DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTOR METHOD TO ANALYSIS OF KAEMPFEROL MARKER FROM EXTRACTS OF POINCIANELLA PYRAMIDALIS (TUL) L.P QUEIROZ


Agna Heliade Oliveira, Valmir Gomesde Souza, FÁbio Santosde Souza, Rui Oliveira Macedo

Abstract


Objective: This study aims to develop the extraction of the marker kaempferol in the fluid extract (FE) and validate an analytical method that monitors the quality of extracts of P. pyramidalis.

Methods: The P. pyramidalis leaves were collected and then were dried to milling process. The extracts were drawn up at 20% weight: Volume (w/v) by maceration, and the extraction system used was hydroethanol solution ratio at 50:50 volume: Volume (v: v). From the hydroalcoholic extract, a method of extracting the kaempferol biomarker was developed and validated by high-performance liquid chromatography coupled with diode array detector. To validate a method, the following parameters were evaluated: Specificity, selectivity, linearity, limit of quantification (LOQ) and detection (LOD), precision, accuracy, robustness, and stability.

Results: The method developed proved to be efficient, as it allowed the analysis of the interferents free marker, with recovery above 90%, linear over the range 1.4–26.6 μg/mL, correlation coefficient R2=0.999, and LOD and LOQ 0.07 and 0.22 μg/mL, respectively, specificity, precision, accuracy, and robustness.

Conclusion: The extraction methodology of the kaempferol marker was successfully developed interferents free and the validated method by HPLC-DAD represents a useful tool in the quality control of P. pyramidalis herbal medicines.


Keywords


Analytical validation method, Poincianella pyramidalis, Kaempferol, HPLC-DAD.

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About this article

Title

DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTOR METHOD TO ANALYSIS OF KAEMPFEROL MARKER FROM EXTRACTS OF POINCIANELLA PYRAMIDALIS (TUL) L.P QUEIROZ

Topics

Pharmacy, Analytical chemistry, phytochemistry

Keywords

Analytical validation method, Poincianella pyramidalis, Kaempferol, HPLC-DAD.

DOI

10.22159/ajpcr.2017.v10i12.21321

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 252-259

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

13 Views | 11 Downloads

Authors & Affiliations

Agna Heliade Oliveira
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazil
Brazil

Valmir Gomesde Souza
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazil
Brazil

FÁbio Santosde Souza
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazil
Brazil

Rui Oliveira Macedo
Department of Pharmaceutical Sciences, Medicine Development and Assays Unified Laboratories, Federal University of Paraíba, João Pessoa, PB, Brazil
Brazil


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