DETERMINATION OF VOLATILE BIOACTIVE COMPOUNDS FROM EXTRACTS OF BAEL (AEGLE MARMELOS) PLANT PARTS AND THEIR COMPARATIVE ANALYSIS
Â Objective: The main objective of this study is to determine the bioactive compounds from the extracts of wildly growing Aegle marmelos plant parts.
Methods: A. marmelos root, stem, leaves, bark, fruit peel, and pulp were screened for the presence/absence of phytochemicals. Bioactive compounds in all the plant parts were analyzed by gas chromatographyâ€“mass spectrometry (GC/MS) analysis. For evaluation of bioactive compounds first, the column chromatography was done using various solvents and found that the methanolic extracts gave better elution and separation of compounds and hence used further for GC/MS analysis.
Result: GC/MS analysis revealed chromatograms of methanol extract of A. marmelos plant parts, and all the plant parts were found to have a number of phytochemicals. Some compounds, namely, benzene, nitro-, benzenepropanoic acid, 3, 5-bis (1, 1-dimethylethyl)-4-hydroxy-, methyl ester, and tetradecene were found in all parts with a varying percentage. Phenol only found in the fruit of the plant with more percentage in fruit peel (4.38%) than in fruit pulp (0.58%). Dibutyl phthalate is the major compound found in Aegle root (10.43%), fruit peel (34.56%), and pulp (13.18%). Other important compounds such as coumarin (2H-1-Benzopyran-2-one, 7-[(3,7-dimethyl-2,6-octadienyl)oxy]-, (E)-), skimmianine (Furo[2,3-b] quinoline, 4,7,8-trimethoxy-), and cyclobarbital were found in plant root.
Conclusion: After the GC/MS analysis, it was concluded that all the parts of this wildly growing plant contain a significant amount of pharmaceutically important compounds.
2. Bernhoft A. Bioactive Compounds in Plants-Benefits and Risks for Man and Animals. The Norwegian Academy of Science and Letters. Oslo:Proceedings from a Symposium Held at The Norwegian Academy of Science and Letters; 2008.
3. Lim TK. Aegle marmelos. Edible Medicinal and Non-Medicinal Plants: Fruits. 4th ed. London: Springer; 2012. p. 594-615.
4. Lmbole VB, Murti K, Kumar U, Bhatt SP, Gajera V. Phytopharmacological properties of Aegle marmelos as a potential medicinal tree: An Overview. Int J Pharm Sci Rev Res 2010;5:67-72.
5. Upadhyay RK. Bel plant: A source of pharmaceuticals and ethno medicines. Int J Green Pharm 2015;9:205-22.
6. Uma Devi KJ, Vanitha V, Vijayalakshmi K, Tilton F. Determination of bioactive components of Aegle marmelos L. leaves by GC-MS analysis. Indian Streams Res J 2011;1:1-4.
7. Jorge AP, Rolando M, Victor F. Volatile compounds from leaves of Aegle marmelos (L.) Correa grown in Cuba. Rev CENIC Cien QuÃmicas 2005;36:71-3.
8. Satyal P, Woods KE, Dosoky NS, Neupane S, Setzer WN. Biological activities and volatile constituents of Aegle marmelos (L.) Correa from Nepal. J Med Active Plants 2012;1:114-22.
9. Mujeeb F, Bajpai P Pathak N. Phytochemical evaluation, antimicrobial activity and determination of bioactive compounds from leaves of Aegle marmelos. Biomed Res Int 2014;2014:14.
10. Rajeshkannan C, Lakshmanan G. Quantification of chlorogenic acid from aqueous and methanolic extracts of wood apple (Aegle marmelos C. Linn) by GC-MS Ion-trap. Int J Innov Res Sci Eng Technol 2014;3:11087-93.
11. Bajaniya VK, Kandoliya UK, Bodar NH, Bhadja NV, Golakiya BA. Fatty acid profile and phytochemical characterization of Bael seed (Aegle marmelos L.) oil. Int J Curr Microbiol Appl Sci 2015;4:97-102.
12. Nadhiya H, Vadivazhagi KM. Antioxidant and phytochemical properties of combination of leaves of Aegle marmelos and Mentha piperita. Int Res J Pharm Biosci 2015;2:1-9.
13. Jha R, Gupta RK. Development of energy drink containing Aegle marmelos, Rubia cordifolia, Phyllanthus emblica and Beta vulgaris and its phytochemical, nutritive and antimicrobial analysis. J Pharm Phytochem 2016;5:186-93.
14. Kasireddy P, Khumanthem DS, Prashanti P, Manguluri P. Neuroprotective potential and efficacy of neurodegenerative disorders of fruit extract of Aegle marmelos. Int J Pharm Pharm Sci 2015;7:1-5.
15. Yadav SS, Dahiya K, Showkat AG, Gulia SK. Antibacterial activity of Aegle marmelos (L) Correa. Int J Pharm Pharm Sci 2015;7:1-3.
16. Available from: https://www.pubchem.ncbi.nlm.nih.gov.
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