PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS


Boudjada Amina, Touil Ahmed, Bendif Hamdi, Bensouici Chawki, Rhouati Salah

Abstract


 Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.

Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.

Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.

Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.


Keywords


Crataegus azarolus (L.), Volatile compounds, Flavonol glycosides, Phenolic contents, Antioxidant activity.

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About this article

Title

PHYTOCHEMICAL CONSTITUENTS, PHENOLIC CONTENTS, AND ANTIOXIDANT ACTIVITY OF CRATAEGUS AZAROLUS EXTRACTS

Keywords

Crataegus azarolus (L.), Volatile compounds, Flavonol glycosides, Phenolic contents, Antioxidant activity.

DOI

10.22159/ajpcr.2018.v11i4.23724

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 133-137

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Boudjada Amina
Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.
Algeria

Touil Ahmed
Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.
Algeria

Bendif Hamdi
Department of Natural and Life Science, Faculty of Science, Mohamed Boudiaf University, BP 166 M’sila 28000, Algeria.
Algeria

Bensouici Chawki
Centre de Recherche en Biotechnologie, Ali Mendjli, UV 03, BP E73,Constantine, Algeria.
Algeria

Rhouati Salah
Laboratory of Natural Product from Plant and Organic Synthesis, Department of Chemistry, Faculty of Science, University of Mentouri‑Constantine, Route Ain el-Bey, 25000, Constantine, Algeria.
Algeria


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