AMELIORATIVE EFFECTS OF MELATONIN AGAINST 2,4-DICHLOROPHENOXYACETIC ACID TOXICITY IN KIDNEY OF MICE – A HISTOLOGICAL STUDY.

  • Ashlesh M Upadhyaya Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University,Ahmedabad - 380 009, Gujarat, India.
  • Mandava V Rao Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad – 380009, Gujarat, India.
  • Devendrasinh D Jhala Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University,Ahmedabad - 380 009, Gujarat, India.

Abstract

 Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.

Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II - melatonin alone (10 mg/kg body weight); Group III - 2,4-D alone (50 mg/kg body weight); and Group IV - 2,4-D + melatonin was given orally for 45 days.

Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules.

Conclusion: Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity.

 

Keywords: 2, 4-dichlorophenoxyacetic acid, Histopathology, Melatonin, Mice, Mitigation, Nephrotoxicity.

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Upadhyaya, A. M., M. V. Rao, and D. D. Jhala. “AMELIORATIVE EFFECTS OF MELATONIN AGAINST 2,4-DICHLOROPHENOXYACETIC ACID TOXICITY IN KIDNEY OF MICE – A HISTOLOGICAL STUDY.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 1, Jan. 2018, pp. 78-82, doi:10.22159/ajpcr.2018.v11i1.21829.
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