EVALUATION OF ANTIOXIDANT, Î‘-GLUCOSIDASE AND Î‘-AMYLASE INHIBITORY ACTIVITIES OF ATALANTIA RACEMOSA AND SENNA UNIFLORA LEAVES
Â Objective: The current investigation was conducted to investigate the total phenol, total flavonoid content, antioxidant, Î±-glucosidase, and Î±-amylase activities in leaves of Atalantia racemosa and Senna uniflora.
Methods: Different crude solvent extracts were prepared and concentrated using rotary evaporator, these solvent extracts were tested to estimate the antioxidant radical scavenging activity using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2, 2â€™-azinobis, 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods using spectrophotometer. The extracts were screened by Î±-amylase inhibition assay and Î±-glucosidase inhibition assay to estimate the antidiabetic potential of the studied plant with the help of microplate reader.
Results: A potent antioxidant activity, i.e. mean percentage inhibition of DPPH and ABTS radical was observed in A. racemosa ethanol (71.5 Â± 0.026%) and ethyl acetate extract (97.3 Â± 0.076%), respectively, at the concentration of 400 Î¼g/mL. Similarly, highest Î±-amylase and Î±-glucosidase inhibitory activities were observed in A. racemosa methanolic (82.4 Â± 0.016%) and ethyl acetate 91.1Â±0.018 extracts, respectively.
Conclusions: The present study revealed that the A. racemosa extracts possessed good antioxidant, Î±-amylase and Î±-glucosidase inhibitory activities; hence, it can be used as a source of natural free radical scavenger and antidiabetic supplement(s). However, further study needs to be carried out to know the active compound and its mode of action.
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