THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL

  • Emma Kamelia Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia. http://orcid.org/0000-0002-7601-4746
  • Andi Asadul Islam Department of Neurosurgery, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Mochammad Hatta Molecular Biology and Immunology Laboratory for Infection Diseases, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Cahyono Kaelan Department of Neurology, Medical Faculty, University of Hasanuddin Makassar, Indonesia.
  • Ilhamjaya Patellongi Department of Physiology, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.

Abstract

 Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)

Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.

Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).

Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.

Keywords: Alzheimer’s disease, Intracerebroventricularly, Amyloid beta, Caspase-3, Musa paradisiaca L.

Author Biography

Emma Kamelia, Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
Dentistry, Medical science, microbiology

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Kamelia, E., A. Asadul Islam, M. Hatta, C. Kaelan, and I. Patellongi. “THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 4, Apr. 2018, pp. 298-02, doi:10.22159/ajpcr.2018.v11i4.24103.
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