DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE


Zahraa Isam, Rabab Omran Al-jelawi-, Ammad Hassan Mahmood

Abstract


 

 Objective: The uromodulin, a glycoprotein, expressed and secreted by epithelial kidney cells lining the thick ascending limb of the Henle’s loop. It is encoded by the UMOD gene in humans. Our objective was to analyze single nucleotide polymorphisms (SNPs) in the UMOD promoter region in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD).

Methods: The blood samples were collected from 100 patients with CKD (50) and ESRD (50), who admitted at Merjan Teaching Hospital in Babylon Province, Iraq (February-July 2016). In addition, 50 blood samples of healthy control. The SNPs of UMOD promoter region was investigated using single-strand conformation polymorphism-polymerase chain polymorphisms (SSCP-PCR) and DNA sequencing techniques.

Results: UOMD promoter region polymorphisms using PCR-SSCP and sequencing DNA appeared three different conformational haplotypes, including A\G 49 haplotype (5 bands), A\G 49 and C\A 247 haplotype (5 bands), and C\G 45 and A\G 49 haplotype (6 bands) distributed among CKD and ESRD cases, due to the presence of three SNPs. There was no association between band numbers of PCR-SSCP with ESRD and CKD compared with a control group.

Conclusion: SSCP-PCR is a good screening method to detect genetic variations in an uromodulin promoter region.


Keywords


Chronic kidney disease, End-stage renal disease, UMOD-promoter region, SNPs, Single-strand conformation polymorphism-polymerase chain polymorphisms.

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References


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About this article

Title

DETECTION SINGLE NUCLEOTIDE POLYMORPHISMS IN UROMODULIN PROMOTER REGION ASSOCIATED WITH RENAL DISEASES USING SINGLE-STRAND CONFORMATION POLYMORPHISM-POLYMERASE CHAIN POLYMORPHISMS TECHNIQUE

Keywords

Chronic kidney disease, End-stage renal disease, UMOD-promoter region, SNPs, Single-strand conformation polymorphism-polymerase chain polymorphisms.

DOI

10.22159/ajpcr.2018.v11i1.22063

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 1 January 2018 Page: 205-209

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

83 Views | 65 Downloads

Authors & Affiliations

Zahraa Isam
Department of Biology, College of Science, University of Babylon, Al-Hillah City, Babel, Iraq.
Iraq

Rabab Omran Al-jelawi-
Department of Biology, College of Science, University of Babylon, Al-Hillah City, Babel, Iraq
Iraq

Ammad Hassan Mahmood
College of Medicine, University of Babylon, Consultant urologist in Hilla Teaching  Hospital, Al-Hillah City, Babel, Iraq.
Iraq


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