• Boudjada Amina Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.
  • Touil Ahmed Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.
  • Bendif Hamdi Department of Natural and Life Science, Faculty of Science, Mohamed Boudiaf University, BP 166 M’sila 28000, Algeria.
  • Bensouici Chawki Centre de Recherche en Biotechnologie, Ali Mendjli, UV 03, BP E73,Constantine, Algeria.
  • Rhouati Salah Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.


 Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.

Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.

Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.

Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.

Keywords: Crataegus azarolus (L.), Volatile compounds, Flavonol glycosides, Phenolic contents, Antioxidant activity.


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How to Cite
Amina, B., T. Ahmed, B. Hamdi, B. Chawki, and R. Salah. “PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 4, Apr. 2018, pp. 133-7, doi:10.22159/ajpcr.2018.v11i4.23724.
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