• K KUSMARDI Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • A ESTUNINGTYAS Department of Pharmacology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • D SHAVERA Biomedical Science Magister Program, Faculty of Medicine, Universitas Indonesia, Jakarta
  • A TEDJO Department of Chemical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta
  • BP PRIOSOERYANTO Department of Pathology, Faculty of Veteriner, Bogor Agricultural Institute, Bogor



Pericarp, Phaleria macrocarpa, Inflammatory cells, Inducible nitric oxide synthase, Dextran sodium sulfate, Colon



 Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS).

Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.

Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24.

Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS.



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How to Cite

KUSMARDI, K., A. ESTUNINGTYAS, D. . SHAVERA, A. . TEDJO, and B. . PRIOSOERYANTO. “THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 12, Dec. 2017, pp. 309-12, doi:10.22159/ajpcr.2017.v10i12.20560.



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