• Gana Manjusha Kondepudi Department of Pharmacognosy and Phytochemistry, Vignan Institute of Pharmaceutical Technology, Besides Vsez, Kapujaggraju Peta Duvvada, Visakhapatnam, Andhra Pradesh, India.




Anthelmintic, Mangroves, Pheretima posthuma, Vermifuge, Vermicide


 Objectives: The purpose of the present study was to determine the anthelmintic properties of chloroform, methanol and aqueous extracts of Avicennia marina leaves on Indian earthworm- Pheretima posthuma.

Methods: Three concentrations of each extract (25 mg/ml, 50 mg/ml, and 75 mg/ml) were studied in this activity to determine the time of paralysis (vermifuge) and the time of the death of the worms (vermicidal). Piperazine citrate in the same concentrations was taken a reference standard and normal water was taken as control. The time taken for paralysis and death of the worms was recorded.

Results: All the extracts showed dose-dependent and a significant activity was shown at higher concentration (75 mg/ml). Methanolic extract showed peak activity at a concentration of 75 mg/ml.

Conclusion: Based on the results it was found that the leaves of A. marina possess vermifuge and vermicidal activities and can be used as an effective anthelmintic.


Download data is not yet available.

Author Biography

Gana Manjusha Kondepudi, Department of Pharmacognosy and Phytochemistry, Vignan Institute of Pharmaceutical Technology, Besides Vsez, Kapujaggraju Peta Duvvada, Visakhapatnam, Andhra Pradesh, India.

department of pharmacognosy and phytochemistry

assistant professor


Atolani O, Olatunji GA. Comprehensive scientific demystification of Kigelia Africana: A review. Afr J Pure Appl Chem 2009;3:158-64.

Iswariya S, Uma TS. Evaluation of in vitro anti-inflammatory and antimicrobial activity of aqueous and methanolic seed extracts of Citrullus lanatus. Int J Pharm Pharm Sci 2017;9:29-3.

Bandaranayake WM. Traditional and medicinal uses of mangroves. Mangroves Salt Marshes 1998;2:133-48.

Duke NC. A systematic revision of mangroves genus Avicennia (Avicenniaceae) in Australia. Aust Syst Bot 1991;4:299-24.

Kosalge SB, Fursule RA. Investigation of in vitro Anthelmintic activity of Thespesia lampas (cav.). Asian J Pharm Clin Res 2009;2:69-71.

Bundy DA. Immunoepidemiology of intestinal helmintic infection I: The global burden of intestinal nematode disease. Trans Royal Soc Trop Med Hyg 1994;8:259-61.

Tagbota S, Townson S. Antiparasitic properties of medicinal and other naturally occurring products. Adv Parasitol 2001;50:199-5.

Olounlade’PA, Azando EV, Hounzangbe’-adote’ MS, Ha TB, Leroy E, Moulis C, et al. Inivitro anthelmintic activity anthelmintic activity of the essential oils of Zanthoxylum zanthoxyloides and Newbouldia laevis against Strongyloides ratti. Parasito Res 2012;110:1427-33.

Mehlhorn H, Al-Quraishy S, Al-Rasheid KA, Jatzlau A, Abdel-Ghaffar F. Addition of combination of onion (Allium cepa) and coconut(Cocos nucifera) to food of sheep stops gastrointestinal infections. Parasito Res 2010;108:1041-6.

Vigar Z. Atlas of Medical Physiology. 2nd ed. Singapore: P.G. Publishing House; 1984.

Dash GK, Suesh P, Kar DM, Ganapaty S, Panda SB. Evaluation of Evolvulus alsinoides Linn. For anthelmintic and antimicirobial activities. J Nat Rem 2002;2:182-5.

Jayaramu SC, Prathiba M. Evaluation of in vitro antihelmintic properties of the extract of Russelia equisetiformis (schlecht and cham) scrophulariaceae. Int J Pharm Pharm Sci 2016;8:459-61.

Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropis gynandra extract. Pharm Biol 2001;39:217-20.

Deb PK, Ghosh R, Das S, Bhakta T. In vitro anthelmintic activity of Acorus calamus leaves. Asian J Pharm Clin Res 2013;6:135-7.



How to Cite

Kondepudi, G. M. “EVALUATION OF IN VITRO ANTHELMINTIC PROPERTIES OF AVICENNIA MARINA LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 4, Apr. 2018, pp. 303-5, doi:10.22159/ajpcr.2018.v11i4.23605.



Original Article(s)