IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM LEAVES ON EXTENDED-SPECTRUM BETA-LACTAMASE AND AMBLER CLASS C COPRODUCERS FROM SKIN AND SOFT-TISSUE INFECTIONS.


Pratibha J Shah, Manita T Williamson

Abstract


 Objective: Clinical isolates coproducing extended-spectrum beta-lactamase (ESBL) and Ambler Class C (AmpC) beta-lactamase are a therapeutic challenge. The aim of the present study was to evaluate the antibacterial activity of Azadirachta indica methanolic leaf extract against lactose fermenting coproducers of ESBL and AmpC isolated from skin and soft-tissue infections (SSTIs).

Methods: A total of 88 non-duplicate lactose fermenting strains isolated from SSTIs specimens were tested for their antibiotic susceptibility pattern by Kirby-Bauer disk diffusion method. Ceftazidime and cefoxitin-resistant strains were screened for coproduction of ESBL and AmpC beta-lactamase by phenotypic confirmatory disc diffusion test and E-test. Antibacterial activity of methanolic neem leaf (NLM) extract was examined by the disc diffusion method and the minimum inhibitory concentration (MIC) of NLM was determined by the agar dilution technique.

Results: Thirty-seven lactose fermenting strains were confirmed to be coproducers of ESBL and AmpC, out of which 27 were Escherichia coli and 10 were Klebsiella spp. The nimbin content in the neem leaf extract was found to be 0.007% (w/w) by high-performance liquid chromatography (HPLC) analysis. The NLM extract showed inhibitory activity against all the 37 lactose fermenting isolates from SSTIs, coproducing ESBL and AmpC. The MIC of NLM extract was determined to be in the range of 4–8% (40–80 mg/ml) against the selected isolates.

Conclusion: It can be stated that NLM extract might have therapeutic significance against coproducers of ESBL and AmpC beta-lactamase isolated from SSTIs.


Keywords


Extended-spectrum beta-lactamase, Ambler Class C, Azadirachta indica, Skin and soft-tissue infections, E-test, High-performance liquid chromatography.

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About this article

Title

IN VITRO ANTIBACTERIAL ACTIVITY OF NEEM LEAVES ON EXTENDED-SPECTRUM BETA-LACTAMASE AND AMBLER CLASS C COPRODUCERS FROM SKIN AND SOFT-TISSUE INFECTIONS.

Topics

clinical research

Keywords

Extended-spectrum beta-lactamase, Ambler Class C, Azadirachta indica, Skin and soft-tissue infections, E-test, High-performance liquid chromatography.

DOI

10.22159/ajpcr.2018.v11i1.21754

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 210-213

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Pratibha J Shah
Department of Microbiology, K.C. College, Churchgate, Mumbai, Maharashtra, India.
India

Manita T Williamson
Department of Microbiology, T.N.M.C & B.Y.L. Nair Charitable Hospital, Mumbai, Maharashtra, India.
India


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