• Reno Keumalazia Kamarlis Department of Pathology Anatomy, Medical Faculty, Syiah Kuala University, Banda Aceh, Indonesia.
  • Muhammad Nadjib Dahlan Lubis Department of Pathology Anatomy, Medical Faculty, Sumatera Utara University, Medan, Indonesia.
  • Bethy S Hernowo Department of Pathology Anatomy, Medical Faculty, Padjajaran University,Bandung, Indonesia.
  • Azmi S Kar Department of Internal Medicine, Medical Faculty, Sumatera Utara University, Medan, Indonesia.
  • Basri A Gani Department of Biology Oral, Dentistry Faculty, Syiah Kuala University, Banda Aceh-Indonesia, Indonesia.


 Objective: To assess the immune expressions of cytoplasmic and nuclear protein63 (p63) proteins in metaplastic triple-negative breast cancer (TNBC) patients with basal-like and non-basal-like types based on age.

Methods: Forty samples of breast cancer patients diagnosed with TNBC were examined immune histochemical based on the immune expressions of cytoplasmic and nuclear p63 with basal-like (CK 5/6, positive) and non-basal-like (CK 5/6 negative) types. The histoscore values were categorized into Score 1 (<20%, negative), Score 2 (>20% - 50%, weak positive), Score 3 (>50% - 80%, moderate positive), and Score 4 (>80 %, strong positive) also analyzed by one-way analysis variance, probability (p<0.05), and correlation (p<0.01).

Results: Forty subjects with breast cancer showed 23 of basal-like (57.5%) and 17 of non-basal-like (42.5%) types. The cytoplasmic p63 had a low histoscores in the group of < 40–49 years old (55%), the moderate group of 40–55 years old (27.5%), and the high group of fewer than 50–60 years old (17.5%). The nuclear p63 was low in all age groups (p<0.05). It was the significant difference between basal-like and non-basal-like types (p<0.01) and strongest correlation (1.00). The immunoexpression of cytoplasmic p63 of basal-like cell type had an intensity of 88.2%, while nuclear p63 possessed 11.8% (p<0.05).

Conclusion: The frequency of cytoplasm p63 in all age groups of metaplastic breast cancer of basal-like type was more dominant than the nuclear p63. Otherwise, in the non-basal-like type, the cytoplasmic p63 protein was lower than the of nuclear p63 protein.

Keywords: Cytoplasmic protein63, Nuclear protein63, Metaplastic breast cancer, Basal-like, Non-basal-like.

Author Biography

Reno Keumalazia Kamarlis, Department of Pathology Anatomy, Medical Faculty, Syiah Kuala University, Banda Aceh, Indonesia.
Department of Oral Biology, Dentistry Faculty, Syiah Kuala University


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How to Cite
Kamarlis, R. K., M. N. Dahlan Lubis, B. S. Hernowo, A. S. Kar, and B. A. Gani. “FREQUENCY AND DISTRIBUTION OF CYTOPLASMIC AND NUCLEAR P63 IN RECOGNIZING THE BASAL-LIKE AND NON-BASAL-LIKE TYPES OF BREAST CANCER BASED ON AGE.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 1, Jan. 2018, pp. 195-9, doi:10.22159/ajpcr.2018.v11i1.22415.
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