IN VITRO STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS AND THEIR INTERRELATIONSHIP


Shubhi Rastogi, Mohammed Shariq Iqbal, Deepak Ohri

Abstract


 Objective: The objective of the present work is to study the in vitro anti-inflammatory and antioxidant activity of medicinal plants. The extent and correlation between anti-inflammatory and antioxidant activity have been studied.

Method: Methanolic and aqueous extracts of five medicinal plants, namely, Ficus racemosa, Aloe vera, Cannabis sativa, Datura stramonium, and Calotropis gigantean have been taken for in vitro anti-inflammatory and total antioxidant activity.

Result: The study showed that the inhibition of protein (albumin) denaturation was maximum in aqueous extract of A. vera with 97.55±1.45%. Proteinase inhibitory action of different plant extracts showed significant action and was found to be maximum in aqueous extract of D. stramonium with 87.89±2.58%. Heat-induced hemolysis showed that maximum inhibition was with aqueous extract of F. racemosa with 90.72±3.33%. When hypotonicity-induced hemolysis activity was done it was found maximum in methanolic extract of C. gigantea with 90.58±3.04%. Anti-lipoxygenase activity was found maximum in methanolic extract of F. racemosa with 94.05±4.24%. When total antioxidant activity was done, it was found highest in F. racemosa (4.38±0.546 mM equivalent of ascorbic acid/g tissue).

Conclusion: An overall strong positive correlation between anti-inflammatory and antioxidant activity was observed, indicating that antioxidant activity of the plant species studied might be responsible for their anti-inflammatory property. Further work needs to be undertaken to fully elucidate the antioxidants responsible for anti-inflammatory action and to develop better herbal drug formulations.


Keywords


Pharmacology

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References


Poyton RO, Ball KA, Castello PR. Mitochondrial generation of free radicals and hypoxic signaling. Trends Endocrinol Metab 2009;20:332- 40.

Reuter S, Gupta SC, Chaturvedi MM, Aggarwal BB. Oxidative stress, inflammation, and cancer: How are they linked? Free Radic Biol Med 2010;49:1603-16.

Coussens LM, Werb Z. Inflammation and cancer. Nature 2002;420:860- 7.

Hussain SP, Hofseth LJ, Harris CC. Radical causes of cancer. Nat Rev Cancer 2003;3:276-85.

Federico A, Morgillo F, Tuccillo C, Ciardiello F, Loguercio C. Chronic inflammation and oxidative stress in human carcinogenesis. Int J Cancer 2007;121:2381-6.

Hussain SP, Harris CC. Inflammation and cancer: An ancient link with novel potentials. Int J Cancer 2007;121:2373-80.

Tiwari P, Kumar B, Kaur M, Kaur G, Harleen K. Phytochemical screening and Extraction: A Review. Int Pharm Sci 2011;1:98-106.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum preoteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharm Sci 2010;2:146-55.

Oyedepo OO, Femurewa AJ. Anti-protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J Pharmacong 1995;33:65-9.

Sadique J, Al-Rqobahs WA, Bughaith, EI Gindi AR. The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia 1989;60:525-32.

Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, et al. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) loud. Wood oil. Indian J Exp Biol 1999;37:258-61.

Azeem AK, Dilip C, Prasanth SS, Junise V, Hanan S. Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asia Pac J Med 2010;3:412-20.

Cacig S, Szabo MR, Lupea AX, Ardelean A. Determination of the antioxidant activity of Ziziphus jujuba and Hydrangea incognita aqueous extracts. Studia Univ Vasile Goldis Seria St Vietii 2005;15:69- 72.

Iqbal MS, Ansari MI, Jafri S, Padmesh S, Ahmad I, Pandey B. Antioxidant potential of some medicinal plants (Ocimum sanctum, Azadirachta indica and Nigella sativa). Pharmacophore. Int Res J 2014;5:631-7.

Reshma, Arun KP, Brindha P. In vitro anti-inflammatory, antioxidant and nephroprotective studies on leaves of Aegle marmelos and Ocimum sanctum. Asian J Pharm Clin Res 2014;7: 121-9.

Shallangwa GA, Abdul-Hamidu A, Abba H, Dallatu YA, Bilham DT. In-vitro evaluation of aqueous extracts of Citurs sinensis, Aloe vera and their 1:1 extracts blend on protein denaturation during acute inflammation. J Biol Act Prod Nat 2013;3:295-302.

Duraid AA. Analgesiac, anti-inflammatory and antidiarrhoeal effects of Datura stramonium hydroalcoholic leaves extract in mice. Int J Res Rewv Appl Sci 2013;14:193-9.

