• Emma Kamelia Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
  • Andi Asadul Islam Department of Neurosurgery, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Mochammad Hatta Molecular Biology and Immunology Laboratory for Infection Diseases, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.
  • Cahyono Kaelan Department of Neurology, Medical Faculty, University of Hasanuddin Makassar, Indonesia.
  • Ilhamjaya Patellongi Department of Physiology, Medical Faculty, University of Hasanuddin, Makassar, Indonesia.


 Objective: The objective of this study is to analyze caspase-3 mRNA gene expression in a Wistar rat model of Alzheimer’s disease (AD) treated with Musa paradisiaca L. (MPL) ethanol extract or banana extract (BE)

Methods: MPL was evaluated for its effect on the caspase-3 mRNA gene expression of rat amyloid beta (Aβ) induced, an AD model. Each model included twenty Wistar rats were randomized into five groups: K0, without AD induction and no BE; K1, AD induction and no BE; K2, AD induction+BE 250 mg/kg body weight (BW); K3, AD induction+BE 500 mg/kg; and K4, AD induction+BE 1000 mg/kg. AD induction was performed by Aβ1-42 (0.2 ug) injection at the intracerebroventricular area. mRNA caspase-3 level measurements were performed by real time-polymerase chain reaction.

Results: Paired t-test analysis showed no significant differences of caspase-3 mRNA level before Aβ induction among five groups (p>0.05). At 6 weeks post-Aβ induction, there was significantly increased caspase-3 mRNA in all groups except K0 (p<0.05). Notably, after 3 weeks of BE administration, caspase-3 mRNA expression was significantly decreased in all BE-treated groups; in the K1 placebo group, caspase-3 mRNA expression increased. The maximum decreased caspase-3 mRNA expression was in group K4 (-BE 1000 mg/kg BW), and the minimum was in group K2 (-BE 250 mg/kg BW).

Conclusion: The results revealed that the ethanolic extract of MPL fruit could decrease caspase-3 mRNA gene expression in AD rat.

Keywords: Alzheimer’s disease, Intracerebroventricularly, Amyloid beta, Caspase-3, Musa paradisiaca L.

Author Biography

Emma Kamelia, Dental Health Study Program, Tasikmalaya Health Polytechnic, West Java, Indonesia.
Dentistry, Medical science, microbiology


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How to Cite
Kamelia, E., A. Asadul Islam, M. Hatta, C. Kaelan, and I. Patellongi. “THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT FROM MUSA PARADISIACA L. (MPL) FRUIT ON THE CASPASE-3 MRNA GENE EXPRESSION IN RAT AMYLOID BETA INDUCED, AN ALZHEIMER’S DISEASE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 4, Apr. 2018, pp. 298-02, doi:10.22159/ajpcr.2018.v11i4.24103.
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