DETECTION OF ANTIBODIES TO HIV IN UNSTIMULATED WHOLE SALIVA WITH IMMUNOCHROMATOGRAPHIC TEST KIT USED FOR SERUM.

  • Chandrasekar Lakshmi Krithika SRM University
  • Sathasivasubramanian Sankarapandiyan
  • Kumarasamy Nagalingeswaran
  • Nalini Ganesan

Abstract

Objectives: To determine the sensitivity and specificity for human immunodeficiency virus (HIV) antibodies in saliva using an immunochromatographic
test kit used for serum for detection of antibodies specific to HIV-1/HIV-2 without any procedural optimization.
Methods: A study was carried out in 40 HIV patients, and 40 HIV free volunteers. 2 ml of unstimulated saliva was collected, using drooling method
into sterile pre-weighed plastic containers for 10 minutes. The samples were labeled with numbers and laboratory personnel were completely blind
to the specimens and analyzed with a kit used to determine antibodies in serum/blood. Analysis was performed using immunochromatographic test
kit-SD BIOLINE HIV-1/2 - rapid test procedure (WHO approved) - third generation, for the qualitative determination of antibodies of all isotypes
specific to HIV-1 and HIV-2. No procedural optimization was done. Appropriate statistical analysis was performed.
Results: Out of the 80 salivary samples, 38 were true positive, 40 were true negative and 2 were false negative for HIV. Sensitivity was 95%, specificity
was 100%, positive predictive value of 100%, negative predictive value of 95% and disease prevalence of 50% were obtained.
Conclusion: Saliva samples collected without any specialized devices, and any test modification can be used instead of serum in rapid test assays for
screening of HIV 1 and 2 antibodies and serve as a diagnostic tool.

Keywords: Saliva, Human immunodeficiency virus infection, Human immunodeficiency virus 1 and 2 antibodies, Rapid test.

Author Biography

Chandrasekar Lakshmi Krithika, SRM University

Lecturer,
Department of Oral Medicine and Radiology,
SRM Dental College,
Bharathi Salai, Ramapuram

Chennai

Tamil Nadu,

India.

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How to Cite
Krithika, C. L., S. Sankarapandiyan, K. Nagalingeswaran, and N. Ganesan. “DETECTION OF ANTIBODIES TO HIV IN UNSTIMULATED WHOLE SALIVA WITH IMMUNOCHROMATOGRAPHIC TEST KIT USED FOR SERUM.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 1, Jan. 2015, pp. 164-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/2577.
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