ANTIDIABETIC AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC EXTRACT OF PIPER BETLE L. LEAVES IN CATFISH, CLARIAS GARIEPINUS
Â Objective: The present study was undertaken to assess the Î±-amylase inhibitory activity and antidiabetic experimental catfish model and antioxidant properties of Piper betle L. ethanolic (PBE) extract.
Methods: The phytochemical analysis of PBE extract was performed. The PBE extract was tested for their inhibitory effect on the Î±-amylase assay, which compared to the control, acarbose. The absorbance was read at 540 nm using a spectrophotometer, and IC50 values were calculated. In this present investigation, diabetes mellitus was induced in catfish, Clarias gariepinus by epaxial musculature injection to glucose and standard drug, Metformin hydrochloride. After 24-h incubation, the treated fishes were dissected, and the blood, liver, tissue samples, and epaxial musculature regions were collected. In addition, the antioxidant properties of PBE were determined by 2,2-diphenyl-l-picrylhydrazyl (DPPH) radical scavenging and 2,2â€™-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging (ABTS) assays.
Results: The phytochemical screening of PBE revealed the presence of alkaloid, flavonoids, tannins, phenol, glycosides, sterols, saponins, and quinines. Furthermore, the values of (Î¼g/ml) 3.038 and 7.672 Î±-amylase enzyme inhibition were excellent activity when compared to the acarbose. Moreover, elevated the glucose level (mg/dl) was estimated in blood 1.9Â±0.35, liver 0.5Â±0.25, tissue 0.2Â±0.25, and epaxial musculature 0.8Â±0.2 after 24-h incubation. The antioxidant effect of maximum activity was found in PBE; IC50 values (Î¼g/ml) of DPPH and ABTS were 9.362 and 6.606, respectively.
Conclusions: These studies might be responsible for the P. betle L. that was used as the new source of antidiabetic and antioxidant agents.
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