• Gowrisankar L Department of Chemistry, Karpagam College of Engineering, Coimbatore - 641 032, Tamil Nadu, India, India.
  • Ganesh Murali J Department of Mechanical Engineering, Karpagam College of Engineering, Coimbatore - 641 032, Tamil Nadu, India.
  • Usha P Department of Science and Humanities, Faculty of Engineering, Karpagam Academy of Higher Education, Coimbatore, India.


The development of new materials and the enhancement of existing materials to develop skin regeneration are wide areas of research in polymeric biomaterials. The paper presents the analysis of a wide range of several natural polymers such as proteins and polysaccharides which can be utilized for skin tissue repair and regeneration. The reviews look at the few examples of commercially available natural - origin polymers with applications in tissue engineering. Natural polymers, such as proteins and polysaccharides, being components of, or structurally similar to, the glycosaminoglycans in the extracellular matrix (ECM) are valuable materials for tissue engineering applications. Natural polymers have great coincidence to natural ECM elements, particularly in biocompatibility and biodegradability. In this paper, the attention is focused on several natural polymers that found application in research work for drug or cell delivery within the skin tissue engineering field, namely collagen, chitin, chitosan, alginate, gellan, gelatin, and curcumin.

Keywords: Skin tissue engineering, Extracellular matrix, Regeneration, Biomolecules, Drug delivery.


1. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Lim CT, et al. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomater 2007;3:321-30.
2. Bottcher-Haberzeth S, Biedermann T, Reichmann E. Tissue engineering of skin. Burn 2010;36:450-60.
3. Murugan R, Huang ZM, Yang F, Ramakrishna S. Nanofibrous scaffold engineering using electrospinning. Nanosci Nanotechnol 2007;7:4595-3.
4. Kumbar SG, Nukavarapu SP, James R, Nair LS, Laurencin CT. Electrospun poly (lactic acid-co-glycolic acid) scaffolds for skin tissue engineering. Biomaterials 2008;29:4100-7.
5. Gupta SK, Dinda AK, Potdar PD, Mishra NC. Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications. Mater Sci Eng C 2013;33:4032-8.
6. Miyaji H, Sugaya T, Ibe K, Ishizuka R, Tokunaga K, Kawanami M. Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs. J Periodont Res 2010;45:58.
7. Shimoji S, Miyaji H, Sugaya T, Tsuji H, Hongo T, Nakatsuka M, et al. Bone perforation and placement of collagen sponge facilitate bone augmentation. J Periodont 2009;80:5.
8. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000;21:2529.
9. McKeon-Fischer KD, Flagg DH, Freeman JW. Coaxial electrospun poly(e-caprolactone), multiwalled carbon nanotubes, and polyacrylic acid/polyvinyl alcohol scaffold for skeletal muscle tissue engineering. J Biomed Mater Res A 2011;99:493.
10. Ma L, Gao C, Mao Z, Zhou J, Shen J, Hu X, et al. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials 2003;24:4833.
11. Chen F, Jin Y. Periodontal tissue engineering and regeneration: Current approaches and expanding opportunities. Tissue Eng Part B 2010;16:219.
12. Liao JF, Qu Y, Chu BY, Zhang XN, Qian ZY. Biodegradable CSMA/ PECA/grapheme porous hybrid scaffold for cartilage tissue engineering. Sci Rep UK 2015;5:9879.
13. Chen GP, Ushida T, Tateishi T. A biodegradable hybrid sponge nested with collagen microsponges. J Biomed Mater Res 2000;51:273-9.
14. Lahiji A, Sohrabi A, Hungerford DS, Frondoza CG. Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes. J Biomed Mater Res 2000;51:586-95.
15. Jaikumar D, Sajesh KM, Soumya S, Nimal TR, Chennazhi KP, Nair SV, et al. Injectable alginate-O-carboxymethyl chitosan/nano fibrin composite hydrogels for adipose tissue engineering. Int J Biol Macromol 2015;74:318-26.
16. Unnithan AR, Sasikala AR, Murugesan P, Gurusamy M, Wu DM, Park CH, et al. Electrospun polyurethane-dextran nanofiber mats loaded with estradiol for post-menopausal wound dressing. Int J Biol Macromol 2015;77:1-8.
17. Kavya KC, Jayakumar R, Nair S, Chennazhi KP. Fabrication and characterization of chitosan/gelatin/nSiO2composite scaffold for bone tissue engineering. Int J Biol Macromol 2013;59:255-63.
18. Burg KJL, Porter S, Kellam JF. Biomaterial developments for bone tissue engineering. Biomaterials 2000;21:2347-59.
19. Kim J, Kim IS, Cho TH, Lee KB, Hwang SJ, Tae G, et al. Bone regeneration using hyaluronic acid-based hydrogel with bone morphogenic protein-2 and human mesenchymal stem cells. Biomaterials 2007;28:1830-37.
20. Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL. Nanofiber technology: Designing the next generation of tissue engineering scaffolds. Adv Drug Delivery Rev 2007;59:1413-33.
21. Hutmacher DW, Goh JC, Teoh SH. An introduction to biodegradable materials for tissue engineering applications. Ann Acad Med Singapore 2001;30:183-91.
22. Park SN, Park JC, Kim HO, Song MJ, Suh H. Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide cross-linking. Biomaterials 2002;23:1205-12.
23. Eyre DR. Collagen: Molecular diversity in the body’s protein scaffold. Science 1980;207:1315-22.
24. Kemp PD. Tissue engineering and cell-populated collagen matrices. Methods Mol Biol 2000;139:287-93.
25. Lee CH, Singla A, Lee Y. Biomedical applications of collagen. Int J Pharmacogn 2001;221:1-22.
26. Desimone MF, Helary C, Giraud-Guille MM, Livage J, Coradin T. Fibroblast encapsulation in hybrid silica-collagen hydrogels. Mater J Chem 2009;20:666-8.
27. Xia Z, Yu X, Jiang X, Brody HD, Rowe DW, Wei M. Fabrication and characterization of biomimetic collagen-apatite scaffolds with tunable structures for bone tissue engineering. Acta Biomater 2013;9:7308-19.
28. Balakrishnan B, Mohanty M, Umashankar PR, Jayakrishnan A. Evaluation of an in situ forming hydrogel wound dressing based on oxidized alginate and gelatin. Biomaterials 2005;26:6335-42.
29. Ogawa M, Moody MW, Portier RJ, Bell J, Schexnayder MA, Losso JN. Biochemical properties of black drum and sheepshead sea bream skin collagen. J Agric Food Chem 2003;51:8088-92.
30. Jongjareonrak A, Benjakul S, Visessanguan W, Nagai T, Tanaka M. Isolation and characterisation of acid and pepsin-solubilised collagens from the skin of brownstripe red snapper. Food Chem 2005;93:475-84.
31. Wong Po Foo C, Kaplan DL. Genetic engineering of fibrous proteins: Spider dragline silk and collagen. Adv Drug Deliv Rev 2002;54:1131-43.
32. Anisha BS, Deepthi S, Annapoorna M, Chennazhi KP, Nair SV, Jayakumar R. Chitosan-hyaluronic acid/nano chondroitin sulphateternary composite sponges for biomedical use. Carbohydr Polym 2013;92:1470-6.
33. Yang TL. Chitin-based materials in tissue engineering: Applications in soft tissue and epithelial organ. Int J Mol Sci 2011;12:1936-63.
34. Anitha A, Sowmya S, Sudheeshkumar PT, Deepthi S, Chennazhi KP, Ehrlich H, et al. Chitin and chitosan in selected biomedical applications. Prog Polym Sci 2014;39:1644-67.
35. Dai T, Tanaka M, Huang, YY, Hamblin MR. Chitosan preparations for wounds and burns: Antimicrobial and wound-healing effects. Expert Rev Anti Infect Ther 2011;9:857-79.
36. Shalumon KT, Binulal NS, Selvamurugan N, Nair SV, Menon D, Furuike T, et al. Electrospinning of carboxymethyl chitin/poly (vinyl alcohol) nanofibrous scaffolds for tissue engineering applications. Carbohydr Polym 2009;77:863-9.
37. Granville-Chapman J, Jacobs N, Midwinter MJ. Pre-hospital hemostatic dressings: A systematic review. Injury 2011;42:447-59.
38. Jayakumar R, Prabaharan M, Sudheesh Kumar PT, Nair SV, Tamura H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol Adv 2011;29:322-37.
39. Nettles DL, Elder SH, Gilbert JA. Potential use of chitosan as a cell scaffold material for cartilage tissue engineering. Tissue Eng 2002;8:1009-16.
40. De Azevedo EP. Chitosan hydrogels for drug delivery and tissue engineering applications. Int J Pharm Pharm Sci 2015;7:8-14.
41. Nikpour MR, Rabiee SM, Jahanshahi M. Synthesis and characterization of hydroxyapatite/chitosan nanocomposite materials for medical engineering applications. Compos Part B Eng 2012;43:1881-6.
42. Mohandas A, Anisha BS, Chennazhi KP, Jayakumar R. Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids Surf B 2015;127:105-13.
43. Indrani DJ, Budiyanto E, Hayun H. Preparation and characterization of porous hydroxyapatite and alginate composite scaffolds for bone tissue engineering. Int J Applied Pharm 2017;9:98-2.
44. Cheung HY, Lau KT, Lu TP, Hui D. A critical review on polymer-based bioengineered materials for scaffold development. Compos Part B Eng 2007;38:291.
45. Bouhadir KH, Lee KY, Alsberg E, Damm KL, Anderson KW, Mooney DJ. Degradation of partially oxidized alginate and its potential application for tissue engineering. Biotechnol Prog 2001;17:945-50.
