• Nasiruddin Ahmad Farooqui
  • Akalanka Dey
  • Singh Gn
  • Easwari Ts



 Objective: The aim was to develop and validate a new, rapid, and highly sensitive high performance liquid chromatography (HPLC) method for the quantitative estimation of vasicine in bulk and pharmaceutical dosage form, according to International Conference on Harmonization (ICH) guideline.

Methods: The chromatographic separation was achieved on an agilent 1200 series HPLC system phenyl (250 mm×4.6 mm×5 μm) column packing, using a mobile phase consisting of hexane sulphonic acid-acetonitrile-acetic acid (60:20:1; v/v/v) in isocratic mode. The flow rate was set at 1.0 ml/minute, and ultraviolet detection was monitored at 300 nm.

Results: The method was linear in the concentration range of 3.125-200 ppm/ml with a correlation coefficient of 0.999. The retention time for vasicine was found to be 5.30±0.05 minutes. The main recoveries obtained in the range of 90.476-107.1%, shows that the developed method was accurate and precise (<2% relative standard deviation). The lower limit of detection and limit of quantification were 3.0208 and 9.1541 μg/ml, respectively.

Conclusion: The proposed method met the general requirements with an acceptable performance for validation. This selective method is found to be reliable, accurate, and effectively used for the vasicine. The result showed that the method is achieved a good performance with simple, rapid and accurate characteristics for quantification of vasicine in pharmaceutical preparations. The proposed method can be employed for the routine analysis of the quality of herbal extracts and in formulations.

Keywords: High-performance liquid chromatography, Vasicine, International conference on harmonisation guidelines, Validation, Phenyl column.



Ahmad S. Introduction to Pharmacognosy. New Delhi: IK International Publisher; 2012.

Anjaria J, Parabia M, Bhatt G, Khamar R. Nature Heals. A Glossary of Selected Indigenous Medicinal Plants of India. Ahmedabad: SRISTI Innovations; 2002.

Kapoor LD. Hand Book of Ayurvedic Medicinal Plants. Boca Raton, FL: CRC Press; 2001.

Shinawie A. Wonder drugs of medicinal plants. Ethnobotany. Mol Cell Biochem 2002;213:99-109.

Rawat MS, Pant G, Badoni S, Negi YS. Biochemical investigations of some wild fruits of Garhwal Himalayas. Prog Hortic 1994;26:35-40.

Gupta OP, Sharma ML, Ghatak BJ, Atal CK. Pharmacological investigations of vasicine and vasicinone – The alkaloids of Adhatoda vasica. Indian J Med Res 1977;66(4):680-91.

Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z. Medicinal plants in therapy. Bull World Health Organ 1985;63(6):965-81.

Anonymous. Quality Control Methods for Medicinal Plant Material. WHO/PHARM/92.559. Geneva: WHO/PHARM; 1992.

Srivastava S, Verma RK, Gupta MM, Singh SC, Kumar S. HPLC determination of vasicine and vasicinone in Adhatoda vasica with photo diode array detection. J Liq Chromatogr Relat Technol 2001;24:153-9.

Narayana DB, Agarwal S, Luthra SK, Srinivas NS. A HPLC method for the quantitative analysis of vasicine in Adhatoda vasica. Indian Drugs 1995;32:583-6.

Brain KR, Thapa BB. High performance liquid chomatographic determination of vasicine and vasicinone in Adhatoda vasica Nees. J Chromatogr A 1983;258:183-8.

ICH, Harmonized Tripartite Guideline. Validation of Analytical Procedure: Methodology (Q2B). International Conference on Harmonization; 1997.



How to Cite

Farooqui, N. A., A. Dey, S. Gn, and E. Ts. “DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR QUANTITATIVE ESTIMATION OF VASICINE IN BULK AND PHARMACEUTICAL DOSAGE FORM”. Asian Journal of Pharmaceutical and Clinical Research, vol. 7, no. 5, Nov. 2014, pp. 235-40,



Original Article(s)