PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF PORTULACA QUADRIFIDA LINN.
Objective: The objective of the study was to investigate in vitro antimicrobial activity against enterotoxigenic Escherichia coli and Bacillus subtilis and preliminary phytochemical screening of the leaves of Portulaca quadrifida (Linn.).
Methods: The solvent extract such as petroleum ether, methanol, and water on the leaves of P. quadrifida (Linn) was prepared by Soxhlet extraction (continuous hot percolation method). These solvent extracts were screened for antimicrobial activity against enterotoxigenic E. coli and B. subtilis at various concentrations and were measured by observing zone of inhibition in mm by disc diffusion method (cup plate method).
Results: The preliminary phytochemical screening revealed the flavonoids, fats, and oils in all extracts. Similarly, the presence of alkaloids and tannins was obtained in the petroleum ether and methanolic extracts, while the presence of glycosides was obtained in the methanolic and water extracts. Further, proteins and sterols were found in petroleum extracts. The results of antimicrobial activity shown that methanolic extracts of the plant leaf showed good antimicrobial activity and petroleum ether and water extract showed similar activity but less antimicrobial activity than methanolic extract. The antimicrobial activities of extracts were compared with standard antibiotic such as chloramphenicol.
Conclusion: P. quadrifida (Linn.) has broad-spectrum antimicrobial activity and a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control. The phytochemical analysis of the crude extracts of this plant indicates the presence of major phytoconstituents which may have been responsible for the observed antimicrobial property.
2. Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Allahbad: Lalit Mohan Basu, Publishers; 1984. p. 72-7.
3. Patil AG, Joshi VS, Koli SP, Patil D. Pharmacognistical and phytochemical analysis of Portulaca quadrifida Linn. Res J Pharm Biol Chem Sci 2012;3:90-100.
4. Syed KM, Ahmed LT, Paramjyothy S. J Appl Pharm Res Pharm Technol 2010;2:1386-90.
5. Hoffman BR, Alas D, Blanco K, Wilder Hold N, Lewis RE, Williams L. Pharm Biol 2004;42:3-17. 6. Khandelwal KR. Practical Pharmacognosy: Techniques and Experiments. 21st ed. Pune: Nirali Prakashan Publishers; 2011. p. 10.1-10.4.
7. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 46th ed. India: Nirali Prakashan Publishers; 2007.
8. Kokare CR. Pharmaceutical Microbiology Experiments & Techniques. 2nd ed. Nashik, India: Career Publishers; 2007. p. 55-7.
9. Kokare CR. Pharmaceutical Microbiology & Biotechnology. 3rd ed. Pune: Nirali Prakashan Publishers; 2006. p. 21.1-21.6.
10. Bashyal S, Guha A. Evaluation of Trachyspermum ammi seeds for antimicrobial activity and phytochemical analysis. Asian J Pharm Clin Res 2018;11:274-7.
11. Eltayeb IM, Abdeen Salim Hamid A. Phytochemical screening and antimicrobial activity of Thuja occidentalis seeds extracts against the isolated common skin infecting microorganisms. Int J Pharm Pharm Sci 2017;9:131-4.
12. Vadnere Gautam P, Rageeb Usman Md, Lodhi S, Vaishali P. Phytochemical investigation and in vitro antimicrobial screening of Santalum album seeds extracts. Int J Pharm Pharm Sci 2017;9:117-24.
13. Das M, Ashok Kumar D. Phyto-pharmacological review of Portulaca quadrifida linn. J Appl Pharm Res 2013;1:1-4.
14. Goel RK, Sairam K, Babu MD, Tavares IA, Raman A. In vitro evaluation of Bacopa monniera on anti-Helicobacter pylori activity and accumulation of prostaglandins. Phytomedicine 2003;10:523-7.
15. Kamil M, Liyakha T, Md Ahmed LT, Paramjyothi S. Neuropharmacological activity. Int J Pharm Technol Res 2010;2:1386-90.
16. Dar A, Channa SJ. Gastrointestinal disorders. Ethnophamacol 1999;66:167-74.
17. Parmart NS, Prakash S. Screening Methods in Pharmacology. New Delhi: Narosa Publishing House; 2006. p. 90-1.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.