AMELIORATIVE EFFECTS OF MELATONIN AGAINST 2,4-DICHLOROPHENOXYACETIC ACID TOXICITY IN KIDNEY OF MICE – A HISTOLOGICAL STUDY.


Ashlesh M Upadhyaya, Mandava V Rao, Devendrasinh D Jhala

Abstract


 Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.

Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II - melatonin alone (10 mg/kg body weight); Group III - 2,4-D alone (50 mg/kg body weight); and Group IV - 2,4-D + melatonin was given orally for 45 days.

Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules.

Conclusion: Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity.

 


Keywords


2,4-dichlorophenoxyacetic acid, Histopathology, Melatonin, Mice, Mitigation, Nephrotoxicity.

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About this article

Title

AMELIORATIVE EFFECTS OF MELATONIN AGAINST 2,4-DICHLOROPHENOXYACETIC ACID TOXICITY IN KIDNEY OF MICE – A HISTOLOGICAL STUDY.

Keywords

2,4-dichlorophenoxyacetic acid, Histopathology, Melatonin, Mice, Mitigation, Nephrotoxicity.

DOI

10.22159/ajpcr.2018.v11i1.21829

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 78-82

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Ashlesh M Upadhyaya
Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University,Ahmedabad - 380 009, Gujarat, India.
India

Mandava V Rao
Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University, Ahmedabad – 380009, Gujarat, India.
India

Devendrasinh D Jhala
Department of Zoology, Biomedical Technology and Human Genetics, University School of Sciences, Gujarat University,Ahmedabad - 380 009, Gujarat, India.
India


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