Das SN, Chatterjee S. Long term toxicity study of ART-400. Indian Ind Med 1995;16:117-23.

Ramadevi M, Sivasubramanian N, Selvan AT, Prasad BS, Anbazhagan S. Screening of in vitro anti-inflammatory activity of Ficus virens bark. J Glob Trends Pharm Sci 2014;5:2034-6.

Govindappa M, Sravya SN, Poojashri MN, Sadananda TS, Chandrappa CP. Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedelia trilobata (L.) Hitchc. J Pharmacog Phytother 2011;3:43-51.

Saumya D, Sanjita D, Kumar DM, Saumya PB. Evaluation of anti-inflammatory effect of Calotropis gigantea and Tridax procumbens on wistar albino rats. J Pharm Sci Res 2009;1:123-6.

Iqbal Z, Iqbal MS, Mishra K. Screening of antioxidant property in medicinal plants belonging to the family apocynaceae. Asian J Pharm Clin Res 2017;10:415-8.

Leelaprakash G, Mohan DS. In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.

Mandal SC, Maity TK, Das J, Saba BP, Pal M. Anti-inflammatory evaluation of Ficus racemosa Linn. Leaf extract. J Ethnopharmacol 2000;72:87-92.

Anoop MV, Bindu AR. In-vitro anti-inflammatory activity studies on Syzygium zeylanicum (L.) DC leaves. Int J Pharm Res Rev 2015;4:18- 27.

Leelaprakash G, Caroline RJ, Mohan DS. In vitro anti-inflammatory activity of Momordica charantia by inhibition of lipoxygenase enzyme. Int J Pharm Pharm Sci 2012;4:148-52.

Johora S, Kabir AS, Abdul H, Abdullah M, Islam N, Abu R. Evaluation of the antioxidant activity of Ficus racemosa plant extracts from north-western district of Bangladesh. J Life Earth Sci 2013;8:93-9.

Jahan IA, Nahar N, Mosihuzzaman M, Begum MR, Ali L, Khan AK, et al. Hypoglycaemic and antioxidant activities of Ficus racemosa Linn. fruits. Nat Prod Res 2009;23:399-408.

Hamid K, Sultana S, Urmi KF, Ullah MO, Zulfiker AH, Hossain MA. In vitro free radical scavenging and brine shrimp lithality bioassay of aqueous extract of Ficus racemosa seed. Jordan J Biol Sci 2011;4:51-4.

Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 2004;74:2157-84.

Dragland S, Senoo H, Wake K, Holte K, Blomhoff R. Several culinary and medicinal herbs are important sources of dietary antioxidants. J Nutr 2003;133:1286-90.

Akinmoladun AC, Obuotor EM, Farombi EO. Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. J Med Food 2010;13:444-51.

Özen T, Çöllü Z, Korkmaz H. Antioxidant properties of Urtica pilulifera root, seed, flower, and leaf extract. J Med Food 2010;13:1224-31.

Kaur N, Kishore K. Antioxidant activity of methanolic extract of Phaseolus trilobus root powder. Int J Pharm Pharm Sci 2012;4:271-5.

Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Smith PT, et al. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds. J Agric Food Chem 2011;59:12361-7.

Talhouk RS, Karam C, Fostok S, El-Jouni W, Barbour EK. Anti-inflammatory bioactivities in plant extracts. J Med Food 2007;10:1-0.

Wang C, Levis GB, Lee EB, Levis WR, Lee DW, Kim BS, et al. Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-[alpha] in activated RAW 264.7 cells. Int J Immunopharmacol 2004;4:1039-49.

Chohan M, Naughton DP, Jones L, Opara EI. An investigation of the relationship between the anti-inflammatory activity, polyphenolic content, and antioxidant activities of cooked and in vitro digested culinary herbs. Oxid Med Cell Longev 2012;2012:627843, 9.




About this article

Title

IN VITRO STUDY OF ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF SOME MEDICINAL PLANTS AND THEIR INTERRELATIONSHIP

Topics

Biotechnology

Keywords

Pharmacology

DOI

10.22159/ajpcr.2018.v11i4.23583

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 195-202

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Shubhi Rastogi
Amity Research Cell, Amity University Uttar Pradesh, Lucknow Campus, India
India

Mohammed Shariq Iqbal
Amity Research Cell, Amity University Uttar Pradesh, Lucknow Campus, India
India

Deepak Ohri
Amity Research Cell, Amity University Uttar Pradesh, Lucknow Campus, India
India


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