• Mohammad Rizki Fadhil Pratama Department of Pharmacy, Faculty of Health Sciences, Muhammadiyah University of Palangkaraya, Palangka Raya, Central Borneo, Indonesia.
  • Sutomo S Pharmacy Study Programme, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat, Banjarmasin, South Borneo, Indonesia.


Objectives: Lupeol, a triterpenoid isolated from Kasturi (Mangifera casturi) fruit has been known for having several pharmacological activities, including anticancer properties. Lupeol showed antiproliferative activity toward many cancer cells line including breast cancer. Lupeol showed promising potency as both ER-α and HER2 inhibitors, although still lower than known ER-α and HER2 Inhibitors. Chemical structure optimization of lupeol was predicted could increase the affinity of lupeol derivatives against ER-α and HER2. This study aims to determine lupeol derivative with the highest affinity against ER-α and HER2.

Methods: All ligands were sketched and optimized using Gaussian 03W with Hartree–Fock method basis set 3-21G. Molecular docking was performed using Autodock 4.2.6 on several modified chemical structure of lupeol against active site of ER-α and HER2. The main parameter used was the free energy of binding and inhibition constants as affinity marker.

Results: The docking results show that lupeol derivative with an amine group (Lupeol-2) and ethyl group (Lupeol-4) at position C3 provide the highest affinity with the free energy of binding and dissociation constant −12.24 kcal/mol and 1.07 nM for ER-α also −9.63 kcal/mol and 86.94 nM for HER2, respectively. Interestingly, although lupeol derivatives showed higher affinity toward ER-α, their amino acid residues were closer to the interaction on HER2.

Conclusion: These results predict that lupeol have greater potential to be developed as a HER2 inhibitor. Further, derivate lupeol-4 should be potential to be developed as HER2-positive breast cancer therapy.

Keywords: Breast cancer, Docking, ER-α, HER2, Lupeol.

Author Biographies

Mohammad Rizki Fadhil Pratama, Department of Pharmacy, Faculty of Health Sciences, Muhammadiyah University of Palangkaraya, Palangka Raya, Central Borneo, Indonesia.

Researcher from Pharmacy Department

Faculty of Health Sciences

Muhammadiyah University of Palangkaraya

RTA Milono st Km 1.5

Palangka Raya, Indonesia 73111

Sutomo S, Pharmacy Study Programme, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat, Banjarmasin, South Borneo, Indonesia.

Lecturer in Pharmacy Study Programme

Faculty of Mathematics and Natural Sciences

Universitas Lambung Mangkurat

Ahmad Yani st Km 36

Banjarbaru, Indonesia 70714


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How to Cite
Pratama, M. R. F., and S. S. “CHEMICAL STRUCTURE OPTIMIZATION OF LUPEOL AS ER-Α AND HER2 INHIBITOR”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 6, June 2018, pp. 298-03, doi:10.22159/ajpcr.2018.v11i6.24226.
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