A MYCOLOGICAL STUDY OF CLINICAL SAMPLES FROM SUSPECTED MYCOSES IN A TERTIARY CARE HOSPITAL
Â Objective: The objective of this study was to isolate and identify the fungal agents obtained from clinical samples of suspected mycoses patients attending Yenepoya hospital.
Methods: Various clinical samples obtained from patients were subjected to preliminary examination according to standard mycological protocols such as KOH mount, wet mount, Gramâ€™s staining, and Indian ink examination. Causative organisms were identified by macroscopically and microscopically after growth on sabouraudâ€™s dextrose agar (with or without cycloheximide and chloramphenicol) and confirmed by lactophenol cotton blue mount, slide culture technique, urease test, and growth on chrome agar accordingly.
Results: Of 274 clinical samples, 125 were culture positive in which the fungal isolates obtained were dermatophytes (all 3 genera), Candida species (including Candida albicans), Fusarium species, Aspergillus fumigatus, and Cryptococcus neoformans. Male predominance was found among the patients being 6:3 male:female ratio.
Conclusion: Among the superficial infection, Trichophyton mentagrophytes was found to be predominant isolates; however, in systemic infection, C. albicans was the predominant isolates.
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