• Suriyakala Perumal Chandran Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
  • Kannikaparameswari Nachimuthu Department of Biochemistry, Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.



Papain, Solid lipid nanoparticles, Cytotoxic potential, Proteolytic enzyme, In vitro drug release


Objective: Colorectal cancer is one of the most commonly diagnosed cancer and also most common gastrointestinal malignancy with high prevalence rate in the younger population. Usually, cancer cells are surrounded by a fibrin coat which is resistant to fibrinolytic degradation. This fibrin coat is act as self-protective against natural killing mechanism. The main objective was to prepare papain-loaded solid lipid nanoparticles (P-SLN) by melt dispersion-ultrasonication method and investigated the cytotoxic efficacy against colorectal adenocarcinoma (human colorectal adenocarcinoma [HCT 15]) cells.

Methods: Optimized polymer ratio was characterized by differential scanning calorimetry, Fourier-transform infrared, X-ray diffraction, scanning electron microscopy, entrapment efficiency, particle size and zeta potential analysis, in vitro drug release, and in vitro cytotoxicity studies on HCT-15 colorectal adenocarcinoma cells.

Results: The results showed that the particle size, morphological character and zeta potential value of optimized batch P-SLN were 265 nm, spherical and −26.5 Mv, respectively. The in vitro drug profile of P-SLN exhibited that it produced sustain drug release, and the cell viability of HCT-15 against P-SLN shown better efficacy than pure papain enzyme.

Conclusion: P-SLNs were successfully prepared and investigated the in vitro drug release and in vitro cell viability against HCT-15 cell line.


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How to Cite

Chandran, S. P., and K. Nachimuthu. “FORMULATION AND CHARACTERIZATION OF PAPAIN LOADED SOLID LIPID NANOPARTICLES AGAINST HUMAN COLORECTAL ADENOCARCINOMA CELL LINE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 10, Oct. 2018, pp. 393-9, doi:10.22159/ajpcr.2018.v11i10.27258.



Original Article(s)