ANTIDIABETES ACTIVITIES EXTRACT HEXANE FROM THE PEELS OF ARTOCARPUS CAMANSI BLANCO FRUIT

Rosnani Nasution; Chairani Nur Fitrah; Hira Helwati; Murniana Murniana; Bastian Arifin; Cutchamzurni Cutchamzurni; Yose Rizal; Marianne Marianne

doi:10.22159/ajpcr.2018.v11s1.26554

Authors

Rosnani Nasution Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Chairani Nur Fitrah Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Hira Helwati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Murniana Murniana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Bastian Arifin Department of Chemical Engineering, Faculty of Engineering, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Cutchamzurni Cutchamzurni Department of Plant Protection, Faculty of Agriculture, Syiah Kuala University, Banda Aceh, 23111, Indonesia.
Yose Rizal Department of Computer System, Faculty of Computer Science, Pembangunan Panca Budi University, Medan, Indonesia.
Marianne Marianne Department of Pharmacology, Faculty of Pharmacy,University of Sumatera Utara, Medan, Indonesia,

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26554

Keywords:

n-hexane extract, Artocarpus camansi, Antidiabetic, Glucose tolerance

Abstract

Â Objective: The purpose of this research is to know the chemical compound from the n-hexane extract of Kulu (Artocarpus camansi Blanco) fruit peels and to know its antidiabetes activity against male Swiss Webster mice.

Methods: Samples were macerated with n-hexane solvent, then the extracts were obtained, was characterization with gas chromatography-mass spectrometry (GC-MS), and then the extract separated by column chromatography. The results obtained were tested by antidiabetes action with glucose tolerance method.

Results: Anti-diabetic activity test result showed that the most active sample to decrease the blood glucose of mice was n-hexane crude extract, at minute 90th, activeness was 65.59%, compared to glibenclamide, then group of fraction A, at minute 90th, activeness 65.58% compared glibenclamide, whereas isolate D11, its activity is 60.11% and 60.12%, at minute 60th and 90 min compared to glibenclamide. Concentrated extracts were characterized by GC-MS, shows the major compounds are hexadecanoic acid methyl ester (30.14%), 9,12-octadecadienoyl chloride, (Z, Z) - (8.44%) and 9-octadecenoic acid, methyl ester (30,91%) also were obtained a compound like an Î²-amyrin acetate.

Conclusion: The most active sample to decrease the blood glucose of mice was n-hexane crude extract, and the major compounds are hexadecanoic acid methyl ester (30.14%), 9,12-octadecadienoyl chloride, (Z, Z) - (8.44%) and 9-octadecenoic acid, methyl ester (30.91%).

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