EFFECTS OF THEAFLAVINS ON GASTRIC CANCER CELL LINE


Surmi Roy, Arpita Saha, Ananya Chatterjee, Sirshendu Chatterjee, Santasree Mazumder, Sandip K Bandyopadhyay

Abstract


 

 Objective: The objective of this study was to evaluate the anticarcinogenic effects of theaflavins (TFs), component of black tea, on human gastric cancer cell line.

Materials and Methods: Cell viability was assessed by WST-1 reagent. Acridine orange (AO) and ethidium bromide (EB) fluorescence staining method were used to detect apoptotic cells, if any, followed by flow cytometric detection. To evaluate the molecular basis for understanding the apoptotic pathway, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were measured. Pro- and anti-apoptotic protein and gene expressions were detected by western blot and real-time techniques.

Results: TFs-treated AGS cell indicated apoptosis-mediated cell death. The presence of TFs induced the generation of ROS and which in turn changes the MMP with the elevated level of cyt c, activation of caspase-9, caspase-3, and PARP cleavage. Our study also showed that TFs treatment altered the Bax/Bcl2 ratio and upregulated the expression of p53.

Conclusion: All these observations lead to the fact that TFs developed potential chemopreventive effects through the induction of apoptosis through mitochondria-mediated death cascade in the human gastric cancer cell.


Keywords


AGS, Apoptosis, Bax, Bcl‑2, Mitochondrial membrane potential, Reactive oxygen species, Theaflavins.

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About this article

Title

EFFECTS OF THEAFLAVINS ON GASTRIC CANCER CELL LINE

Topics

Cancer Biology

Keywords

AGS, Apoptosis, Bax, Bcl‑2, Mitochondrial membrane potential, Reactive oxygen species, Theaflavins.

DOI

10.22159/ajpcr.2018.v11i1.21606

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 294-298

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

107 Views | 39 Downloads

Authors & Affiliations

Surmi Roy
Department of Biochemistry, KPC Medical College and Hospital, Kolkata, West Bengal, India.
India

Arpita Saha
Department of Biochemistry, KPC Medical College and Hospital, Kolkata, West Bengal, India.
India

Ananya Chatterjee
Department of Biochemistry, KPC Medical College and Hospital, Kolkata, West Bengal, India.
India

Sirshendu Chatterjee
Department of Biotechnology, Techno India University
India

Santasree Mazumder
Department of Biochemistry, University of Calcutta, Kolkata, West Bengal, India.
India

Sandip K Bandyopadhyay
Department of Biochemistry, KPC Medical College and Hospital, Kolkata, West Bengal, India.
India


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