A MYCOLOGICAL STUDY OF CLINICAL SAMPLES FROM SUSPECTED MYCOSES IN A TERTIARY CARE HOSPITAL

Jitendra Chandra Devrari; Varsha Saxena; Vidya Pai

doi:10.22159/ajpcr.2018.v11i4.24127

Authors

Jitendra Chandra Devrari Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India
Varsha Saxena Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India
Vidya Pai Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i4.24127

Keywords:

Mycoses, Dermatophytes, Candida species, Aspergillus fumigatus, Fusarium species

Abstract

Â Objective: The objective of this study was to isolate and identify the fungal agents obtained from clinical samples of suspected mycoses patients attending Yenepoya hospital.

Methods: Various clinical samples obtained from patients were subjected to preliminary examination according to standard mycological protocols such as KOH mount, wet mount, Gram's staining, and Indian ink examination. Causative organisms were identified by macroscopically and microscopically after growth on sabouraud's dextrose agar (with or without cycloheximide and chloramphenicol) and confirmed by lactophenol cotton blue mount, slide culture technique, urease test, and growth on chrome agar accordingly.

Results: Of 274 clinical samples, 125 were culture positive in which the fungal isolates obtained were dermatophytes (all 3 genera), Candida species (including Candida albicans), Fusarium species, Aspergillus fumigatus, and Cryptococcus neoformans. Male predominance was found among the patients being 6:3 male:female ratio.

Conclusion: Among the superficial infection, Trichophyton mentagrophytes was found to be predominant isolates; however, in systemic infection, C. albicans was the predominant isolates.

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References

Baron S, editor. Medical Microbiology. 4th ed. Galveston (TX): University of Texas Medical Branch at Galveston; 1996.

Lakshmanan A, Ganeshkumar P, Mohan SR, Hemamalini M, Madhavan R. Epidemiological and clinical pattern of dermatomycoses in rural India. Indian J Med Microbiol 2015;33:134-6.

Tonita M, Noronha TM, Tophakhane RS, Nadiger S. Clinico microbiological study of dermatophytosis in a tertiary care hospital in North Karnataka. Indian Dermatol Online J 2016;7:264-71.

Ray A, Anand S. Recent trends in antifungal therapy: Focus on systemic mycoses. Indian J Chest Disease Allied Sci 2000;42:357-66.

Diba K, Kordbacheh P, Mirhendi SH, Rezaie S, Mahmoudi M. Identification of Aspergillus species using morphological characteristics. Pak J Med Sci 2007;23:867-72.

De Rosa FG, Garazzino S, Pasero D, Di Perri G, Ranieri VM. Invasive candidiasis and candidemia: New guidelines. Minerva Anestesiol 2009;75:453-8.

Kak S, Bhat RM, Boloor R, Nandakishore B, Sukumar DA. Clinical and mycological study of dermatophytic infections. Indian J Dermatol 2014;59:262-7.

Pai VV, Hanumanthayya K, Kikkeri NC. Clinical study of tinea capitis in Northern Karnataka: A three-year experience at a single institute. Indian Dermatol Online J 2013;4:22-6.

Malik A, Fatima N, Khan PA. A clinico-mycological study of superficial mycoses from a tertiary care hospital of a North Indian town. Virol Mycol 2014;3:135-8.

Kannan P, Janaki C, Selvi GS. Prevalence of dermatophytes and other fungal agents isolated from clinical samples. Indian J Med Microbiol 2006;24:212-5.

Kuruvilla TS, Dias M. Fusarium solani: A causative agent of skin and nail infections. Indian J Dermatol 2012;57:308-9.

Jandial S, Sumbali G. Fusarial onychomycosis among gardeners: A report of two cases. Indian J Dermatol Venereol Leprol 2012;78:229- 30.

Khan S, Kumar A, Vinod B, Prabhakar V, Eapen M, Thomas J, et al. Chromoblastomycosis due to Fonsecaea pedrosoi: An old wine in a rare bottle. J Infect Dev Ctries 2015;9:325-9.