46. Teo SY, Lee SY, Rathbone MJ, Gan SN. Polymeric materials as platforms for topical drug delivery: A review. Int J Pharm Pharm Sci 2017;9:14-20.
47. Rowley JA, Madlambayan G, Mooney DJ. Alginate hydrogels as synthetic extracellular matrix materials. Biomaterials 1999;20:45-53.
48. Li X, Chen S, Zhang B, Li M, Diao K, Zhang Z, et al. In situ injectable nano-composite hydrogel composed of curcumin, N,O-carboxymethyl chitosan and oxidized alginate for wound healing application. Int J Pharm 2012;437:110-9.
49. Hooper SJ, Percival SL, Hill KE, Thomas DW, Hayes AJ, Williams DW. The visualisation and speed of kill of wound isolates on a silver alginate dressing. Int Wound J 2012;9:633-42.
50. Jansson PE, Lindberg B, Sandford PA. Structural studies of gellan gum, an extracellular polysaccharide elaborated by Pseudomonas elodea. Carbohydr Res 1983;124:35.
51. Moorhouse R, Colegrove GT, Sandford P, Baird K, Kang KS. A New Gel-forming Polysaccharide. Washington, DC: D.A. Brandt; 1981.
52. Rozier A, Mazuel C, Grove J, Plazonnet B. A novel, ion-activated, in-situ gelling polymer for ophthalmic vehicles. Effect on bioavailability of timolol. Int J Pharm 1989;57:163.
53. Grasdalen H, Smidsrod O. Gelation of gellan gum. Carbohydr Polym 1987;7:371.
54. Oliveira JT, Martins L, Picciochi R, Malafaya PB, Sousa RA, Neves NM, et al. Gellan gum: A new biomaterial for cartilage tissue engineering applications. J Biomed Mater Res A 2009;5:852-63.
55. Coviello T, Dentini M, Rambone G, Desideri P, Carafa M, Murtas E, et al. A novel cocrosslinked polysaccharide: Studies for a controlled delivery matrix. J Control Release 1998;55:57.
56. Wang H, Leeuwenburgh SC, Li Y. The use of micro-and nanospheres as functional components for tissue regerneration. Tissue Eng 2012;18:24-39.
57. Young S, Wong M, Tabata Y, Mikos AG. Gelatin as a delivery vehicle for the controlled release of bioactive molecules. J Control Release 2005;109:256-74.
58. Malda J, Kreijveld E, Temenoff JS, Blitterswijk CA, Riesle J. Expansion of human nasal chondrocytes on macroporous microcarriers enhances redifferentiation. Biomaterials 2003;24:153-61.
59. Payne RG, McGonigle JS, Yaszemski MJ, Yasko AW, Mikos AG. Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly (propylenefumarate). Biomaterials 2002;23:4373-80.
60. Kimura Y, Ozeki M, Inamoto T, Tabata Y. Adipose tissue engineering based on human preadip ocytes combined with gelatin microspheres containing basic fibroblast growth factor. Biomaterials 2003;24:2513-21.
61. Ponticiello MS, Schinagl RM, Kadiyala S, Barry FB. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J Biomed Mater Res 2000;52:246-55.
62. Sheth P, Myrdal PB. Polymers for pulmonary drug delivery. Controll Pulm Drug Deliv 2011;265-82.
63. Ammon HP, Wahl MA. Pharmacology of Curcuma longa. Planta Med 1991;57:1-7.
64. Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN, Kuttan R. Antitumour and antioxidant activity of natural curcuminoids. Cancer Lett 1995;94:79-83.
65. Vasanthkumar T, Manjunatha H, Rajesh KP. Anti-inflammatory activity of curcumin and capsaicin augmented in combination. Int J Pharm Pharm Sci 2017;9:145-9.
66. Vajragupta O, Boonchoong P, Morris GM, Olson AJ. Active site binding modes of curcumin in HIV-1 protease and integrase. Bioorg Med Chem Lett 200;15:3364-8.
67. Panchatcharam M, Miriyala S, Gayathri VS, Suguna L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem 2006;290:87-96.
68. Gopinath D, Ahmed MR, Gomathi K, Chitra K, Sehgal PK, Jayakumar R. Dermal wound healing processes with curcumin incorporated collagen films. Biomaterials 2004;25:1911-7.
69. Mitra T, Manna PJ, Raja ST, Gnanamanib AK, Undu PP. Curcumin loaded nano graphene oxide reinforced fish scale collagen–a 3D scaffold biomaterial for wound healing applications. RSC Adv 2015;5:98653-65.
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How to Cite
L, G., G. M. J, and U. P. “NATURAL BIOMATERIALS FOR SKIN TISSUE ENGINEERING: REPAIR AND REGENERATION - A SHORT REVIEW”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 11, Nov. 2018, pp. 16-20, doi:10.22159/ajpcr.2018.v11i11.26343.
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