COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS

  • Bosco Lawarence Department of Botany and Biotechnology, Government Arts College, Trivandrum - 695 014, Kerala, India. http://orcid.org/0000-0002-2519-2147
  • Murugan K Department of Botany and Biotechnology, Government Arts College, Trivandrum - 695 014, Kerala, India.

Abstract

Objective: The objective of the present work is to isolate, purify, and fractionate anthocyanin from selected Osbeckia species and also to compare the analgesic and anti-inflammatory potentiality using animal models.

Methods: Methodologies include extraction of anthocyanin from the in vitro callus culture of Osbeckia aspera and Osbeckia reticulata, purification using amberlite column chromatography, and fractionation by liquid chromatography-tandem mass spectrometry. The analgesic activity was determined by tail immersion method, analgesy meter, hot plate, and acetic acid-induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced paw inflammation in mice.

Results: Anthocyanin-producing callus cultures were established in MS medium fortified with various combinations of phytohormones and sucrose. Optimal callus formation in O. aspera was initiated on cultures containing 0.5 mg/L of 2, 4-D, and 0.5 mg/L 6-benzylaminopurine (BA). In O. reticulata callus was initiated in the presence of 1.2 mg/L BA and 1.4 mg/L naphthalene acetic acid. The same hormonal combination on extended treatments turned the white friable callus into red compact callus. Anthocyanins obtained from Osbeckia species were purified and fractionated containing malvidin-3-diglucoside, delphinidin, cyanidin aglycone, and peonidin. Purified anthocyanin of O. aspera at the concentrations 50, 100, 150, and 200 mg/kg after 120 min exhibited significant analgesic activity by tail immersion method, in comparison to O. reticulata. However, with hot plate method, anthocyanin of O. reticulata produced a significant analgesic activity even at lower doses (50 and 100 mg/kg) after 120 min. However, in writhing test, theepurified anthocyanin of O. aspera significantly stopped the number of writhes at a dose of 200 mg/kg and also by the anthocyanin of O. reticulata. In the evaluation of anti-inflammatory effect using plethysmometer, anthocyanin at doses of 100,150 and 200 mg/kg started producing anti-inflammatory effect after 30 min, which lasted until 120 min.

Conclusion: It is concluded from the present study that the purified anthocyanin of Osbeckia possesses potent analgesic and anti-inflammatory activities.

Keywords: Osbeckia, Purified anthocyanin, In vitro culture, Analgesic activity, Anti-inflammatory activity, Acetic acid, Carrageenan.

Author Biographies

Bosco Lawarence, Department of Botany and Biotechnology, Government Arts College, Trivandrum - 695 014, Kerala, India.
Assistant Professor
Murugan K, Department of Botany and Biotechnology, Government Arts College, Trivandrum - 695 014, Kerala, India.
Principal

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Lawarence, B., and M. K. “COMPARISON OF ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF PURIFIED ANTHOCYANIN FROM OSBECKIA ASPERA (L.) BLUME AND OSBECKIA RETICULATA BEDD. USING ANIMAL MODELS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 106-11, doi:10.22159/ajpcr.2019.v12i1.27402.
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