IN VITRO ANTIBACTERIAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC EXTRACT OF LEAVES OF ELETTARIA CARDAMOMUM L. MATON
Objective: The objective of the present study is to determine the possible phytoconstituents identified by gas chromatography and mass spectrometry (GC-MS) analysis of an ethanolic leaves extract of Elettaria cardamomum L. Maton.
Methods: The extraction of E. cardamomum was done by cold solvent extraction system at room temperature. GC-MS analysis of lyophilized ethanolic leaves extract of plant samples was carried out by GC-MS-GC Clarus 500 Perkin Elmer.
Results: In E. cardamomum, 21 phytochemicals were identified among which retinal, 9-cis-showed the highest area (44.86%) and benzeneethanamine, Î±-methyl- showed the lowest area (0.12%). The major compounds identified were retinal, 9-cis-, 1-heptatriacotanol, phytol, n-hexadecanoic acid, naphthalene,decahydro-1,1,4atrimethyl-6-methylene-5-(3-methyl-2-4-pentadienyl)-[4aS-(4aÎ±,5Î±,8aÎ±)]-, Î²-pinene, 2H-pytan-3-ol,6- ethenyltetrahydro-2,2,6-trimethyl-and cyclopropane, trimethanol, (2-methyl-1-propanylidene).
Conclusion: GC-MS analysis revealed the presence of hydrocarbon alkane, ester, terpenes, phenolic compounds, steroids, and fatty acids in E. cardamomum. These active phytoconstituents contribute to the medicinal efficacy of the plant, and the plant can be used for the sourcing of these compounds.
2. Ayyanar M, Ignacimuthu S. Ethnobotanical survey of medicinal plants commonly used by Kani tribals in Tirunelveli hills of Western Ghats, India. J Ethnopharmacol 2011;134:851-64.
3. Pandey N, Meena RP, Rai SM, Pandey-Rai S. Medicinal plants derived nutraceuticals: A re-emerging health aid. Int J Pharma Bio Sci 2011;2:420-41.
4. Dimple, Kumar A, Kumar V, Tomer V. Traditional medicinal systems for treatment of diabetes mellitus: A review. Int J Pharm Pharm Sci 2018;10:7-17.
5. Nagja T, Vimal K, Sanjeev A. Myristica fragrans: A Comprehensive review. Int J Pharm Pharm Sci 2016;8:27-30.
6. Bikash G, Biswajit D, Sujata A, Aditya B. Preliminary phytochemical screening and in vitro anti-microbial activity of ethanolic extract of Delonix regia (Hook) Raf. Bark (Family-Leguminosae). Int J Curr Pharm Res 2018;10:43-6.
7. Pawar AT, Vyawahare NS. Phytochemical and pharmacological profile of Biophytum sensitivum (L) DC. Int J Pharm Pharm Sci 2014;6:18-22.
8. Tamizhazhagan V, Pugazhendy K. Ethnobotanical and phytopharmacological review of Pisonia alba span. Asian J Pharm Clin Res 2017;10:69-71.
9. Dilworth LL, Riley CK, Stennett DK. Plant constituents: Carbohydrates, oils, resins, balsams, and plant hormones. In: Badal S, Delgoda R, editors. Pharmacognosy: Fundamentals, Applications and Strategies. Ch. 5. Tokyo: Academic Press; 2017. p. 61-80.
10. Edayadulla N, Ramesh P. Antibacterial activity of various stem extracts of Dalbergia coromandeliana. Asian Pac J Trop Biomed 2012;2:S1388-91.
11. Gomathi D, Kalaiselvi M, Ravikumar G, Devaki K, Uma C. GC-MS analysis of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides (L.) L. J Food Sci Technol 2015;52:1212-7.
12. Uma B, Prabhakar K, Rajendran S, Sarayu LY. Studies on GC/MS spectroscopic analysis of some bioactive antimicrobial compounds from Cinnamomum zeylanicum. J Med Plants 2009;8:125-31.
13. Farooq A, Ali A, Khalid MA, Anwar-ul-Hassan G. Cardamom (Elettaria cardamomum Maton) oils. In: Victor RP, editor. Essential Oils in Food Preservation, Flavor and Safety. Amsterdam: Academic press; 2016. p. 295-301.
14. Shetty P, Rao SN, Rani NM. Preliminary phytochemical screening of ethanolic extract of Elettaria cardamomum fruits. Int J Univ Pharm Bio Sci 2013;2:584-90.
15. Khan A, Khan QJ, Gilani AH. Pharmacological basis for the medicinal use of cardamom in asthma. Bangladesh J Pharm 2011;6:34-7.
16. Shailesh. Preliminary phytochemical and antimicrobial screening of Syzygium aromaticum, Elettaria cardamomum and Piper nigrum extracts. J Pharm Phytochem 2015;4:85-9.16.
17. Slack CB, Peutherer TT. Medical and Microbiology, a Guide to Microbial Infections, Pathogenesis, Immunity, Laboratory Diagnosis and Control. London: Churchill Livingston; 2002. p. 225-85.
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