• Rambhadur P Subedi Department of Biological Sciences, Ramniranjan Jhunjhunwala College, Ghatkopar (W), Mumbai - 400 086, Maharashtra,India
  • Rekha R Vartak Biology Olympiad Cell, Homi Bhabha Centre for Science Education-TIFR, Mankhurd, Mumbai - 400 088, Maharashtra, India.
  • Purushottam G Kale Department of Biological Sciences, Ramniranjan Jhunjhunwala College, Ghatkopar (W), Mumbai - 400 086, Maharashtra,



Lifespan, Ayurvedic, Bhasma, Oxidative stress


 Objective: Management of aging is one of the most actively researched areas in biology. Ayurvedic preparations such as Abhrak Bhasma (AB) have been used for improving longevity and regenerative therapy as well as in treating various ailments. Modes of action of such Ayurvedic preparations are poorly understood at molecular level. Current investigation tests the efficacy of AB in modulating various parameters linked to oxidative stress.

Methods: Drosophila melanogaster was used as a model system to test the efficacy of AB in inducing antioxidant machinery. Drosophila flies were fed on diet supplemented with AB at larval and adult stages. Subsequently, various parameters, catalytic as well as non-catalytic, related to antioxidant machinery were analyzed.

Results: AB has been found to modulate the activity of superoxide dismutase and catalase enzymes as well as the total reduced glutathione (GSH) content. Larvae and adults feeding on diet supplemented with AB exhibit significantly lower levels of total GSH content (decrease of about 40–70% for larvae, while 31–36% for adults) and without any conclusive effect on GSH:oxidized glutathione ratio, free radical scavenging capacity, and extent of lipid peroxidation. These larvae and adults fed on diet supplemented with AB also exhibited an increase in the level of transcription of cap n†collar C, heat shock protein 70, and catalase genes (≥1.5 fold, except in few cases).

Conclusion: Overall, AB alters various parameters linked to antioxidant machinery in D. melanogaster. The induced components may provide protection to the organism during stressful conditions.


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Author Biographies

Rambhadur P Subedi, Department of Biological Sciences, Ramniranjan Jhunjhunwala College, Ghatkopar (W), Mumbai - 400 086, Maharashtra,India

Department of Biological sciences

Rekha R Vartak, Biology Olympiad Cell, Homi Bhabha Centre for Science Education-TIFR, Mankhurd, Mumbai - 400 088, Maharashtra, India.

Biology Olympiad Cell

Purushottam G Kale, Department of Biological Sciences, Ramniranjan Jhunjhunwala College, Ghatkopar (W), Mumbai - 400 086, Maharashtra,

Department of Biological Sciences


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How to Cite

Subedi, R. P., R. R. Vartak, and P. G. Kale. “MODULATION OF OXIDATIVE STRESS BY ABHRAK BHASMA IN DROSOPHILA MELANOGASTER”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 5, May 2018, pp. 247-53, doi:10.22159/ajpcr.2018.v11i5.24472.



Original Article(s)