THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL


Emma Kamelia, Andi Asadul Islam, Mochammad Hatta, Cahyono Kaelan, Ilhamjaya Patellongi

Abstract


 Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)

Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.

Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).

Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.


Keywords


Alzheimer’s disease, Intracerebroventricularly, Amyloid beta, Caspase-3, Musa paradisiaca L.

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About this article

Title

THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL

Topics

medical science

Keywords

Alzheimer’s disease, Intracerebroventricularly, Amyloid beta, Caspase-3, Musa paradisiaca L.

DOI

10.22159/ajpcr.2018.v11i4.24103

Date

01-04-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Issue 4 April 2018 Page: 298-302

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Emma Kamelia
Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
Indonesia

Andi Asadul Islam
Department of Neurosurgery, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
Indonesia

Mochammad Hatta
Molecular Biology and Immunology Laboratory for Infection Diseases, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
Indonesia

Cahyono Kaelan
Department of Neurology, Medical Faculty, University of Hasanuddin Makassar, Indonesia.
Indonesia

Ilhamjaya Patellongi
Department of Physiology, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
Indonesia


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