COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE


Neelu Sree P, Terin J, Himadri Dutta, Kalyani M

Abstract


 Objectives: The objectives of the study were to find out the presence of Mycobacterium tuberculosis in sputum samples using Ziehl-Neelsen (ZN), Kinyoun’s, and (auramine) fluorescent staining and to compare the three staining techniques with and without Petroff’s concentration and to find out the most preferable staining of M. tuberculosis.

Methods: Sputum sample was collected and concentrated by 4% NaOH (Petroff’s concentration). Microscopic examination of the sample was done before concentration and after concentration by ZN staining, Kinyoun’s staining, and fluorescent staining (Auramine). Grading of acid-fast bacilli (AFB) by three staining was done before and after concentration according to RNTCP guidelines.

Results: Total of 452 sputum samples were collected and subjected to microscopy examination by ZN, Kinyoun’s, and fluorescent staining methods to compare the presence or absence of AFB with or without concentration. Among the 452 sputum samples, total of 67 (15.6%) sputum samples were positive for the presence of AFB. Majority 40.3% (n=21) of TB positive patients were observed in the age group 51–60 years. The results of Auramine-O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100% and specificity 95.6%.

Conclusion: The use of fluorescent staining significantly increases the diagnostic value of the smear, particularly where there are low-density bacilli which may escape detection on ZN stained smears.


Keywords


Mycobacterium tuberculosis, Ziehl-Neelsen, Kinyoun’s staining, Fluorescent staining, Petroff’s concentration technique.

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References


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About this article

Title

COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE

Topics

Clinical Microbiology

Keywords

Mycobacterium tuberculosis, Ziehl-Neelsen, Kinyoun’s staining, Fluorescent staining, Petroff’s concentration technique.

DOI

10.22159/ajpcr.2018.v11i4.23662

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 110-113

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

24 Views | 68 Downloads

Authors & Affiliations

Neelu Sree P
Department of Microbiology, Saveetha Medical College, Thandalam, Chennai, Tamil Nadu, India
India

Terin J
Department of Microbiology, Saveetha Medical College, Thandalam, Chennai, Tamil Nadu, India
India

Himadri Dutta
Department of Microbiology, Saveetha Medical College, Thandalam, Chennai, Tamil Nadu, India

Kalyani M
Department of Microbiology, Saveetha Medical College, Thandalam, Chennai, Tamil Nadu, India
India


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