FORMULATION AND EVALUATION OF NOVEL ANTIBACTERIAL AND ANTI-INFLAMMATORY CREAM CONTAINING MUNTINGIA CALABURA LEAVES EXTRACT


Mahendran Sekar, Nur Syahira Abdul Jalil

Abstract


 

 Objective: The main objective of the present study was to formulate antibacterial and anti-inflammatory cream using Muntingia calabura leaves extract.

Methods: 500 g of matured leaves of M. calabura were extracted with 70% methanol using maceration method for a week. The antibacterial activity of the extract and formulated cream in different concentration was investigated against two Gram-positive bacteria (Staphylococcus aureus and Bacillus cereus) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) through disc diffusion method. The anti-inflammatory activity of extract and formulated cream was studied using inhibition of albumin denaturation technique.

Results: The antibacterial activity of the methanolic extract was studied in different concentrations (5, 10, and 25 mg/ml) and the formulated cream was studied in the concentration of (10, 25, 50, and 100 mg/ml). The extract showed significant antibacterial activity with all the tested pathogens with a zone of inhibition ranges from 3.33 ± 0.88-14.60 ± 0.80 mm. As for the formulated cream, the zone of inhibition for F1 ranged from 0.67 ± 0.67 mm to 14.80 ± 1.17 mm. F2 presented zone of inhibition at 3.40 ± 1.64 mm to 15.00 ± 1.18 mm. The anti-inflammatory activity showed maximum inhibition of 63.93 ± 5.90, 41.83 ± 5.64, and 27.20 ± 2.72 was observed at 1000 μg/ml for the extract, F2 and F1, respectively. The cream was found to be in stable state after 2 months.

Conclusion: The results showed that the formulated cream can be used for bacterial infections and to reduce inflammation. We recommend this formulation need to be further developed into commercial standards.


Keywords


Muntingia calabura, Kerukup Siam, Disc diffusion method, Albumin denaturation technique, Antibacterial, Anti-inflammatory.

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About this article

Title

FORMULATION AND EVALUATION OF NOVEL ANTIBACTERIAL AND ANTI-INFLAMMATORY CREAM CONTAINING MUNTINGIA CALABURA LEAVES EXTRACT

Keywords

Muntingia calabura, Kerukup Siam, Disc diffusion method, Albumin denaturation technique, Antibacterial, Anti-inflammatory.

DOI

10.22159/ajpcr.2017.v10i12.21963

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 376-379

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Mahendran Sekar
Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh - 30450, Malaysia.
Malaysia

Nur Syahira Abdul Jalil
Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Health Sciences, Universiti Kuala Lumpur Royal College of Medicine Perak, Ipoh - 30450, Malaysia.
Malaysia


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