
India

Nageswaran Sivalingam
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Kan
India


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