TO EVALUATE THE ANTI-ATHEROSCLEROTIC POTENTIAL OF QUERCETIN IN ALLOXANINDUCED DIABETIC RATS FED WITH HIGH-FAT DIET

  • Biplav S Department of Pharmacology, PES College of Pharmacy, Hanumanthanagar, Bengaluru – 560 050, Karnataka, India
  • Sindhura G Department of Pharmacology, PES College of Pharmacy, Hanumanthanagar, Bengaluru – 560 050, Karnataka, India
  • Shivalinge Gowda K P Department of Pharmacology, PES College of Pharmacy, Hanumanthanagar, Bengaluru – 560 050, Karnataka, India

Abstract

 Objective: The main aim of the present study is concerned with the evaluation of anti-atherosclerotic potential of quercetin in alloxan-induced diabetic rats fed with high-fat diet (HFD).

Methods: Atherosclerosis (AS) is the major cause for many of the cardiovascular disease, and it is accelerated in the presence of diabetes mellitus and causes profound alterations in the lipid profile. The method used for the induction of AS was using HFD for 60 days. In this study, rats were divided into four groups (n=6). Group I served as normal control, Group II alloxan (120 mg/kg b.w i.p)-treated diabetic rats, Group III received quercetin (50 mg/ kg b.w p.o), and Group IV received atorvastatin (10 mg/kg b.w p.o) along with alloxan (120 mg/kg b.w i.p) on the 1st day of the days of the study period. AS was induced in Group II, Group III, and Group IV rats by feeding them with HFD from the 1st day to 60th day. The body weight, feed intake was measured daily. The blood was withdrawn from retro-orbital plexus, and the serum was used for the estimation of lipid profile (total cholesterol [TC], triglycerides [TGs], low-density lipoprotein cholesterol [LDL-C], very LDL-C [VLDL-C], and high-density lipoprotein cholesterol [HDL-C]). After scarification under overdose of ketamine, the histopathological study of aorta was carried out.

Results: The results showed that the quercetin-treated rats showed a decrease in body weight gain, decreased levels of TC, TGs, LDL-C, and VLDL-C, and increased levels of HDL-C were observed in Group III rats when compared to alloxan-induced diabetic rats fed with HFD (Group III). The histopathological study of aorta showed no development of plaques and of foam cells.

Conclusion: From this study, it can be calculated that quercetin has anti-atherosclerotic activity as it significantly altered overall lipid profile in diabetic rats fed with HFD. This activity may be attributed to its antioxidant, inhibition of HMG-CoA reductase activity of quercetin.

Keywords: Alloxan, Quercetin, High-fat diet, Serum lipid profile, Atorvastatin, Atherosclerosis, Diabetes.

References

1. Joshi SC, Jain PK. A review on hypolipidaemic and antioxidant potential of some medicinal plants. World J Pharm Pharm Sci 2014;3:357-80.
2. Suman M, Shivalinge GK, Paul U, Priyanka S. Evaluation of antidiabetic and antihyperlipidemic activity of newly formulated polyherbal antidiabetic tablets in streptozocin-induced diabetes mellitus in rats. Asian J Pharm Clin Res 2016;9:202-7.
3. Martha S, Akula A, Reddy N. Antiatherosclerotic model of atorvastatin and clopidogrel alone and in combination in rats. Indian J Exp Biol 2008;46:698-703.
4. Pamidiboina V, Razdan R, Hariprasad MG. Evaluation of the antihyperlipidemic, cardioprotective activity of a polyherbal formulation. Int J Pharm Pharm Sci2010;2:1-3.
5. Neal RC, Jones PH. Complementary therapy to target LDL cholesterol: The role of the ezetimibe/simvastatin combination. Vasc Health Risk Manag 2006;2:31-8.
6. Seiva FR, Chuffa LG, Braga CP, Amorim JP, Fernandes AA. Quercetin ameliorates glucose and lipid metabolism and improves antioxidant status in postnatally monosodium glutamate-induced metabolic alterations. Food Chem Toxicol 2012;50:3556-61.
7. Rajaram K. Antioxidant and antidiabetic activity of tectonagrandislinn. In alloxan induced albino rats. Asian J Pharm Clin Res 2013;6:174-7.
8. Rajasekaran S, Anandan R, Nishad KM. Anti hyperlipidemic activity of acalyphaindicalinn. On atherogenic diet induced hyperlipidemia. Int J Pharm Pharm Sci 2013;5:699-701.
9. El-Nekeety AA, Abdel-Azeim SH, Hassan AM, Hassan NS, Aly SE, Abdel-Wahhab MA, et al. Quercetin inhibits the cytotoxicity and oxidative stress in liver of rats fed aflatoxin-contaminated diet. Toxicol Rep 2014;1:319-29.
10. Sarvesh CN, Fernandes J. Evaluation of antihyperlipidemic activity of leaves of Achyranthesaspera linn. Using hyperlipidemic rats. Asian J Pharm Clin Res 2017;10:211-5
11. Searcy RL. Diagnostic Biochemistry. New York, NY: McGraw-Hill; 1969.
12. Cole, TG, Klotzsch SG, McNamara J. Measurement of triglyceride concentration. In: Rifai N, Warnick GR, Dominiczak MH, editors. Handbook of Lipoprotein Testing. Washington, DC: AACC Press; 1997. p. 155-26.
13. Burtis CA, Ashwood ER, Bruns DE. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 5th ed. Hudson, OH: WB Saunders Comp; 2012.
14. Fukuyama N, Homma K, Wakana N, Kudo K, Suyama A, Ohazama H, et al. Validation of the friedewald equation for evaluation of plasma LDL-cholesterol. J Clin Biochem Nutr 2008;43:1-5.
15. Mahmoud B, Hetta M, Etwab SA, Hassan S. Improvement of lipid profile and antioxidant hypercholesterolemic albino rats by polysaccharide extracted from the green alga Lactucalinnaeus. Saudi J Biol Sci 2011;18:333-40.
16. Zakaria Z, Das S, Lin TS, Thent CZ. Histological changes in the heart and the proximal aorta in experimental diabetic rats fed with Piper sarmentsou. Afr J Tradit Complement Altern Med 2012;9:396-40.
17. Andallu B, Vinay Kumar AV, Varadacharyulu NCh. Lipid abnormalities in streptozotocin-diabetes: Amelioration by Morus indica L. Cv suguna leaves. Int J Diabetes Dev Ctries 2009;29:123-8.
18. Hoek-van den Hil EF, van Schothorst EM, van der Stelt I, Swarts HJ, Venema D, Sailer M, et al. Quercetin decreases high-fat diet induced body weight gain and accumulation of hepatic and circulating lipids in mice. Genes Nutr 2014;9:418.
19. Kamesh V, Sumathi T. Antihypercholesterolemic effect of Bacopa monniera linn. On high cholesterol diet induced hypercholesterolemia in rats. Asian Pac J Trop Med 2012;5:949-55.
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How to Cite
S, B., S. G, and S. Gowda K P. “TO EVALUATE THE ANTI-ATHEROSCLEROTIC POTENTIAL OF QUERCETIN IN ALLOXANINDUCED DIABETIC RATS FED WITH HIGH-FAT DIET”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 3, Mar. 2018, pp. 379-83, doi:10.22159/ajpcr.2018.v11i3.23056.
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