• Emma Kamelia Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
  • Andi Asadul Islam Department of Neurosurgery, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Mochammad Hatta Molecular Biology and Immunology Laboratory for Infection Diseases, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Cahyono Kaelan Department of Neurology, Medical Faculty, University of Hasanuddin Makassar, Indonesia.
  • Ilhamjaya Patellongi Department of Physiology, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.


 Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)

Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.

Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).

Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.

Keywords: Alzheimer’s disease, Intracerebroventricularly, Amyloid beta, Caspase-3, Musa paradisiaca L.

Author Biography

Emma Kamelia, Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
Dentistry, Medical science, microbiology


1. Aggarwal NT, Shah RC, Bennett DA. Alzheimer’s disease: Unique markers for diagnosis and new treatment modalities. Indian J Med Res 2015;142:369-82.
2. Alzheimer’s association. Alzheimer’s disease facts and figures. Alzheimers Dement 2012;8:131-68.
3. Seshadri S, Wolf PA. The Lifetime risk of stroke and dementia: Current concepts, and estimates from the Framingham Study. Lancet Neurol 2007;6:1106-14.
4. Rubinsztein DC. The roles of intracellular protein-degradation pathways in neurodegeneration. Nature 2006;443:780-6.
5. Arif NS, Kasi, Devi P. Botanics: A potential source of new therapies for Alzheimer’s disease? Botanics 2014;4:11-26.
6. Earnshaw WC, Martins LM, Kaufmann SH. Mammalian caspases: Structure, activation, substrates, and functions during apoptosis. Ann Rev Biochem 1999;68:383-424.
7. Ashkenazi A, Dixit VM. Death receptors: Signaling and modulation. Science 1998;281:1305-8.
8. Wolf BB, Schuler M, Echeverri F, Green DR. Caspase-3 is the primary activator of apoptotic DNA fragmentation via DNA fragmentation factor-45/inhibitor of caspase-activated DNase inactivation. J Biol Chem 1999;274:30651-6.
9. Kuida K, Zheng TS, Na S, Kuan C, Yang D, Karasuyama H, et al. Deceased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 1996;384:368-72.
10. Khan SM, Cassarino DS, Abramova NN, Keeney PM, Borland MK, Trimmer PA, et al. Alzheimer’s disease cybrids replicate b-amyloid abnormalities through cell death pathways. Ann Neurol 2000;48:148 55.
11. Commenges D, Scotet V, Renaud S, Jacqmin-Gadda H, Barberger-Gateau P, Dartigues JF. Intake of flavonoids and risk of dementia. Eur J Epidemiol 2000;16:357-63.
12. Ittiyavirah S, Anurenj DA. Adaptogenic studies of acetone extract of Musa paradisiaca L. fruit peels in albino Wistar rats. Int J Nutr Pharm Neurol Dis 2014;4:88-94.
13. Kamelia E, Miko H, Karo M, Pertiwi S, Kaelan C, Islam AA, et al. Beta amyloid peptide role in animal modeling trial of Alzheimer’s disease. Int J Sci Basic Appl Res 2016;28:90-9.
14. Hatta M, Smits HL. Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples. Am J Trop Med Hyg 2007;76:139-43.
15. Cai Y, Li J, Yang S, Li P, Zhang X, Liu H. CIBZ, a Novel BTB Domain-containing protein, is involved in mouse spinal cord injury via mitochondrial pathway independent of p53 gene. PLoS One 2012;7:e33156.
16. Cecilia KZ, Mona-Lisa S, Söder O. The high mobility group box chromosomal protein 1 is expressed in the human and rat testis where it may function as an antibacterial factor. Hum Reprod 2006;21:2801-9.
17. Cetin F, Yazihan N, Dincer S, Akbulut KG. The effect of intracerebroventricular injection of beta amyloid peptide (1-42) on caspase 3 activity, lipid peroxidation, nitrit oxide and NOS expression in young adult and aged rat brain. Turk Neurosurg 2013;23:144-50.
18. Vauzour D. Dietary polyphenols as modulators of brain functions: Biological actions and molecular mechanisms underpinning their beneficial effects. Oxid Med Cell Longev 2012;2012:914273.
19. Nunomura A, Moreira PI, Castellani RJ, Lee HG, Zhu X, Smith MA, Perry G. Oxidative damage to RNA in aging and neurodegenerative disorders. Neurotox Res 2012;22:231.
20. Kiranjit K, Rajneet K, Manjinder K. Review Article: Recent advances in Alzheimer’s disease: Cause and treatment. Int J Pharm Pharm Sci 2016;8:8-15.
21. Hengartner MO. The biochemistry of apoptosis. Nature 2000;407:770- 6.
22. Vijayakumar S, Presannakumar G, Vijayalakshmi N. Antioxidant activity of banana flavonoids. Fitoterapia 2008;79:279-82.
23. Hwang SL, Yen GC. Modulation of Akt, JNK, and p38 activation is involved in citrus flavonoid-mediated cytoprotection of PC12 cells challenged by hydrogen peroxide. J Agric Food Chem 2009;57:2576- 82.
24. Friedlander R. Apoptosis and caspases in neurodegenerative diseases. N Engl J Med 2003;348:1365-75.
25. Bashir AA, Khamsah SM, Muhammad A, Mahadeva Rao US, Than Z. Phitochemical screening, antioxidant activity of pure syringing in comparison to various solvent extracts of Musa paradisiacal (Banana) (fruit and flower) and total phenolic content. Int J Pharm Pharm Sci 2015;7:242-7.
153 Views | 272 Downloads
How to Cite
Kamelia, E., A. Asadul Islam, M. Hatta, C. Kaelan, and I. Patellongi. “THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 4, Apr. 2018, pp. 298-02, doi:10.22159/ajpcr.2018.v11i4.24103.
Original Article(s)