SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC ACTIVITY OF BAUHINIA RACEMOSA LAM. LEAVES

Bhimraj Gawade; Mazahar Farooqui

doi:10.22159/ajpcr.2018.v11i6.24038

Authors

Bhimraj Gawade Department of Chemistry, A. D. College, Kada, Maharashtra, India.
Mazahar Farooqui Department of Chemistry, Dr. Rafiq Zakaria College for Women,Aurangabad, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.24038

Keywords:

Bauhinia racemosa Lam, Pharmacological, Nil, Phytochemical, Antidiabetic

Abstract

Objective: In this study, the leaves of medicinal plant Bauhinia racemosa Lam. with different pharmacological activities were subjected to phytochemical screening and assessment of their in vitro inhibitory potential with porcine pancreatic Î±-amylase enzyme to treat and management of diabetes.

Methods: Plant leaves were extracted sequentially with ethanol solvent. A modified 3,5-dinitrosalicylic acid method was adopted to screen Î±-amylase inhibition assay. The ethanol extract was analyzed qualitatively and gas chromatographyâ€“mass spectrometry analysis technique for the active phytoconstituents according to the standard protocols.

Results: A phytochemical screening of leaves extract reveals the presence of carbohydrate, alkaloids, saponin, glycosides, steroids, tannins, flavonoids, triterpenoid, and phenolic compounds. The ethanol extract reported inhibition of Î±-amylase enzyme activity at IC50 value 61.72 Â± 0.03 Î¼g/mL and acarbose as a standard drug at IC50 value 28.07 Â± 0.02 Î¼g/mL.

Conclusion: The results of the study indicate that B. racemosa Lam. leaves contain some of bioactive phytochemicals might to be exhibiting in vitro antidiabetic activity, which was leading to decreases the rate of starch digestion.

Downloads

Download data is not yet available.

References

Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.

Lamer J. The Pharmacological Basis of Therapeutics. 7th ed. New York: MacMillan; 1985.

WHO. World Health Organization Expert Committee on Diabetes Mellitus. Second Report, Technical Report Series. Geneva: WHO; 1980.

Shettar AK, Vedamurthy AB. Studies on in vitro antidiabetic activities of Hopea ponga and Vitex leucoxylon. Int J Pharm Pharm Sci 2017;9:263- 7.

Gupta M, Mazumder UK, Kumar RS, Gomathi P, Rajeshwar Y, Kakoti BB, et al. Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models. J Ethnopharmacol 2005;98:267-73.

El-Khatiba AS, Khaleel AE. Evaluation of some pharmaco-logical properties of different extract of Bauhinia racemosa leaf and Bassia muricata whole plant. Bull Fac Pharm Cairo Univ 1995;33:59-65.

Panda P, Das D, Dash P, Ghosh G. Therapeutic potential of Bauhinia racemosa-A mini review. Int J Pharm Sci Rev Res 2015;32:169-79.

Panda P, Pal A, Maharana L, Ghosh G. Antibacterial and antioxidant activities of methanolic extract of Bauhinia racemosa. Int J Pharm Pharm Sci 2016;8:277-81.

Kumar RS, Sivakumar T, Sunderam RS, Gupta M, Mazumdar UK, Gomathi P, et al. Antioxidant and antimicrobial activities of Bauhinia racemosa L. Stem bark. Braz J Med Biol Res 2005;38:1015-24.

Tyagi R, Shukla AA, Shukla RK. Phytochemical screening and pharmacological evaluation of different extracts of plant Casearia tomentosa leaves. Int J Pharm Pharm Sci 2017;9:166-71.

Sahu T, Sahu J. Buahinia racemosa (Kachnar): A Review of its medicinal properties. World J Pharm Res 2015;4:686-96.

Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. J Ethnopharmacol 2014;155:857-924.

Behera1 B, Yadav D. Current researches on plants having antidiabetic potential: An overview, research and reviews. J Bot Sci 2013;2:4-17.

Bhutkar MA, Bhise SB. In vitro assay of alpha amylase inhibitory activity of some indigenous plants. Int J Chem Sci 2012;10:457-62.

Gawade BN, Farooqui M. In vitro alpha amylase inhibitory activity study of Zizyphus mauritiana leaves ethanol extract. J Med Chem Drug Disc 2017;3:67-76.

Ghumare P, Jirekar DB, Farooqui M, Naikwade SD. Phytochemical analysis of petroleum ether extract of some selected medicinal plants leaves. Indian J Adv Plant Res 2014;1:20-3.

Tiwari P, Kumar B, Kaur M. Phytochemical screening and extraction: A review. Int Pharm Sci 2011;l:98-106.

Ghumare P, Jirekar DB, Farooqui M, Naikwade SD. Biological activity of aqueous extract of some medicinal plants. Der Chem Sin 2014;5:65-70.

Soni V, Jha AK, Dwivedi J, Soni P. Traditional uses, phytochemistry and pharmacology of Bauhinia racemosa Lam-a review. TANG 2015;5:1-7. Available from: http://www.e-tang.org.

Kulkarni SS, Lanjewar RB, Gadegone SM. A review on levodopa and beta-sitosterol and its pharmacological actions in Bauhinia racemosa, Canavalia gladiata, Vigna vexillata medicinal Plants. J Med Plants Stud 2016;4:259-64.

Pawar DS, Nasreen S. Phytochemical screening and antifungal activity of Bauhinia racemosa and Dolichandrone falcate. Int J Appl Res 2016;2:243-5.

Krishnamoorthi R. Phytochemical screening and antioxidant activity of Justicia tranquebariensis and Bauhinia racemosa. Int J Pharm 2015;2:362-7.

Ghumare P, Jirekar DB, Farooqui M, Naikwade SD. Preliminary phytochemical screening and antibacterial activity of Bauhinia racemosa Leaves. Biosci Biotech Res Asia 2014;11:1-4.

Akelesh T, Gopal V, Jeyabalan G, Johnson S, Chander JU, Manavalan R, et al. Preliminary phytochemical screening of the extracts of Bauhinia racemosa Linn. World J Pharm Pharm Sci 2017;6:1565-72.

Killari KN, Konduri P, Shaik A, Reddy Vennapusa JK, Talluri M. Protective effect of Bauhinia racemosa against streptozotocin induced diabetes male infertility complications. Int Res J Pharm 2017;8:74-9.

Kumar V, Rathore K, Jain P, Ahmed Z. Biological activity of Bauhinia racemosa against diabetes and interlinked disorders like obesity and hyperlipidemia. Clin Phytosci 2017;3:1-9.

Kanimozhi D, Bai VR. Analysis of bioactive components of ethanolic extract of Coriandrum sativum L. Int J Res Pharm Sci 2012;2:97-110.

Kalpana S, Ramakrushna B, Anitha S. Evaluation of in vitro antioxidant and Î±-amylase inhibitory activity of Phyllanthus indofischeri Bennet. Int J Pharm Pharm Sci 2016;8:131-6.